التفاصيل البيبلوغرافية
| العنوان: |
Gracillin inhibits apoptosis and inflammation induced by lipopolysaccharide (LPS) to alleviate cardiac injury in mice via improving miR-29a. |
| المؤلفون: |
Song, Yin-xue1 (AUTHOR), Ou, Yan-mian1,2 (AUTHOR), Zhou, Jing-yu1,3 (AUTHOR) zt.1215@qq.com |
| المصدر: |
Biochemical & Biophysical Research Communications. Mar2020, Vol. 523 Issue 3, p580-587. 8p. |
| مصطلحات موضوعية: |
*LIPOPOLYSACCHARIDES, *LACTATE dehydrogenase, *CELL death, *HEART injuries, *MICE, *WOUNDS & injuries |
| مصطلحات جغرافية: |
AGRA (India) |
| مستخلص: |
Sepsis induces critical myocardial dysfunction, resulting in an increased mortality. Gracillin (GRA) is a natural steroidal saponin, showing strong capacities of anti-inflammation, but its pharmacological effects on lipopolysaccharide (LPS)-induced acute cardiac injury still remain unclear. In this study, we attempted to explore if GRA was effective to attenuate cardiac injury in LPS-challenged mice and the underlying mechanisms. First, we found that GRA treatments markedly up-regulated the expression of miR-29a in cardiomyocytes. LPS-induced cytotoxicity in cardiomyocytes was significantly alleviated by GRA treatment, as evidenced by the improved cell viability and reduced lactate dehydrogenase (LDH) release. In addition, LPS-triggered apoptotic cell death was clearly ameliorated in cardiomyocytes co-treated with GRA. Notably, LPS-exposed cells showed significantly reduced expression of miR-29a, while being rescued by GRA treatment. In vivo , LPS apparently impaired cardiac function in mice, which was, however, alleviated by GRA administration. In addition, GRA markedly attenuated apoptosis in hearts of LPS-challenged mice by decreasing the expression of cleaved Caspase-3. LPS-triggered inflammatory response in cardiac tissues was also suppressed by GRA through blocking nuclear factor κB (NF-κB) signaling pathway. We also found that miR-29a expression was highly reduced in hearts of LPS-treated mice but was rescued by GRA pretreatment. Besides, miR-29a mimic alleviated LPS-induced apoptosis and inflammation in cardiomyocytes; however, LPS-caused effects were further accelerated by miR-29a. Of note, the protective effects of GRA on LPS-injured cardiac tissues were significantly abrogated by miR-29a suppression. In conclusion, our findings demonstrated that GRA exerted an effective role against LPS-induced acute cardiac injury through impeding apoptosis and inflammation regulated by miR-29a. • Gracillin reduces apoptosis in LPS-incubated cardiomyocytes. • up-regulation of miR-29a alleviates apoptosis and inflammatory response in LPS-stimulated cardiomyocytes. • Gracillin alleviates LPS-induced acute heart injury through improving miR-29a expression. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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