المؤلفون: Stéphane Varin, Grégoire Cormier, Lucie Planche, Benoit Le Goff, Guillaume Coiffier, Jean-David Albert, Christelle Darrieutort-Laffite, Yves Maugars

المصدر: Annals of the Rheumatic Diseases. 78:837-843

مصطلحات موضوعية: Adult, Male, Visual analogue scale, medicine.medical_treatment, Immunology, Equivalence Trials as Topic, Punctures, Methylprednisolone, General Biochemistry, Genetics and Molecular Biology, Injections, Intra-Articular, Rotator Cuff, 03 medical and health sciences, 0302 clinical medicine, Double-Blind Method, Rheumatology, Tendinitis, Shoulder Pain, Humans, Immunology and Allergy, Medicine, Therapeutic Irrigation, Glucocorticoids, Saline, Ultrasonography, Interventional, Pain Measurement, 030203 arthritis & rheumatology, Pain, Postoperative, 030222 orthopedics, Dry needling, business.industry, Calcinosis, Calcific tendinitis, Middle Aged, medicine.disease, Acute Pain, Treatment Outcome, Anesthesia, Tendinopathy, Female, Saline Solution, Subacromial bursa, business, Follow-Up Studies, medicine.drug, Calcification

الوصف: ObjectiveSteroid injections are common after an ultrasound-guided puncture and lavage (UGPL) of calcific tendonitis of the rotator cuff. However, steroids may prevent calcification resorption and negatively affect tendon healing. Our study was designed to determine whether saline solution was non-inferior to steroids in the prevention of acute pain reactions in the week following UGPL.MethodsThis was a randomised, double-blinded, controlled non-inferiority trial with 12-month follow-up. We included 132 patients (66 in each group) with symptomatic calcification measuring more than 5 mm. Patients received 1 mL of saline or steroid (methylprednisolone 40 mg) in the subacromial bursa at the end of UGPL. Primary outcome was the maximal pain during the week following the procedure with a prespecified non-inferiority margin of 10 mm (0–100 visual analogue scale). Secondary outcomes included pain at rest and during activity, function (disabilities of the arm, shoulder and hand score) and radiological evolution of the calcification over the 12-month follow-up.ResultsThe estimated mean difference in the first week’s maximal pain between these two groups was 11.76 (95% CI 3.78 to 19.75). Steroids significantly improved VAS pain at rest and during activities, as well as function at 7 days and 6 weeks. They did not change the rate of calcification resorption, which occurred in 83% and 74% of patients at 12 months in the saline and steroid groups.ConclusionNon-inferiority of saline when compared with steroids could not be established. However, steroid injection improved pain in the 6 weeks following the procedure, and function in the 3 months after, with no significant effect on calcification resorption.Trial registration numberNTC02403856.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dcece3babe2a404951bc62970e939b9fTest
https://doi.org/10.1136/annrheumdis-2018-214971Test

المؤلفون: Catherine Chevalier, Julien Herman, Frédéric Blanchard, Julien De Lima, Christelle Darrieutort-Laffite, Benoit Le Goff, Régis Brion

المصدر: Arthritis Research & Therapy, Vol 23, Iss 1, Pp 1-11 (2021)
Arthritis Research & Therapy

مصطلحات موضوعية: 0301 basic medicine, Rotator cuff tendons, Pathology, medicine.medical_specialty, Myeloid, Inflammasomes, Interleukin-1beta, Inflammation, Diseases of the musculoskeletal system, Inflammasome, 03 medical and health sciences, 0302 clinical medicine, In vivo, Apatites, NLR Family, Pyrin Domain-Containing 3 Protein, medicine, Humans, Apatite, 030203 arthritis & rheumatology, Chemistry, Caspase 1, Air pouch model, Interleukin, medicine.disease, In vitro, 030104 developmental biology, medicine.anatomical_structure, RC925-935, Tendinopathy, Tumor necrosis factor alpha, medicine.symptom, Research Article, Interleukin-1, medicine.drug, Calcification

الوصف: Background Calcific tendonitis of the rotator cuff is due to carbonated apatite deposits in the shoulder tendons. During the evolution of the disease, an acute inflammatory episode may occur leading to the disappearance of the calcification. Although hydroxyapatite crystal-induced inflammation has been previously studied with synthetic crystals, no data are available with calcifications extracted from patients suffering from calcific tendinopathy. The objective of the study was to explore the inflammatory properties of human calcifications and the pathways involved. Methods Human calcifications and synthetic hydroxyapatite were used in vitro to stimulate human monocytes and macrophages, the human myeloid cell line THP-1, and human tenocytes. The release of IL-1β, IL-6, and IL-8 by cells was quantified by ELISA. The gene expression of pro- and anti-inflammatory cytokines was evaluated by quantitative PCR. NF-kB activation and NLRP3 involvement were assessed in THP-1 cells using a NF-kB inhibitor and a caspase-1 inhibitor. The inflammatory properties were then assessed in vivo using a mouse air pouch model. Results Human calcifications were able to induce a significant release of IL-1β when incubated with monocytes, macrophages, and THP-1 only if they were first primed with LPS (monocytes and macrophages) or PMA (THP-1). Stimulation of THP-1 by human calcifications led to similar levels of IL-1β when compared to synthetic hydroxyapatite although these levels were significantly inferior in monocytes and macrophages. The patient’s crystals enhanced mRNA expression of pro-IL-1β, as well as IL-18, NF-kB, and TGFβ when IL-6 and TNFα expression were not. IL-1β production was reduced by the inhibition of caspase-1 indicating the role of NLRP3 inflammasome. In vivo, injection of human calcifications or synthetic hydroxyapatite in the air pouch led to a significant increase in membrane thickness although significant overexpression of IL-1β was only observed for synthetic hydroxyapatite. Conclusions As synthetic hydroxyapatite, human calcifications were able to induce an inflammatory response resulting in the production of IL-1β after NF-kB activation and through NLRP3 inflammasome. In some experiments, IL-1β induction was lower with human calcifications compared to synthetic apatite. Differences in size, shape, and protein content may explain this observation.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a18bc9d94eb8b9d074ab6b6da7d601edTest
https://doi.org/10.21203/rs.3.rs-156807/v1Test

المؤلفون: Benoit Le Goff, Pascale Guillot, Joëlle Glémarec, Yves Maugars, Christelle Darrieutort-Laffite, Jean-Marie Berthelot

المصدر: Journal of Medical Case Reports, Vol 14, Iss 1, Pp 1-7 (2020)
Journal of Medical Case Reports

مصطلحات موضوعية: medicine.medical_specialty, Bone density, Fractures, Multiple, medicine.medical_treatment, Osteoporosis, lcsh:Medicine, Case Report, 030204 cardiovascular system & hematology, Tertiary hyperparathyroidism, Bone remodeling, 03 medical and health sciences, 0302 clinical medicine, Bone Density, medicine, Humans, Child, Bone mineral, Hyperparathyroidism, Hyperplasia, Bone Density Conservation Agents, business.industry, lcsh:R, Thyroidectomy, General Medicine, Middle Aged, medicine.disease, Surgery, Denosumab rebound, Fracture, Denosumab, 030220 oncology & carcinogenesis, Hypercalcemia, Female, business, Follow-Up Studies, medicine.drug

الوصف: Background The rebound effect after stopping treatment with denosumab may be associated with rapid loss of the gains in bone mineral density achieved with treatment, high levels of bone remodeling markers, the occurrence of vertebral fractures, and even hypercalcemia. Case presentation A 64-year-old osteoporotic Caucasian woman suffered from a fracture of her second lumbar vertebra in 2004. From January 2005, she was treated with denosumab for 9 years, with good densitometry results for her hip and lumbar areas, and no fractures over the last 6 years of treatment. Ten months after the treatment with denosumab was stopped, a cascade of vertebral fractures, including some in unusual locations (third thoracic vertebra), and multiple rib fractures in a context of hypercalcemia, suggested possible malignancy. A complete evaluation, including systemic, biological, and biopsy analyses, ruled out this hypothesis. The hypercalcemia was associated with normal plasma phosphate and vitamin D concentrations, and a high parathyroid hormone level, with an abnormal fixation of the lower lobe of the thyroid on sesta-methoxy-isobutyl-isonitrile scintigraphy. Histological analysis of the excised parathyroid tissue revealed hyperplasia. The associated thyroidectomy (goiter) led to the discovery of a thyroid papillary microcarcinoma. Conclusions We consider the consequences of this rebound effect, not only in terms of the major loss of bone density (return to basal values within 3 years) and the multiple disabling fracture episodes, but also in terms of the hypercalcemia observed in association with apparently autonomous tertiary hyperparathyroidism. Several cases of spontaneous reversion have been reported in children, but the intervention in our patient precluded any assessment of the possible natural course. The discovery of an associated thyroid neoplasm appears to be fortuitous. Better understanding of the various presentations of the rebound effect after stopping treatment with denosumab would improve diagnostic management of misleading forms, as in this case. Bisphosphonates could partially prevent this rebound effect.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2515c1e8c006be893b6d353178c6ef94Test
https://doi.org/10.1186/s13256-020-02401-0Test

المؤلفون: Philippe Montigny, Grégoire Cormier, Benoit Le Goff, Céline Cozic, Gilles Tanguy, Christelle Volteau, Yves Maugars, Stéphane Varin, Joëlle Glémarec, Christelle Darrieutort Laffite

المصدر: Joint Bone Spine. 85:359-363

مصطلحات موضوعية: Adult, Male, medicine.medical_specialty, Lidocaine, Visual analogue scale, medicine.drug_class, medicine.medical_treatment, Injections, Intralesional, Cortivazol, Placebo, Risk Assessment, Severity of Illness Index, Statistics, Nonparametric, 03 medical and health sciences, 0302 clinical medicine, Double-Blind Method, Rheumatology, Reference Values, 030202 anesthesiology, medicine, Humans, Prospective Studies, Anesthetics, Local, Glucocorticoids, Saline, Aged, Pain Measurement, Lumbar Vertebrae, Local anesthetic, business.industry, Lumbosacral Region, Middle Aged, Prognosis, Low back pain, Surgery, Treatment Outcome, Anesthesia, Drug Therapy, Combination, Female, medicine.symptom, Tomography, X-Ray Computed, business, Low Back Pain, 030217 neurology & neurosurgery, Lumbosacral joint, medicine.drug

الوصف: Objective The primary objective of this study was to compare the efficacy of local injection of a local anesthetic with a glucocorticoid versus a local anesthetic with saline to treat low back pain due to lumbosacral transitional vertebras (LSTV) with a pseudoarticulation. Methods A randomized placebo-controlled double-blind study was conducted in patients with unilateral low back pain ascribed clinically to LSTV. Patients were randomized to lidocaine plus saline (LS group) or lidocaine plus cortivazol (LC group) injected locally under computed tomography guidance. The primary outcome measure was the 24-hour mean visual analog scale (VAS) score for low back pain 4 weeks after the injection. Results Of 16 randomized patients, 15 were included in the analysis, 8 in the LS group and 7 in the LC group. The mean VAS pain score at week 4 was not significantly different between the two groups. In the two groups pooled, the mean VAS pain score decreased significantly from baseline to week 4, from 5.52 ± 0.99 to 3.86 ± 2.55 (P ≤ 0.05). The difference remained significant at week 12. Significant improvements occurred in the EIFEL disability index and items of the Dallas Pain Questionnaire. No adverse events were recorded. Conclusion In patients with chronic low back pain consistent with a symptomatic LSTV type II or IV in the Castellvi classification, a local injection of lidocaine with or without cortivazol may provide sustained improvements in pain and function. The underlying mechanism is unclear.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a9bfad3bd3d962e0479cdcb9f6bd8f59Test
https://doi.org/10.1016/j.jbspin.2017.05.003Test

المؤلفون: E. Correia, Frédéric Blanchard, Christelle Darrieutort-Laffite, B. Le Goff, Paul Arnolfo

المصدر: Annals of the Rheumatic Diseases. 79:1762.1-1763

مصطلحات موضوعية: medicine.medical_specialty, biology, business.industry, Immunology, Calcific tendinitis, Tendonitis, Periostin, medicine.disease, Placebo, Gastroenterology, General Biochemistry, Genetics and Molecular Biology, Rheumatology, Osteoprotegerin, Methylprednisolone, Internal medicine, biology.protein, Immunology and Allergy, Medicine, Osteopontin, business, Calcification, medicine.drug

الوصف: Background:Calcific tendonitis of the rotator cuff is a frequent cause of chronic shoulder pain. It is due to apatite deposits within the tendons. Little data are currently available about proteins associated to crystals within deposits.Objectives:The aim of the study was to quantify 6 proteins in calcific powders obtained from patients who have undergone an ultrasound-guided percutaneous lavage (UGPL) of their calcification and to look for correlations between their concentration and patient characteristics.Methods:Calcific powders were obtained from patients included in the CALCECHO trial whose main objective was to compare post-procedure pain between two groups: methylprednisolone or placebo injected at the end of the lavage [1]. Based on preliminary proteomic data and literature data, the following proteins have been selected and quantified by ELISA: Pigment-epithelium Derived Factor (PEDF), Osteopontin (OPN), Periostin (POSTN), Activin A (ACT A), Osteoprotegerin (OPG) and Bone Morphogenic Protein-2 (BMP-2). The level of each protein was expressed in µg per pg of the total proteins present in the sample. These proteins have been selected for their link to the mineralization. Correlations between the level of each protein and radiographic and ultrasound appearance of the calcific deposits were sought. We also looked for correlations between level of each protein and duration of pain or response to UGPL (Mann-Whitney test).Results:Sixty-six samples were studied: mean age was 48.9 (+/- 9.7) and 68% were female. Mean duration of shoulder pain was 30 months with a mean VAS pain of 68/100 (+/-14). Mean calcification size was 1.8 cm. Results of ELISA were as follows: mean level of PEDF at 1097 pg/µg, mean level of OPG at 135 pg/µg, mean level of POSTN at 6.9 pg/µg, mean level of ACT A at 19.6 pg/µg and mean level of OPN at 49.6 pg/µg although BMP-2 was undetectable. There was no correlation between level of proteins and the size of the calcification or the duration of pain. There was no difference in protein levels between type A and type B calcifications on radiography (classification of the French Society for Arthroscopy). In contrast, levels of POSTN and OPN were significantly higher in nodular calcifications compared to the homogenous (p=0.003 and p=0.01 respectively) or fragmented types (p=0.03 and p=0.04 respectively). Furthermore, calcifications without acoustic shadowing were enriched in POSTN compared to those with (p=0.04). Finally, the periostin level was significantly higher in calcifications that have responded well to UGPL (p=0.02).Conclusion:In this cohort of patients treated by UGPL, we observed higher levels of POSTN and OPN in the less dense calcifications and POSTN enrichment appeared to be associated with a better response to UGPL. Considering these data, further studies will be necessary to better understand the role of this protein in calcific tendonitis.References:[1]Darrieutort-Laffite C, Varin S, Coiffier G, Albert JD, Planche L, Maugars Y, Cormier G, Le Goff B. Are Corticosteroid Injections Needed After Needling and Lavage of Calcific Tendinitis: A Randomized, Double-Blind, Non-Inferiority Trial. Ann Rheum Dis. 2019 Jun;78(6):837-843.Disclosure of Interests:None declared

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::dd3516f2fe03a39e21da6b7a4781b4d9Test
https://doi.org/10.1136/annrheumdis-2020-eular.2632Test

المؤلفون: Benoit Le Goff, Marguerite Chemel-Mary, Yves Maugars, Géraldine Bart, Pascale Guillot, Christelle Darrieutort-Laffite, Mélanie Gahier-Penhoat, Joëlle Glémarec

المصدر: Calcified tissue international. 102(3)

مصطلحات موضوعية: 0301 basic medicine, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, Osteoporosis, 030209 endocrinology & metabolism, Apoptosis, Osteocytes, Bone resorption, Bone and Bones, Bone remodeling, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, Osteoprotegerin, Internal medicine, medicine, Humans, Orthopedics and Sports Medicine, Aged, 80 and over, business.industry, Wnt signaling pathway, Osteonecrosis, medicine.disease, Spine, 030104 developmental biology, medicine.anatomical_structure, Denosumab, DKK1, Osteocyte, Spinal Fractures, Female, Bone Remodeling, business, medicine.drug

الوصف: We report here a case of multiple vertebral osteonecroses with intrasomatic gaseous dissection (Kummell’s disease) occurring 1 year after the end of a 10-year course of denosumab treatment for osteoporosis without fractures. Histomorphometry and bone remodeling markers revealed major bone resorption and the persistence of an inhibition of bone formation. The presence of multiple empty lacunae in the bone provided evidence for high levels of osteocyte apoptosis. Osteocytes direct bone resorption (via the RANK/RANK-L/osteoprotegerin system) and formation (Wnt system, with SOST and DKK1) pathways. The vertebral osteonecrosis in our case may, therefore, have resulted from osteocyte apoptosis, decompensated by the sudden reactivation of bone remodeling after the cessation of denosumab treatment.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9673c3683a34f11f24d01e7c3d0136f0Test
https://pubmed.ncbi.nlm.nih.gov/29318330Test

المؤلفون: Régis Brion, Mathias Chatelais, Benoit Le Goff, Dominique Heymann, Marie-Astrid Boutet, Christelle Darrieutort-Laffite, Frédéric Blanchard

المصدر: Mediators of Inflammation
Mediators of Inflammation, Vol 2014 (2014)

مصطلحات موضوعية: Macrophage colony-stimulating factor, Article Subject, Cell Survival, CD14, Interleukin-1beta, Immunology, Arthritis, Monocytes, Arthritis, Rheumatoid, lcsh:Pathology, medicine, Humans, Cells, Cultured, Tumor Necrosis Factor-alpha, business.industry, Interleukins, Macrophage Colony-Stimulating Factor, Monocyte, Synovial Membrane, Granulocyte-Macrophage Colony-Stimulating Factor, Cell Biology, Fibroblasts, medicine.disease, medicine.anatomical_structure, Granulocyte macrophage colony-stimulating factor, Culture Media, Conditioned, Rheumatoid arthritis, Tumor necrosis factor alpha, Inflammation Mediators, Synovial membrane, business, lcsh:RB1-214, Research Article, medicine.drug

الوصف: Background. Macrophages and synovial fibroblasts (SF) are two major cells implicated in the pathogenesis of rheumatoid arthritis (RA). SF could be a source of cytokines and growth factors driving macrophages survival and activation. Here, we studied the effect of SF on monocyte viability and phenotype.Methods. SF were isolated from synovial tissue of RA patients and CD14+ cells were isolated from peripheral blood of healthy donors. SF conditioned media were collected after 24 hours of culture with or without stimulation with TNFαor IL-1β. Macrophages polarisation was studied by flow cytometry.Results. Conditioned medium from SF significantly increased monocytes viability by 60% compared to CD14+ cells cultured in medium alone(P<0.001). This effect was enhanced using conditioned media from IL-1βand TNFαstimulated SF. GM-CSF but not M-CSF nor IL34 blocking antibodies was able to significantly decrease monocyte viability by 30% when added to the conditioned media from IL-1βand TNFαstimulated SF(P<0.001). Finally, monocyte cultured in presence of SF conditioned media did not exhibit a specific M1 or M2 phenotype.Conclusion. Overall, rheumatoid arthritis synovial fibroblasts stimulated with proinflammatory cytokines (IL-1βand TNFα) promote monocyte viability via GM-CSF but do not induce a specific macrophage polarization.

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الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::156eefe1b0a7d277da15a6d539740262Test
https://doi.org/10.1155/2014/241840Test

المؤلفون: Claire Le Jeunne, Alain Saraux, Gilles Hayem, Xavier Puéchal, Christelle Darrieutort-Laffite, Vincent André, Véronique Le Guern

المصدر: ResearcherID

مصطلحات موضوعية: Adult, medicine.medical_specialty, Urinary urgency, Cyclophosphamide, Urinary system, Cystitis, Interstitial, Azathioprine, Distension, Gastroenterology, Rheumatology, Internal medicine, medicine, Dysuria, Humans, Aged, business.industry, Interstitial cystitis, Cystoscopy, Middle Aged, medicine.disease, Surgery, Sjogren's Syndrome, Female, medicine.symptom, Sjogren s, business, Tomography, X-Ray Computed, Immunosuppressive Agents, medicine.drug, Follow-Up Studies

الوصف: Objectives To characterize the interstitial cystitis (IC) associated with Sjogren's syndrome (SS). Methods Report of three new cases. Only cases fulfilling the American-European consensus criteria for SS and the European Society for the Study of Interstitial Cystitis criteria with positive histological findings for IC were included. Results Thirteen cases of SS and IC have been reported in women, including the three reported here, with a mean age of 54 years. SS appeared first in 77% ( n = 10) of cases, a mean of 6.6 years before IC. The symptoms of IC included pollakiuria ( n = 11), lower abdominal pain ( n = 8), urinary urgency ( n = 5), painful micturition ( n = 6), hematuria ( n = 3) and dysuria ( n = 3). Urinary dilatation occurred in three cases, leading to acute renal failure in two patients. The diagnosis of IC was confirmed by anatomical evidence of cystitis inflammation on bladder biopsy in all ( n = 13) patients. Treatment was reported for nine patients, seven of whom (78%) received corticosteroid treatment, which was partially or completely effective in six cases. Immunosuppressive treatment was added in three cases (cyclosporine, n = 2; azathioprine, n = 1; cyclophosphamide, n = 1). Local bladder treatments were performed, with hydraulic distension in five cases and DMSO instillation in one patient. A urinary catheter was inserted in the two cases of acute obstructive renal failure. Conclusions Urinary symptoms without infection should lead the physician to consider a diagnosis of IC in SS patients. Urinary dilatation may occur, leading to acute obstructive renal failure. Corticosteroid treatment may be effective and local treatments have been tried.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::edfdca807d466bce2e9d5c6a370be673Test
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000358821600007&KeyUID=WOS:000358821600007Test