دورية أكاديمية
Cell death induced by 2-phenylethynesulfonamide uncovers a pro-survival function of BAX
| العنوان: | Cell death induced by 2-phenylethynesulfonamide uncovers a pro-survival function of BAX |
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| المؤلفون: | Mattiolo, Paolo, Barbero-Farran A., Amigó J., Ripamonti M., Ribas i Fortuny, Judit, Boix Torras, Jacint |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2015 |
| المجموعة: | Universitat de Lleida: Repositori Obert UdL |
| مصطلحات موضوعية: | PES (2-phenylethynesulfonamide), pifithrin-mu, mitochondrial dynamics, Bcl-2, Necrosis, Mitocondris, Mort cel·lular, Mitochondria, Cell death |
| الوصف: | PES (2-phenylethynesulfonamide) was initially identified as an inhibitor of p53 translocation to mitochondria and named Pifithrin-µ. Further studies showed that PES selectively killed tumour cells and was thus a promising anticancer agent. PES-induced cell death was characterised by a non-apoptotic, autophagosome-rich phenotype. We observed this phenotype via electron microscopy in wild type (wt) and double Bax-/- Bak-/- (DKO) mouse embryonic fibroblasts (MEFs) treated with PES. We excluded the involvement of effector caspases, BAX and BAK, in causing PES-triggered cell death. Therefore, apoptosis was ruled out as the lethal mode of action of PES. Surprisingly, MEFs containing BAX were significantly protected from PES treatments. BAX overexpression in Bax-/- MEFs confirmed this pro-survival effect. Moreover, this protective effect required the ability of BAX to localise to mitochondrial membranes. Conversely, mitochondrial fusion induced by treatment with Mdivi-1 conferred increased resistance to MEFs subjected to PES treatment. The involvement of BAX in the regulation of mitochondrial dynamics has been reported. We propose the promotion of mitochondrial fusion by BAX to be the pro-survival function attributed to BAX. ; This work was supported by the Spanish Government, MINECO (Ministerio de Economía y Competitividad), project SAF2011-29730. Paolo Mattiolo was supported by an FI fellowship from AGAUR (Generalitat de Catalunya). |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0304-3835 |
| العلاقة: | info:eu-repo/grantAgreement/MICINN//SAF2011-29730/ES/REGULACION DE LA AUTOFAGIA POR LOS GENES TMEM49 Y MIR-21/; Versió postprint del document publicat a: https://doi.org/10.1016/j.canlet.2014.07.037Test; Cancer Letters, 2014, vol. 354, num. 1, p. 115-121; http://hdl.handle.net/10459.1/47849Test; https://doi.org/10.1016/j.canlet.2014.07.037Test |
| DOI: | 10.1016/j.canlet.2014.07.037 |
| الإتاحة: | https://doi.org/10.1016/j.canlet.2014.07.037Test http://hdl.handle.net/10459.1/47849Test |
| حقوق: | (c) Elsevier Ireland, 2014 ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.8B37E75B |
| قاعدة البيانات: | BASE |
| تدمد: | 03043835 |
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| DOI: | 10.1016/j.canlet.2014.07.037 |