المؤلفون: Alison Lovegrove, Luzie U Wingen, Amy Plummer, Abigail Wood, Diana Passmore, Ondrej Kosik, Jackie Freeman, Rowan A C Mitchell, Kirsty Hassall, Mehmet Ulker, Karolina Tremmel-Bede, Marianna Rakszegi, Zoltán Bedő, Marie-Reine Perretant, Gilles Charmet, Caroline Pont, Jerome Salse, Michelle Leverington Waite, Simon Orford, Amanda Burridge, Till K Pellny, Peter R Shewry, Simon Griffiths

المصدر: PLoS ONE, Vol 15, Iss 2, p e0227826 (2020)

مصطلحات موضوعية: Medicine, Science

الوصف: Dietary fibre (DF) has multiple health benefits and wheat grains are major sources of DF for human health. However, DF is depleted in white wheat flour which is more widely consumed than wholegrain. The major DF component in white flour is the cell wall polysaccharide arabinoxylan (AX). We have identified the Chinese wheat cultivar Yumai 34 as having unusually high contents of AX in both water-soluble and insoluble forms. We have therefore used populations generated from crosses between Yumai 34 and four other wheat cultivars, three with average contents of AX (Ukrainka, Altigo and Claire) and one also having unusually high AX (Valoris), in order to map QTLs for soluble AX (determined as relative viscosity of aqueous extracts of wholemeal flours) and total AX (determined by enzyme fingerprinting of white flour). A number of QTL were mapped, but most were only detected in one or two crosses. However, all four crosses showed strong QTLs for high RV/total AX on chromosome 1B, with Yumai 34 being the increasing parent, and a KASP marker for the Yumai 34 high AX allele was validated by analysis of high AX lines derived from Yumai 34 but selected by biochemical analysis. A QTL for RV was also mapped on chromosome 6B in Yumai 34 x Valoris, with Valoris being the increasing allele, which is consistent with the observation of transgressive segregation for this population. Association studies in an independent germplasm panel identified marker trait associations for relative viscosity in these same locations while direct selection for fibre content in breeding resulted in high levels of enrichment for the Yumai 34 1B allele. The data therefore indicate that marker-assisted breeding can be used to develop wheat with high AX fibre in white flour.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1932-6203Test

الوصول الحر: https://doaj.org/article/28ccb3210c1344c69c7fffc103ce20f7Test

المؤلفون: Kirsty L. Hassall, Marianna Rakszegi, Till K. Pellny, Michelle Leverington Waite, Alison Lovegrove, Amy Plummer, Abigail J. Wood, Luzie U. Wingen, Ondrej Kosik, Gilles Charmet, Rowan A. C. Mitchell, Peter R. Shewry, Karolina Tremmel-Bede, M.R. Perretant, Caroline Pont, Amanda J. Burridge, Zoltán Bedő, Simon Orford, Jackie Freeman, Simon Griffiths, Mehmet Ülker, Diana Passmore, Jérôme Salse

المساهمون: Rothamsted Research, John Innes Centre [Norwich], Yüzüncü Yıl University, Van, Turkey, Centre for Agricultural Research [Budapest] (ATK), Hungarian Academy of Sciences (MTA), Génétique Diversité et Ecophysiologie des Céréales (GDEC), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Bristol [Bristol], Biotechnology and Biological Sciences Research Council (BBSRC)

المصدر: PLoS ONE
PLoS ONE, Public Library of Science, 2020, 15 (e0227826), ⟨10.1371/journal.pone.0227826⟩
PLoS ONE, 2020, 15 (e0227826), ⟨10.1371/journal.pone.0227826⟩
PLoS ONE, Vol 15, Iss 2, p e0227826 (2020)

مصطلحات موضوعية: 0106 biological sciences, Germplasm, [SDV]Life Sciences [q-bio], Flour, Physical Chemistry, 01 natural sciences, chemistry.chemical_compound, Materials Physics, Arabinoxylan, Medicine and Health Sciences, Food science, Cultivar, Triticum, 2. Zero hunger, 0303 health sciences, education.field_of_study, Multidisciplinary, Viscosity, Physics, Eukaryota, Chromosome Mapping, food and beverages, Genomics, Bread, Plants, Chemistry, Wheat, Physical Sciences, Medicine, Xylans, Research Article, Genetic Markers, Science, Materials Science, Quantitative Trait Loci, Population, Wheat flour, Quantitative trait locus, Biology, Research and Analysis Methods, Chromosomes, Plant, Transgressive segregation, 03 medical and health sciences, Genetics, Genome-Wide Association Studies, Grasses, Molecular Biology Techniques, education, Molecular Biology, Alleles, Crosses, Genetic, Nutrition, 030304 developmental biology, Gene Mapping, fungi, Organisms, Biology and Life Sciences, Computational Biology, Reproducibility of Results, Human Genetics, Genome Analysis, Diet, Chemical Properties, chemistry, Food, Genetic Loci, Genetic marker, Lod Score, Genome-Wide Association Study, 010606 plant biology & botany

الوصف: International audience; Dietary fibre (DF) has multiple health benefits and wheat grains are major sources of DF for human health. However, DF is depleted in white wheat flour which is more widely consumed than wholegrain. The major DF component in white flour is the cell wall polysaccharide arabinoxylan (AX). We have identified the Chinese wheat cultivar Yumai 34 as having unusually high contents of AX in both water-soluble and insoluble forms. We have therefore used populations generated from crosses between Yumai 34 and four other wheat cultivars, three with average contents of AX (Ukrainka, Altigo and Claire) and one also having unusually high AX (Valoris), in order to map QTLs for soluble AX (determined as relative viscosity of aqueous extracts of wholemeal flours) and total AX (determined by enzyme fingerprinting of white flour). A number of QTL were mapped, but most were only detected in one or two crosses. However, all four crosses showed strong QTLs for high RV/total AX on chromosome 1B, with Yumai 34 being the increasing parent, and a KASP marker for the Yumai 34 high AX allele was validated by analysis of high AX lines derived from Yumai 34 but selected by biochemical analysis. A QTL for RV was also mapped on chromosome 6B in Yumai 34 x Valoris, with Valoris being the increasing allele, which is consistent with the observation of transgressive segregation for this population. Association studies in an independent germplasm panel identified marker trait associations for relative viscosity in these same locations while direct selection for fibre content in breeding resulted in high levels of enrichment for the Yumai 34 1B allele. The data therefore indicate that marker-assisted breeding can be used to develop wheat with high AX fibre in white flour.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd8e4e6404d9898acf8db49cef0cbbb2Test
https://avesis.yyu.edu.tr/publication/details/9d0aefa4-c685-4348-8d15-aced3b0f0dc4/oaiTest

المؤلفون: C. N. Law, S. M. Reader, K. A. Cant, A. J. Worland, Ian P. King, Simon Orford, T. E. Miller

المصدر: Euphytica. 89:103-106

مصطلحات موضوعية: Genetics, Monosomy, food and beverages, Aneuploidy, Chromosome, Chromosomal translocation, Plant Science, Horticulture, Biology, medicine.disease, Dwarfing, chemistry.chemical_compound, chemistry, medicine, Poaceae, Agronomy and Crop Science, Gene, Gibberellic acid

الوصف: Wheat varieties tend to be chromosomally unstable producing on average 2–3% of plants with abnormal chromosome numbers. A number of semi dwarf wheat varieties, carrying the gibberellic acid insensitive dwarfing genes Rht1 or Rht2, have been seen to produce distinct tall off types due to reduction in dosage of the chromosome carrying the dwarfing gene. The UK variety ‘Brigand’, carrying Rht2 on chromosome 4D, produced very distinct tall off types when this chromosome was reduced in dosage. The frequency of tall off types was sufficiently high to cause the variety to fail United Kingdom statutory uniformity tests. An attempt to prevent the loss of chromosome 4D was made by constructing translocation chromosomes involving the short arm of chromosome 4D, which carries Rht2, and the long arm of chromosome 4S l from Aegilops sharonensis, which carries a gene(s) conferring preferential transmission. The work in this paper describes the field evaluation of two lines carrying 4DS.4DL-4S l L translocations, and demonstrates their success in preventing spontaneously occurring monosomy of chromosome 4D in semi-dwarf wheats.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::0a275b400ed62e74a1157f7fb9313e37Test
https://doi.org/10.1007/bf00015726Test