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المؤلفون: Parabiaghi, A, Tettamanti, M, D'Avanzo, B, Barbato, A, Aguglia, Eugenio, Bufalino, C, Cannavo', D, Gandolfo, L, Bassi, M, Erlicher, A, Agnetti, G, Breviario, G, Casacchia, M, Pollce, R, Pomero, P, Colotto, A, Manfrinati, S, Cattaneo, Ci, Corrivetti, G, Pinto, G, Ferranini, L, Marcenaro, M, Vaggi, M, Ghio, L, Natta, W, Ferrato, F, Francomano, A, La Placha, M, Mastroeni, A, Rigamonti, D, Groppi, C, Mauri, Mc, De Gaspari IF, Percudanim, Picci, R, Comino, L, Paschetta, E, Pioli, R, Bignotti, S, Smerieri, G, Ghinaglia, Visani, E, Lucattini, A, Caverzasi, E, Colombo, R, Cervetti, A, D'Aloise, A, Parise, Vf, Florio, V, Hadjichristos, A, D'Avamzo, B, Buratti, G, Buratti, L, De Micheli, A, Furlato, K, D'Onofrio, S, Mariannantoni, I, Rapisarda, F, Riccardi, F, Ruberto, A, Ruggirello, I, Santini, I, Trivelli, F, Ullo, A.
المصدر: Acta psychiatrica Scandinavica. 133(1)
مصطلحات موضوعية: Olanzapine, Adult, Male, medicine.medical_specialty, medicine.medical_treatment, Aripiprazole, law.invention, 03 medical and health sciences, Benzodiazepines, 0302 clinical medicine, Randomized controlled trial, law, Internal medicine, Haloperidol, Medicine, Humans, Antipsychotic, Psychiatry, Adverse effect, Metabolic Syndrome, business.industry, Middle Aged, medicine.disease, 030227 psychiatry, Discontinuation, Psychiatry and Mental health, Treatment Outcome, Schizophrenia, Female, business, 030217 neurology & neurosurgery, medicine.drug, Antipsychotic Agents, Follow-Up Studies
الوصف: Objective To determine whether the prescription of aripiprazole, compared with olanzapine and haloperidol, was associated with a lower frequency of metabolic syndrome (MS) and treatment discontinuation at 1 year. Method Patients were randomly assigned to be treated open-label and according to usual clinical practice with either aripiprazole, olanzapine, or haloperidol and followed up for 1 year. Results Three hundred out-patients with persistent schizophrenia were recruited in 35 mental health services. The intention-to-treat (ITT) analysis found no significant differences in the rate of MS between aripiprazole (37%), olanzapine (47%), and haloperidol (42%). Treatment discontinuation for any cause was higher for aripiprazole (52%) than for olanzapine (33%; OR, 0.41; P = 0.004), or haloperidol (37%; OR, 0.51; P = 0.030). No significant difference was found between olanzapine and haloperidol. Time to discontinuation for any cause was longer for olanzapine than for aripiprazole (HR, 0.55; P
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1798662732d7eae5509d46d25c14a81Test
https://pubmed.ncbi.nlm.nih.gov/26252780Test -
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المساهمون: Agnetti, G., Piepoli, M.F., Siniscalchi, G., Nicolini, F.
المصدر: BioMed Research International, Vol 2015 (2015)
BioMed Research Internationalمصطلحات موضوعية: Acute coronary syndrome, medicine.medical_specialty, Cardiotonic Agents, medicine.medical_treatment, lcsh:Medicine, Review Article, Disease, General Biochemistry, Genetics and Molecular Biology, medicine, Animals, Humans, Cardiovascular Surgical Procedure, Intensive care medicine, Heart Failure, Heart transplantation, Evidence-Based Medicine, General Immunology and Microbiology, business.industry, Cardiovascular Surgical Procedures, lcsh:R, General Medicine, medicine.disease, Cardiac surgery, Transplantation, N/A, Heart failure, Heart-Assist Devices, business, Biomarkers
الوصف: Cardiovascular disease is the leading cause of mortality in the US and in westernized countries with ischemic heart disease accounting for the majority of these deaths. Paradoxically, the improvements in the medical and surgical treatments of acute coronary syndrome are leading to an increasing number of "survivors" who are then developing heart failure. Despite considerable advances in its management, the gold standard for the treatment of end-stage heart failure patients remains heart transplantation. Nevertheless, this procedure can be offered only to a small percentage of patients who could benefit from a new heart due to the limited availability of donor organs. The aim of this review is to evaluate the safety and efficacy of innovative approaches in the diagnosis and treatment of patients refractory to standard medical therapy and excluded from cardiac transplantation lists. © 2015 Giulio Agnetti et al.
وصف الملف: ELETTRONICO
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f02dbb817d4e28d7980a94f0ffc6ac63Test
https://doi.org/10.1155/2015/265260Test -
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المؤلفون: Jennifer E. Van Eyk, Giulio Agnetti, Cathrine Husberg
المساهمون: Agnetti G, Husberg C, Van Eyk JE.
المصدر: Circulation Research. 108:512-526
مصطلحات موضوعية: Proteomics, Physiology, Computational biology, ORGANELLE, Biology, Second Messenger Systems, Article, Organelle, Intracellular Communication, medicine, Animals, Humans, Heart Failure, Organelles, medicine.disease, Cell biology, INTERMEDIATE FILAMENTS CYTOSKELETON, Protein regulation, Heart failure, Proteome, Signal transduction, Energy Metabolism, Cardiology and Cardiovascular Medicine, Protein Processing, Post-Translational, Function (biology), Signal Transduction
الوصف: Chronic heart failure is a worldwide cause of mortality and morbidity and is the final outcome of a number of different etiologies. This reflects both the complexity of the disease and our incomplete understanding of its underlying molecular mechanisms. One experimental approach to address this is to study subcellular organelles and how their functions are activated and synchronized under physiological and pathological conditions. In this review, we discuss the application of proteomic technologies to organelles and how this has deepened our perception of the cellular proteome and its alterations with heart failure. The use of proteomics to monitor protein quantity and posttranslational modifications has revealed a highly intricate and sophisticated level of protein regulation. Posttranslational modifications have the potential to regulate organelle function and interplay most likely by targeting both structural and signaling proteins throughout the cell, ultimately coordinating their responses. The potentials and limitations of existing proteomic technologies are also discussed emphasizing that the development of novel methods will enhance our ability to further investigate organelles and decode intracellular communication.
وصف الملف: ELETTRONICO
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c1f72e29e11f179000d71cbca4c96a8Test
https://doi.org/10.1161/circresaha.110.226910Test -
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المؤلفون: Pieter P. de Tombe, David A. Kass, Giulio Agnetti, Jennifer E. Van Eyk, Namthip Witayavanitkul, Jonathan A. Kirk, Wei Dong Gao, Viola Kooij, Richard S. Tunin, Ronald J. Holewinski
المساهمون: Kirk JA, Holewinski RJ, Kooij V, Agnetti G, Tunin RS, Witayavanitkul N, de Tombe PP, Gao WD, Van Eyk J, Kass DA
مصطلحات موضوعية: Sarcomeres, medicine.medical_specialty, Myofilament, genetic structures, medicine.medical_treatment, Heart Ventricles, Cardiac resynchronization therapy, chemistry.chemical_element, Biology, Calcium, Cell Enlargement, In Vitro Techniques, Sarcomere, Glycogen Synthase Kinase 3, Dogs, Myofibrils, Troponin T, Internal medicine, Troponin I, medicine, Myocyte, Animals, cardiovascular diseases, Phosphorylation, Ventricular dyssynchrony, Heart Failure, General Medicine, medicine.disease, Myocardial Contraction, Enzyme Activation, Endocrinology, chemistry, Heart failure, Cardiology, cardiovascular system, CARDIAC RESYNCHRONIZATION THERAPY, Protein Processing, Post-Translational, Research Article, circulatory and respiratory physiology
الوصف: Cardiac resynchronization therapy (CRT), the application of biventricular stimulation to correct discoordinate contraction, is the only heart failure treatment that enhances acute and chronic systolic function, increases cardiac work, and reduces mortality. Resting myocyte function also increases after CRT despite only modest improvement in calcium transients, suggesting that CRT may enhance myofilament calcium responsiveness. To test this hypothesis, we examined adult dogs subjected to tachypacing-induced heart failure for 6 weeks, concurrent with ventricular dyssynchrony (HFdys) or CRT. Myofilament force-calcium relationships were measured in skinned trabeculae and/or myocytes. Compared with control, maximal calcium-activated force and calcium sensitivity declined globally in HFdys; however, CRT restored both. Phosphatase PP1 induced calcium desensitization in control and CRT-treated cells, while HFdys cells were unaffected, implying that CRT enhances myofilament phosphorylation. Proteomics revealed phosphorylation sites on Z-disk and M-band proteins, which were predicted to be targets of glycogen synthase kinase-3beta (GSK-3beta). We found that GSK-3beta was deactivated in HFdys and reactivated by CRT. Mass spectrometry of myofilament proteins from HFdys animals incubated with GSK-3beta confirmed GSK-3beta-dependent phosphorylation at many of the same sites observed with CRT. GSK-3beta restored calcium sensitivity in HFdys, but did not affect control or CRT cells. These data indicate that CRT improves calcium responsiveness of myofilaments following HFdys through GSK-3beta reactivation, identifying a therapeutic approach to enhancing contractile function.
وصف الملف: ELETTRONICO
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::65b1e3df7636fe7bbbadfe2622d58cdeTest
http://hdl.handle.net/11585/265302Test -
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المؤلفون: Lesley A. Kane, Nina Kaludercic, Khalid Chakir, D. Samantapudi, Steven T. Elliott, Yurong Guo, Gordon F. Tomaselli, David A. Kass, Giulio Agnetti, Jennifer E. Van Eyk, Nazareno Paolocci
المساهمون: Agnetti G, Kaludercic N, Kane LA, Elliott ST, Guo Y, Chakir K, Samantapudi D, Paolocci N, Tomaselli GF, Kass DA, Van Eyk JE.
مصطلحات موضوعية: medicine.medical_specialty, Proteome, Heart Ventricles, medicine.medical_treatment, Citric Acid Cycle, Cardiac resynchronization therapy, Mitochondrion, Biology, Mitochondria, Heart, Article, Mitochondrial Proteins, Dogs, Internal medicine, Genetics, medicine, Animals, Electrophoresis, Gel, Two-Dimensional, Amino Acid Sequence, Ventricular dyssynchrony, Mitochondrial protein, Genetics (clinical), Cardiac Pacing, Artificial, medicine.disease, Mitochondrial proteome, ATP Synthetase Complexes, Endocrinology, HEART FAILURE, Heart failure, MITOCONDRIA, Cardiology, PROTEOMICS, Cardiology and Cardiovascular Medicine, Protein Processing, Post-Translational, CARDIAC RESYNCHRONYZATION THERAPY
الوصف: Background— Cardiac resynchronization therapy (CRT) improves chamber mechanoenergetics and morbidity and mortality of patients manifesting heart failure with ventricular dyssynchrony; however, little is known about the molecular changes underlying CRT benefits. We hypothesized that mitochondria may play an important role because of their involvement in energy production. Methods and Results— Mitochondria isolated from the left ventricle in a canine model of dyssynchronous or resynchronized (CRT) heart failure were analyzed by a classical, gel-based, proteomic approach. Two-dimensional gel electrophoresis revealed that 31 mitochondrial proteins where changed when controlling the false discovery rate at 30%. Key enzymes in anaplerotic pathways, such as pyruvate carboxylation and branched-chain amino acid oxidation, were increased. These concerted changes, along with others, suggested that CRT may increase the pool of Krebs cycle intermediates and fuel oxidative phosphorylation. Nearly 50% of observed changes pertained to subunits of the respiratory chain. ATP synthase-β subunit of complex V was less degraded, and its phosphorylation modulated by CRT was associated with increased formation (2-fold, P =0.004) and specific activity (+20%, P =0.05) of the mature complex. The importance of these modifications was supported by coordinated changes in mitochondrial chaperones and proteases. CRT increased the mitochondrial respiratory control index with tightened coupling when isolated mitochondria were reexposed to substrates for both complex I (glutamate and malate) and complex II (succinate), an effect likely related to ATP synthase subunit modifications and complex quantity and activity. Conclusions— CRT potently affects both the mitochondrial proteome and the performance associated with improved cardiac function.
وصف الملف: STAMPA
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f44565c0bb414d78e8b74cfecb97c058Test
http://hdl.handle.net/11585/100419Test
