التفاصيل البيبلوغرافية
| العنوان: |
Increased mGlu5 mRNA expression in BLA glutamate neurons facilitates resilience to the long-term effects of a single predator scent stress exposure. |
| المؤلفون: |
Shallcross, John1 (AUTHOR), Wu, Lizhen1 (AUTHOR), Wilkinson, Courtney S.1,2 (AUTHOR), Knackstedt, Lori A.1,2 (AUTHOR), Schwendt, Marek1,2 (AUTHOR) schwendt@ufl.edu |
| المصدر: |
Brain Structure & Function. Sep2021, Vol. 226 Issue 7, p2279-2293. 15p. |
| مصطلحات موضوعية: |
*FLUORESCENCE in situ hybridization, *GLUTAMATE receptors, *SPRAGUE Dawley rats, *MESSENGER RNA, *GLUTAMIC acid, *POST-traumatic stress disorder, *AMYGDALOID body |
| مستخلص: |
Post-traumatic stress disorder (PTSD) develops in a subset of individuals exposed to a trauma with core features being increased anxiety and impaired fear extinction. To model the heterogeneity of PTSD behavioral responses, we exposed male Sprague–Dawley rats to predator scent stress once for 10 min and then assessed anxiety-like behavior 7 days later using the elevated plus maze and acoustic startle response. Rats displaying anxiety-like behavior in both tasks were classified as stress Susceptible, and rats exhibiting behavior no different from un-exposed Controls were classified as stress Resilient. In Resilient rats, we previously found increased mRNA expression of mGlu5 in the amygdala and prefrontal cortex (PFC) and CB1 in the amygdala. Here, we performed fluorescent in situ hybridization (FISH) to determine the subregion and cell-type-specific expression of these genes in Resilient rats 3 weeks after TMT exposure. Resilient rats displayed increased mGlu5 mRNA expression in the basolateral amygdala (BLA) and the infralimbic and prelimbic regions of the PFC and increased BLA CB1 mRNA. These increases were limited to glutamatergic cells. To test the necessity of mGlu5 for attenuating TMT-conditioned contextual fear 3 weeks after TMT conditioning, intra-BLA infusions of the mGlu5 negative allosteric modulator MTEP were administered prior to context re-exposure. In TMT-exposed Resilient rats, but not Controls, MTEP increased freezing on the day of administration, which extinguished over two additional un-drugged sessions. These results suggest that increased mGlu5 expression in BLA glutamate neurons contributes to the behavioral flexibility observed in stress-Resilient animals by facilitating a capacity for extinguishing contextual fear associations. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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