نتائج البحث - "Lactate dehydrogenase"

1

Development of a Rapid Intraoperative Point-of-Care Method Using Tissue Suspension to Differentiate Parathyroid Tissue: A Possible Substitute for Frozen Sections

المؤلفون: Dai Takeuchi, Toyone Kikumori, Masahiro Shibata

المصدر: World Journal of Surgery. 45(7):2142-2145

الوصف: Background: We reported that aspartate aminotransferase (AST)/lactate dehydrogenase (LDH) ratio of a tissue suspension can precisely differentiate normal and hyperfunctioning parathyroid tissue (PT) from other tissues. However, in these studies, LDH and AST were measured using the standard method for blood samples, with a turnaround time of approximately 1 h, hampering clinical application. Here, we developed a rapid and robust method to differentiate PT instead of using frozen sections. Methods: Excised specimens from 28 patients (n = 69) who underwent thyroid or parathyroid surgery between October 2019 and April 2020 were analyzed. AST and LDH were measured in suspensions of PT or other tissues, using both the standard method in the in-facility laboratory and a point-of-care testing device (NX500, Fujifilm, Japan). Results and conclusions: A good correlation was found between the standard method and NX500 for AST and LDH levels >10 IU/L. In the analyses using 52 specimens with ≥ 10 IU/L of both AST and LDH measured using the NX500, PT was distinguished with 100% sensitivity and specificity using an optimal cutoff AST/LDH ratio of 0.48. The turnaround time was estimated to be less than 10 min. This method could be a cost- and labor-effective alternative to frozen sections to reduce the incidence of postoperative hypoparathyroidism and improve the outcome of primary hyperparathyroidism in low-resource areas.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::71cf4beb08b106b4fa98d9c3b19fd452Test
https://nagoya.repo.nii.ac.jp/records/2001616Test

2

Robust, quick, and convenient intraoperative method to differentiate parathyroid tissue

المؤلفون: Dai Takeuchi, Takahiro Inaishi, Noriyuki Miyajima, Masahiro Shibata, Toyone Kikumori

المصدر: Surgery. 167(2):385-389

الوصف: Background: Identification of parathyroid tissue during surgery is necessary for its preservation in situ or for autotransplantation to avoid postoperative hypoparathyroidism. Frozen sections are the gold standard for distinguishing parathyroid tissue from other tissues during thyroidectomy. Although frozen sections are very accurate, they are costly and require pathologists and technical staff. Parathyroid tissue is rich in mitochondria, which harbor Krebs-cycle enzymes such as aspartate aminotransferase. In contrast, lactate dehydrogenase is expressed ubiquitously. These 2 enzymes are measured routinely as “leaked” enzymes. We hypothesized that the aspartate aminotransferase–to–lactate dehydrogenase ratio in suspended tissue could distinguish parathyroid tissue from other tissues. Methods: We analyzed 94 specimens (43 parathyroid, 19 thyroid cancers, 13 normal lymph nodes, 10 adipose, 6 thyroid, and 3 miscellaneous tissues) from 55 patients who underwent thyroid or parathyroid surgery between March 2018 and June 2019 in our institution. Trace amounts of remnant parathyroid tissue from autotransplantation specimens were suspended in 1 mL of normal saline and measured for aspartate aminotransferase and lactate dehydrogenase. Approximately 1 mm3 of apparently distinct tissue minced by scissors (eg, thyroid gland, metastatic lymph node, etc) or washouts of needles used for preoperative aspiration biopsy were also measured for comparison. Results: The aspartate aminotransferase–to–lactate dehydrogenase ratios in suspended parathyroid tissue specimens were consistently greater than those of other tissues (P < .001, Mann-Whitney test); 0.27 was the optimal cutoff value with 100% sensitivity and specificity. Conclusion: This method distinguished parathyroid tissue quickly and conveniently from other tissues intraoperatively with minimum cost and without dedicated pathologic staff. This methodology may serve useful in decreasing the incidence of postoperative hypoparathyroidism, especially in settings with limited access to pathologists.
ファイル公開：2021-02-01

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f43a7cd908ed564dffafe237659841f1Test
https://nagoya.repo.nii.ac.jp/records/30104Test

3

Impact of Cold Ischemia Time on the Function of Liver Grafts Preserved With Custodiol

المؤلفون: Leonardo T. Mota, Regina Gomes dos Santos, Tércio Genzini, Juan Rafel Branez, Marcelo Perosa de Miranda, Beimar Edmundo Zeballos Sempertegui, Tiago Careli de Almeida, Fernanda Danziere, Francisco Antonio Sergi Filho, Marina Guitton Rodrigues, Paula Marcela Vilela Castro

المصدر: Transplantation Proceedings. 53:661-664

الوصف: This study aimed to evaluate how cold ischemia time (CIT) interferes with liver graft function in the first 7 days after surgery for Custodiol (HTK) preserved organs.This retrospective observational study analyzed the medical records of 38 transplantation patients at Hospital Leforte Liberdade, São Paulo, in 2018. The study population was divided into 2 groups (group A, CIT 8 hours; group B, CIT8 hours). Postoperative parameters-such as international normalized ratio, total bilirubin, aspartate aminotransferase/alanine aminotransferase, alkaline phosphatase, gamma glutamyl transferase (GGT), lactate dehydrogenase, lactate, creatinine, red blood cell transfusion, need for hemodialysis, use of vasoactive drugs, endotracheal intubation time, length of stay in the intensive care unit (ICU), and length of hospital stay-were compared.Group A (CIT 8 hours) presented less need for red blood cell transfusions (odds ratio 0.29; confidence interval 0.06-0.98; P = .04), had a shorter hospital stay (P = .024), and had lower levels of total bilirubin (P = .05) and GGT (P = .05) in the first 7 postoperative days. The other variables showed no statistically significant difference.In livers preserved with Custodiol, CIT8 hours generated higher levels of total bilirubin and GGT in the postoperative period, in addition to higher hospital costs; greater need for red blood cell transfusions; and longer hospitalization, including longer stays in the ICU.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c39ae572d79569248d53f518761264eTest
https://doi.org/10.1016/j.transproceed.2020.03.002Test

4

Comparison between Different Tricuspid Valve Procedures through Postoperative Inflammation and Myocardial Enzymes

المؤلفون: Zhigang Zhang, Shixiong Wei, Zuoyong Sun

المصدر: Brazilian Journal of Cardiovascular Surgery
Brazilian Journal of Cardiovascular Surgery, Volume: 36, Issue: 2, Pages: 212-218, Published: 18 SEP 2020
Brazilian Journal of Cardiovascular Surgery v.36 n.2 2021
Sociedade Brasileira de Cirurgia Cardiovascular (SBCCV)
instacron:SBCCV
Brazilian Journal of Cardiovascular Surgery, Vol 36, Iss 2, Pp 212-218 (2020)
Brazilian Journal of Cardiovascular Surgery, Issue: ahead, Published: 18 SEP 2020

مصطلحات موضوعية: Male, medicine.medical_specialty, RD1-811, medicine.medical_treatment, Heart Valve Diseases, 030204 cardiovascular system & hematology, 03 medical and health sciences, chemistry.chemical_compound, Leukocyte Count, 0302 clinical medicine, Troponin T, Lactate dehydrogenase, medicine, Thoracoscopy, Diseases of the circulatory (Cardiovascular) system, Humans, Thoracotomy, Creatine Kinase, Retrospective Studies, Heart Valve Prosthesis Implantation, Inflammation, Tricuspid valve, biology, medicine.diagnostic_test, business.industry, Retrospective cohort study, General Medicine, Sternotomy, Surgery, medicine.anatomical_structure, Treatment Outcome, chemistry, RC666-701, biology.protein, Creatine kinase, Original Article, Tricuspid Valve, Postoperative inflammation, Cardiology and Cardiovascular Medicine, business

الوصف: Introduction: The thoracoscopic procedure for tricuspid valve (TV) diseases is a minimally invasive method of treatment. This study focuses on comparing the changes in postoperative inflammatory reaction and myocardial injury markers after thoracoscopic and sternotomy/thoracotomy TV procedures. Methods: We retrospectively analyzed 88 patients (53 males, aged 50.9±16.2 years) with TV diseases (single-valve disease) (72 cases of TV plasty) between January 2018 and April 2019. A total of 56 patients underwent thoracoscopic procedure (50 cases of TV plasty). The leukocyte and C-reactive protein (CRP) levels were monitored as indicators of systemic inflammatory reaction. The lactate dehydrogenase, creatine kinase, creatine kinase myocardial band, aspartate aminotransferase, and troponin-T levels were recorded as markers of myocardial injury. Results: The CRP and white blood cells levels of patients in the sternotomy approach group were continuously higher than those in patients in the thoracoscopic approach group. And the levels of myocardial enzymes in patients in the thoracoscopic approach group were significantly lower than those in patients in the sternotomy approach group. Conclusion: Compared with sternotomy/thoracotomy procedures on TV, the thoracoscopic procedure can reduce postoperative myocardial injury significantly and systemic inflammatory reaction to a certain extent. It is technically feasible, safe, effective, and worthy of widespread adoption in clinical practice.

وصف الملف: text/html

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a98b2255f5a3df9c633051edc2c10b60Test
http://europepmc.org/articles/PMC8163275Test

5

Investigation into the Effect of Photodynamic Therapy and Cisplatin on the Cervical Cancer Cell Line (A2780)

المؤلفون: Zahra Bagheri-Hosseinabadi, Shahrzad Zadehmodarres, Mohammad Asnaashari, Nabaa Najjar, Fatemeh Javani Jouni, Jaber Zafari

المصدر: J Lasers Med Sci

الوصف: Introduction: Cervical cancer is recognized as one of the major causes of mortality among elderly women. Although there are several different therapeutic worldwide guidelines, many researchers have focused on screening new methodologies and technologies to elevate the efficiency of cervical cancer treatment. The simultaneous use of photodynamic therapy (PDT) along with chemotherapy as cisplatin has achieved good aims in the treatment of cervical cancer. Methods: A2780 cells were treated with cisplatin, photodynamic progress (laser with methylene blue as a photosensitizer compound) and a combination of cisplatin and PDT. The lithic effect of the laser, methylene blue and their combination and the IC50 value of cisplatin were calculated for each group. The amount of malondialdehyde (MDA) as membrane lipid peroxidation product and released lactate dehydrogenase was measured in the medium. The toxicity of each agent was evaluated by the MTT technique. Results: The results show that a combination of PDT and chemotherapeutic agent cisplatin caused a twofold decrease in viable cervical cancer cells compared to each therapeutic progress. The combination of both laser therapy and cisplatin enhanced cancer cell membrane disruption by increased membrane lipid peroxidation and apoptotic enzyme activation by the elevation of lactate dehydrogenase activity. Conclusion: The results indicated that cisplatin combined with PDT had a greater therapeutic effect on A2780 as a cervical cancer cell line. Therefore, PDT in combination with chemotherapy enhances the effectiveness of chemotherapeutic agents by the disruption of the cancer cell membrane and switching the apoptosis progress with less adverse effects.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67fe2f29f7ffd22c0e7d2abdfd029293Test
https://doi.org/10.34172/jlms.2020.s14Test

6

SARS-COV-2 infection in the perioperative of pulmonary lobectomy. About a case

المؤلفون: Fernando Valdivia, Irene Huaroto, Mardonio Euscatigue, Ricardo Taipe, Brenner Belloso

المصدر: International Journal of Surgery Case Reports

مصطلحات موضوعية: medicine.drug_class, medicine.medical_treatment, Antibiotics, Lung abscess, Case Report, SARS-COV-2, WHO, World Health Organization, Serology, Sepsis, AARB, Acid alcohol resistant bacillus, 03 medical and health sciences, 0302 clinical medicine, FiO2, Inspired fraction oxygen, Oxygen therapy, medicine, LDH, Lactate dehydrogenase, RT PCR, Real time polymerase chain reaction, C-RP, C-reactive protein, business.industry, COVID-19, Perioperative, SARS-COV-2, Severe acute respiratory syndrome coronavirus 2, COVID-19, Coronavirus disease-19, medicine.disease, SatO2, Oxyhemoglobin saturation, IgM/IgG, Immunoglobulins M and G, PaO2, Oxygen blood pressure, Pneumonia, 030220 oncology & carcinogenesis, Anesthesia, Lobectomy, Corticosteroid, 030211 gastroenterology & hepatology, Surgery, business

الوصف: Highlights • COVID-19 serological tests are not recommended for early diagnosis because they cause a lot of confusion. • Patients who require lung or chest surgery must undergo strict preoperative control and early detection of SARS-COV-2 infection. • The treatment of COVID-19 in the postoperative of patients undergoing pulmonary surgery should be based on respiratory rehabilitation and oxygen therapy. • The use of broad-spectrum antibiotics contributes to the treatment of secondary bacterial pneumonia. • In the preoperative period of patients requiring pulmonary surgery, the chest tomography should be considered with an antiquity of no more than 5 days.
Objective To describe the form of severe clinical presentation of SARS-COV-2 infection in the early phase, also the timely treatment of COVID-19 pneumonia in postoperative pulmonary lobectomy. Method Case report where the data were extracted from the clinical history and is in accordance with the SCARE 2018 criteria. Description of the case A 36-year-old man, with no significant history, who presented fever and mucopurulent expectoration of 1 month of evolution, received antibiotics for 4 weeks without response to treatment. Chest tomography shows cystic image with heterogeneous content in the left lower lobe. We decided to opt for surgery, previously 1 PCR in RT and 7 serological tests for COVID-19 were performed, the result of which was non-reactive. In the postoperative period, the patient developed fever and dyspnea on mild exertion, so a new serological test for COVID-19 was performed: IgM/IgG reactive, in addition chest tomography showed both lungs with “cracy paving” pattern. Discussion The serological tests did not contribute to a timely diagnosis of COVID-19 and generated confusion. We used oxygen therapy, broad spectrum antibiotics since the diagnosis of COVID-19. Likewise, respiratory physiotherapy was intensified even after discharge. Conclusion The early diagnosis and use of antibiotics at doses of sepsis, associated with corticosteroid pulses and respiratory physiotherapy improve COVID-19 pneumonia in postoperative lung surgery.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc62432c372c5c3f013e5e230341ca17Test
http://europepmc.org/articles/PMC7678424Test

7

Pembrolizumab plus platinum-based chemotherapy for unfavorable cancer of unknown primary site: Case report

المؤلفون: Norihiko Suzuki, Hideyuki Takeuchi, Junji Takahashi, Keigo Nakashima, Shinya Okada, Yutaka Suzuki, Hironori Ohdaira, Hiroshi Ishiguro, Teppei Kamada, Yuichi Nakaseko

المصدر: Annals of Medicine and Surgery

مصطلحات موضوعية: Oncology, medicine.medical_specialty, PD-L1, programmed death ligand 1, medicine.medical_treatment, NCCN, National Comprehensive Cancer Network, Case Report, Pembrolizumab, Pemetrexed, PET, positron emission tomography, Cancer of unknown primary, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Maintenance therapy, Internal medicine, medicine, Lung cancer, Platinum, Chemotherapy, LDH, lactate dehydrogenase, business.industry, ALK, anaplastic lymphoma kinase, CUP, cancer of unknown primary site, General Medicine, medicine.disease, TTF-1, thyroid transcription factor-1, Carboplatin, EGFR, epidermal growth factor receptor, CT, computed tomography, chemistry, 030220 oncology & carcinogenesis, CR, complete response, Adenocarcinoma, 030211 gastroenterology & hepatology, Surgery, CEA, carcinoembryonic antigen, business, CK7, cytokeratin 7, Non-small-cell lung cancer, Progressive disease, medicine.drug

الوصف: Introduction We report a case of sustained complete response in unfavorable cancer of unknown primary site (CUP) successfully treated with chemotherapy combining pembrolizumab, pemetrexed and platinum. Case presentation A 66-year-old man was presented with weight loss and cough for 3 months. Contrast-enhanced computed tomography (CT) confirmed a mass in the superior anterior mediastinum and multiple enlarged mediastinal and axillary lymph nodes. Positron emission tomography-CT (PET-CT) showed abnormal uptake in the corresponding lesions. Histopathological analysis of the left axillary nodule revealed poorly differentiated adenocarcinoma. Immunohistochemistry showed the tumor cells were positive for cytokeratin 7 and thyroid transcription factor-1 and negative for cytokeratin 20. Thus, the patient was diagnosed as poorly differentiated adenocarcinoma of unknown primary, and treated as non-small-cell lung cancer. Additional genetic testing revealed the patient was negative for EGFR, ALK fluorescence in situ hybridization, ROS1, BRAF, and PD-L1 22C3 IHC with Tumor Proportion Score (TPS) was less than 1%. The patient received six cycles of pembrolizumab, platinum, and pemetrexed intravenously. Cisplatin was switched to carboplatin because of cisplatin nephrotoxicity in one course. PET-CT after six cycles showed all lesions disappeared; complete response was considered to have been achieved. Maintenance therapy of pembrolizumab and pemetrexed has been continued for 6 months after the induction therapies to prevent progressive disease. Complete response has been maintained. Discussion Chemotherapy with pembrolizumab, platinum and pemetrexed could be valuable for treating unfavorable CUP. Conclusion Chemotherapy with pembrolizumab, platinum, and pemetrexed helped achieved sustained complete response in a patient with unfavorable CUP.
Highlights • The prognosis of patients with unfavorable CUP remains poor. • Pembrolizumab's addition to standard chemotherapy resulted in significantly longer overall survival for patients with NSCLC. • Chemotherapy with pembrolizumab, platinum, and pemetrexed helped achieved sustained complete response in a patient with unfavorable CUP.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::931c4a2f9e20fcfc3a4cf8a935b4c559Test
http://europepmc.org/articles/PMC7578551Test

8

Triple 'L' (Leukocytosis, Lactate, and LDH): Predictors of Mesenteric Ischemia

المؤلفون: K. P. Mishra, Sita Ram Gosh, A. K. Singh

المصدر: Indian Journal of Surgery. 83:865-869

الوصف: To investigate the diagnostic value of total leucocyte counts, plasma lactate dehydrogenase (LDH), and serum lactate levels in combination for diagnosing acute mesenteric ischemia (AMI). This cross-sectional observation study included patients presenting to a tertiary care hospital of Indian Armed Forces with acute abdomen and were found to have mesenteric ischemia at laparotomy. A total of thirty cases of acute abdomen found to have mesenteric ischemia at laparotomy were studied over a period from September 2016 to December 2019. Preoperative leukocytosis, serum lactate levels, and LDH levels were observed in the study population and correlated with intraoperative findings to see whether a preoperative raised value were indicators of mesenteric ischemia when used in combination. A total of 30 patients were observed. Twenty-seven patients were male and 3 were females. Diagnosis was verified at laparotomy. It was observed that 43.3% of the patients had all the three variables positive (raised LDH, lactate, and leukocytosis). At least two of the three variables were positive in 83.3% of the patients of mesenteric ischemia. At least one variable was positive in all patients of mesenteric ischemia. The findings indicate that raised plasma LDH levels, leukocytosis, and serum lactate levels are better predictors of mesenteric ischemia if taken in combinations. In a clinical setting where mesenteric ischemia is suspected, raised levels of at least two of the three markers are indicators of mesenteric ischemia in more than 80% of cases. In case all three markers are negative, mesenteric ischemia is unlikely and alternative diagnosis needs consideration.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::c652680be2d8c66db1f3508d23b2c074Test
https://doi.org/10.1007/s12262-020-02495-2Test

9

Study of serum creatine kinase and lactate dehydrogenase levels and their correlation in high-voltage electric contact burns

المؤلفون: Shardendu Sharma, Vaddi Suman Babu, Rajkumar R, Raj Kumar Manas, Charanjeet Kaur

المصدر: European Journal of Plastic Surgery. 44:103-110

الوصف: High-voltage electric contact burns often lead to amputation of limbs. Raised muscle enzymes reflect the extent of muscle damage. We aimed to estimate the levels of creatine kinase (CK-T and CK-MB) and lactate dehydrogenase (LDH) in high-voltage electric burns and to find out the correlation with amputation. A prospective observational study was conducted in 60 patients over a period of 14 months, and they were divided into 2 groups: ‘amputation’ and ‘no amputation.’ Decision regarding amputation was purely based on clinical assessment either early or late (within or after 72 h). CK, CK-MB, and LDH levels were measured for the first 3 days following an injury and compared. Statistical analysis was done using SPSS software. A p–value of

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::d4838e5b25332cb775d37873855ecbb0Test
https://doi.org/10.1007/s00238-020-01695-zTest

10

Clinical and pathological differences between polymorphonuclear-rich and lymphocyte-rich tuberculous pleural effusion

المؤلفون: Yurong Xu, Bing Chen, Yiqing Qu, Tingting Zhao

المصدر: Annals of Thoracic Medicine
Annals of Thoracic Medicine, Vol 15, Iss 2, Pp 76-83 (2020)

مصطلحات موضوعية: Pulmonary and Respiratory Medicine, medicine.medical_specialty, lcsh:Diseases of the circulatory (Cardiovascular) system, Pleural effusion, tuberculous granuloma, medicine.medical_treatment, Lymphocyte, thoracoscopy, Gastroenterology, Mycobacterium tuberculosis, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, tuberculous pleural effusions, Internal medicine, Lactate dehydrogenase, Biopsy, medicine, Thoracoscopy, 030212 general & internal medicine, lcsh:RC705-779, biology, medicine.diagnostic_test, business.industry, lactate dehydrogenase, lcsh:Diseases of the respiratory system, Decortication, biology.organism_classification, medicine.disease, medicine.anatomical_structure, Adenosine deaminase, 030228 respiratory system, chemistry, Effusion, lcsh:RC666-701, Surgery, Original Article, Cardiology and Cardiovascular Medicine, business

الوصف: OBJECTIVE: Analysis of the occurrence factors and disease characteristics of tuberculous (TB) pleural effusion (TPE) dominated by neutrophils. METHODS: We retrospectively analyzed the clinical data of 304 patients with two types of TB pleurisy. The clinical, laboratory, and pathological features of TB pleurisy separately dominated by lymphocytes and neutrophils were analyzed. RESULTS: Neutrophil-predominant effusion was observed in 33 (10.9%) patients. The patients with TPE with polymorphonuclear leukocytes (PMNLs) had higher fever rates and higher decortication rates than those with lymphocyte-predominant TPE. Otherwise, they had lower chest distress rates and lower positive rates of pulmonary TB and lower biopsy tissue culture-positive rates than patients with lymphocyte-predominant TPE. PMNL TPE patients had higher lactic acid dehydrogenase (LDH) (1297 vs. 410 U/l, P < 0.001) and adenosine deaminase (ADA) levels (54.1 vs. 42.9 U/l, P = 0.043) and lower pleural fluid glucose (1.92 vs. 4.70 mmol/L, P < 0.001) and protein (47.4 vs. 48.4 g/L, P = 0.024) levels than that of lymphocyte-predominant TPE. Otherwise, they had lower blood ALB levels and higher C-reactive protein levels than lymphocyte-predominant TPE. Finally, PMNL TPE patients had lower rates of granuloma formation (27.2% vs. 75.2%, P < 0.001) and pleural nodules than patients with lymphocyte-predominant TPE and more frequent findings of pus, caseous exudate, and necrosis. CONCLUSION: The TB pleurisy patients dominated by neutrophils show strong inflammatory reactions and higher ADA levels in pleural effusion. These findings can significantly improve the positive rate of Mycobacterium tuberculosis in neutrophil-predominant TPE under thoracoscopy.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f66b67b37a9f62f1e8450e9768caf086Test
https://pubmed.ncbi.nlm.nih.gov/32489442Test

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