دورية أكاديمية
The preservation of phenotype and functionality of dendritic cells upon phagocytosis of polyelectrolyte-coated PLGA microparticles
| العنوان: | The preservation of phenotype and functionality of dendritic cells upon phagocytosis of polyelectrolyte-coated PLGA microparticles |
|---|---|
| المؤلفون: | Fischer, Stefan, Waeckerle-Men, Ying, Merkle, Hans P., Gander, Bruno, Uetz-von Allmen, Edith, Gröttrup, Marcus |
| المصدر: | Biomaterials. 2007, 28(6), pp. 994-1004. ISSN 0142-9612. Available under: doi:10.1016/j.biomaterials.2006.10.034 |
| سنة النشر: | 2007 |
| المجموعة: | University of Konstanz: Konstanz Online Publication Server (KOPS) |
| مصطلحات موضوعية: | Dendritic cells, Immunomodulation, Microsphere, ddc:570 |
| الوصف: | Biodegradable microparticles (MP) represent a promising and efficient delivery system for parenteral vaccination. Recently, MP have also been explored as tool for the ex vivo antigen loading of professional antigen presenting cells such as dendritic cells (DC) to be used as cellular vaccines. The purpose of this study was to investigate various polycationic coatings on poly(lactide-co-glycolide) (PLGA) MP, with regard to their effect on phenotypic and functional maturation of monocyte-derived DC (MoDC) that had previously been loaded with the MP in vitro. The preparation and concomitant coating of the PLGA was performed by means of a solvent extraction/evaporation method using a recently developed microextrusion-based technique. The polyelectrolytes tested for MP coating encompassed aminodextran, chitosan, poly(ethylene imine) (PEI), poly(L-lysine) and protamine. Uncoated and differently coated PLGA MP were fed to immature MoDC, which ingested efficiently the different MP types irrespective of their surface coating. The MPloaded immature MoDC were then matured with the help of a cytokine/PGE-2 maturation cocktail. Here, the presence of the ingested MP did not affect the MoDC maturation in terms of expression of the surface markers CD80, CD83, CD86, HLA-DR and MMR, irrespective of the MP surface coating. Importantly, none of the PLGA MP types alone induced significant maturation of MoDC in the absence of the maturation cocktail. MP-loaded and subsequently matured MoDC expressed high levels of the chemokine receptor CCR7, whose functional activity was evidenced by the migration of MoDC towards CCL21, irrespective of the presence of ingested MP. Further, MP-loaded and subsequently matured MoDC also secreted comparable amounts of IL-10 and IL-12p70, irrespective of the presence of ingested MP except for PEI-coated PLGA MP, which enhanced significantly the secretion of IL-12p70 in mature MoDC. In conclusion, phenotypic and functional maturation of MoDC by means of a maturation cocktail remained unchanged irrespective ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | http://nbn-resolving.de/urn:nbn:de:bsz:352-220774Test; http://dx.doi.org/10.1016/j.biomaterials.2006.10.034Test; 497075865 |
| DOI: | 10.1016/j.biomaterials.2006.10.034 |
| الإتاحة: | https://doi.org/10.1016/j.biomaterials.2006.10.034Test http://nbn-resolving.de/urn:nbn:de:bsz:352-220774Test |
| حقوق: | https://rightsstatements.org/page/InC/1.0Test/ |
| رقم الانضمام: | edsbas.9AD834F2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.biomaterials.2006.10.034 |
|---|