Structure and Biological Activity of a Turripeptide from Unedogemmula bisaya Venom
| العنوان: | Structure and Biological Activity of a Turripeptide from Unedogemmula bisaya Venom |
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| المؤلفون: | Gisela P. Concepcion, Joanna Gajewiak, Louie D. Carpio, Shrinivasan Raghuraman, Julita S. Imperial, Malem S. Flores, Estuardo López-Vera, Norelle L. Daly, Olena M. Filchakova, Baldomero M. Olivera, Sean Christensen, Carla A. Omaga, Samuel S. Espino |
| المصدر: | Biochemistry. 56:6051-6060 |
| بيانات النشر: | American Chemical Society (ACS), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Male, Models, Molecular, 0301 basic medicine, Peptide, Venom, Receptors, Nicotinic, Biology, Biochemistry, Mice, Xenopus laevis, 03 medical and health sciences, Dorsal root ganglion, Ganglia, Spinal, medicine, Animals, Receptor, Cells, Cultured, Neurons, chemistry.chemical_classification, Mice, Inbred ICR, 030102 biochemistry & molecular biology, Conus Snail, Biological activity, Peptide Fragments, 3. Good health, Nicotinic acetylcholine receptor, 030104 developmental biology, medicine.anatomical_structure, chemistry, Oocytes, Excitatory postsynaptic potential, Calcium, Female, Conotoxins, Cysteine |
| الوصف: | The turripeptide ubi3a was isolated from the venom of the marine gastropod Unedogemmula bisaya, family Turridae, by bioassay-guided purification; both native and synthetic ubi3a elicited prolonged tremors when injected intracranially into mice. The sequence of the peptide, DCCOCOAGAVRCRFACC-NH2 (O = 4-hydroxyproline) follows the framework III pattern for cysteines (CC–C–C–CC) in the M-superfamily of conopeptides. The three-dimensional structure determined by NMR spectroscopy indicated a disulfide connectivity that is not found in conopeptides with the cysteine framework III: C1–C4, C2–C6, C3–C5. The peptide inhibited the activity of the α9α10 nicotinic acetylcholine receptor with relatively low affinity (IC50, 10.2 μM). Initial Constellation Pharmacology data revealed an excitatory activity of ubi3a on a specific subset of mouse dorsal root ganglion neurons. |
| تدمد: | 1520-4995 0006-2960 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c68718e7e0bcbdd26e9676f271ffb156Test https://doi.org/10.1021/acs.biochem.7b00485Test |
| رقم الانضمام: | edsair.doi.dedup.....c68718e7e0bcbdd26e9676f271ffb156 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15204995 00062960 |
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