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1دورية أكاديمية
المؤلفون: Huanhuan Chen, Qiuying Zhu, Guanghua Lan, Yuhua Ruan, Mei Lin, Shujia Liang, Jianjun Li, Huaxiang Lu, Guangjie Tan, Xianwu Pang, Xianmin Ge, Yunxian Huang, Zhenqiang Chen, Shizhen Zhang, Wenlong Cai
المصدر: BMJ Open, Vol 14, Iss 2 (2024)
مصطلحات موضوعية: Medicine
الوصف: Objectives To evaluate the diagnostic performance of urine HIV antibody rapid test kits in screening diverse populations and to analyse subjects’ willingness regarding reagent types, purchase channels, acceptable prices, and self-testing.Designs Diagnostic accuracy studiesParticipants A total of 2606 valid and eligible samples were collected in the study, including 202 samples from female sex workers (FSWs), 304 persons with injection drug use (IDU), 1000 pregnant women (PW), 100 subjects undergoing voluntary HIV counselling and testing (VCT) and 1000 students in higher education schools or colleges (STUs). Subjects should simultaneously meet the following inclusion criteria: (1) being at least 18 years old and in full civil capacity, (2) signing an informed consent form and (3) providing truthful identifying information to ensure that the subjects and their samples are unique.Results The sensitivity, specificity and area under the curve (AUC) of the urine HIV-1 antibody rapid test kits were 92.16%, 99.92% and 0.960 (95% CI: 0.952 to 0.968, p
وصف الملف: electronic resource
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2دورية أكاديمية
المؤلفون: Yi Chen, Zhiyong Shen, Yi Feng, Yuhua Ruan, Jianjun Li, Shuai Tang, Kailing Tang, Shujia Liang, Xianwu Pang, Edward B. McNeil, Hui Xing, Virasakdi Chongsuvivatwong, Mei Lin, Guanghua Lan
المصدر: Scientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
الوصف: Abstract With the rapid increase in HIV prevalence of men who have sex with men (MSM) in recent years and common human migration and travelling across different provinces in China, MSM are now finding it easier to meet each other, which might contribute to local HIV epidemics as well as fueling cross-province transmission. We performed a cross-sectional survey in 2018–2019 to investigate the current HIV subtype diversity and inferred HIV strain transmission origin among MSM in Guangxi province, China based on a phylogenetic analysis. Based on 238 samples, we found that the HIV-1 subtype diversity was more complicated than before, except for three major HIV subtypes/circulating recombinant forms (CRFs): CRF07_BC, CRF01_AE, CRF55_01B, five other subtypes/CRFs (CRF59_01B, B, CRF08_BC, CRF67_01B, CRF68_01B) and five unique recombinant forms (URFs) were detected. In total, 76.8% (169/220) of samples were infected with HIV from local circulating strains, while others originated from other provinces, predominantly Guangdong and Shanghai. The high diversity of HIV recombinants and complicated HIV transmission sources in Guangxi MSM indicates that there has been an active sexual network between HIV positive MSM both within and outside Guangxi without any effective prevention. Inter-province collaboration must be enforced to provide tailored HIV prevention and control services to MSM in China.
وصف الملف: electronic resource
العلاقة: https://doaj.org/toc/2045-2322Test
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3دورية أكاديمية
المؤلفون: Xianwu Pang, Hui Wei, Jinghua Huang, Qin He, Kailing Tang, Ningye Fang, Xinjuan Zhou, Qiuying Zhu, Xiuling Wu, Guanghua Lan, Zhiyong Shen, Mei Lin, Shujia Liang
المصدر: Scientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
الوصف: Abstract The prevalence of HIV-1 in Guangxi is very high, and the rate of HIV-1 infection among men who have sex with men (MSM) has been increasing. Therefore, it is necessary to explore the patterns and risk factors of HIV transmission in Guangxi. For this purpose, individuals diagnosed with HIV-1 during 2013–2018 in Guangxi were recruited. Phylogenetic relationship, transmission clusters, and genotypic drug resistance analyses were performed based on HIV-1 pol sequences. Related factors were analysed to assess for their association with HIV-1 transmission. CRF07_BC (50.4%) and CRF01_AE (33.4%) were found to be the predominant subtypes. The analysed 1633 sequences (50.15%, Guangxi; 49.85%, other provinces) were segregated into 80 clusters (size per cluster, 2–704). We found that 75.3% of the individuals were in three clusters (size ˃ 100), and 73.8% were high-risk spreaders (links ≥ 4). Infection time, marital status, and subtype were significantly associated with HIV-1 transmission. Additionally, 80.2% of recent infections were linked to long-term infections, and 46.2% were linked to other provinces. A low level of transmitted drug resistance was detected (4.8%). Our findings indicated superclusters and high-risk HIV-1 spreaders among the MSM in Guangxi. Effective strategies blocking the route of transmission should be developed.
وصف الملف: electronic resource
العلاقة: https://doaj.org/toc/2045-2322Test
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4دورية أكاديمية
المؤلفون: Miaomiao Li, Shujia Liang, Chao Zhou, Min Chen, Shu Liang, Chunhua Liu, Zhongbao Zuo, Lei Liu, Yi Feng, Chang Song, Hui Xing, Yuhua Ruan, Yiming Shao, Lingjie Liao
المصدر: Pathogens, Vol 10, Iss 3, p 264 (2021)
مصطلحات موضوعية: HIV drug resistance, sanger sequencing, next-generation sequencing, interrupted antiretroviral therapy, Medicine
الوصف: Patients with antiretroviral therapy interruption have a high risk of virological failure when re-initiating antiretroviral therapy (ART), especially those with HIV drug resistance. Next-generation sequencing may provide close scrutiny on their minority drug resistance variant. A cross-sectional study was conducted in patients with ART interruption in five regions in China in 2016. Through Sanger and next-generation sequencing in parallel, HIV drug resistance was genotyped on their plasma samples. Rates of HIV drug resistance were compared by the McNemar tests. In total, 174 patients were included in this study, with a median 12 (interquartile range (IQR), 6–24) months of ART interruption. Most (86.2%) of them had received efavirenz (EFV)/nevirapine (NVP)-based first-line therapy for a median 16 (IQR, 7–26) months before ART interruption. Sixty-one (35.1%) patients had CRF07_BC HIV-1 strains, 58 (33.3%) CRF08_BC and 35 (20.1%) CRF01_AE. Thirty-four (19.5%) of the 174 patients were detected to harbor HIV drug-resistant variants on Sanger sequencing. Thirty-six (20.7%), 37 (21.3%), 42 (24.1%), 79 (45.4%) and 139 (79.9) patients were identified to have HIV drug resistance by next-generation sequencing at 20% (v.s. Sanger, p = 0.317), 10% (v.s. Sanger, p = 0.180), 5% (v.s. Sanger, p = 0.011), 2% (v.s. Sanger, p < 0.001) and 1% (v.s. Sanger, p < 0.001) of detection thresholds, respectively. K65R was the most common minority mutation, of 95.1% (58/61) and 93.1% (54/58) in CRF07_BC and CRF08_BC, respectively, when compared with 5.7% (2/35) in CRF01_AE (p < 0.001). In 49 patients that followed-up a median 10 months later, HIV drug resistance mutations at >20% frequency such as K103N, M184VI and P225H still existed, but with decreased frequencies. The prevalence of HIV drug resistance in ART interruption was higher than 15% in the survey. Next-generation sequencing was able to detect more minority drug resistance variants than Sanger. There was a sharp increase in minority drug resistance variants when the detection threshold was below 5%.
وصف الملف: electronic resource
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5دورية أكاديمية
المؤلفون: Xiaolin Wang, Xiang He, Ping Zhong, Yongjian Liu, Tao Gui, Dijing Jia, Hanping Li, Jianjun Wu, Jin Yan, Dianmin Kang, Yang Han, Taisheng Li, Rongge Yang, Xiaoxu Han, Lin Chen, Jin Zhao, Hui Xing, Shu Liang, Jianmei He, Yansheng Yan, Yile Xue, Jiafeng Zhang, Xun Zhuang, Shujia Liang, Zuoyi Bao, Tianyi Li, Daomin Zhuang, Siyang Liu, Jingwan Han, Lei Jia, Jingyun Li, Lin Li
المصدر: Scientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
الوصف: Abstract As the most dominant HIV-1 strain in China, CRF01_AE needs to have its evolutionary and demographic history documented. In this study, we provide phylogenetic analysis of all CRF01_AE pol sequences identified in mainland China. CRF01_AE sequences were collected from the Los Alamos HIV Sequence Database and the local Chinese provincial centers of disease control and prevention. Phylogenetic trees were constructed to identify major epidemic clusters. Bayesian coalescent-based method was used to reconstruct the time scale and demographic history. There were 2965 CRF01_AE sequences from 24 Chinese provinces that were collected, and 5 major epidemic clusters containing 85% of the total CRF01_AE sequences were identified. Every cluster contains sequences from more than 10 provinces with 1 or 2 dominant transmission routes. One cluster arose in the 1990s and 4 clusters arose in the 2000s. Cluster I is in the decline stage, while the other clusters are in the stable stage. Obvious lineage can be observed among sequences from the same transmission route but not the same area. Two large clusters in high-level prevalence were found in MSM (Men who have sex with men), which highlighted that more emphasis should be placed on MSM for HIV control in mainland China.
وصف الملف: electronic resource
العلاقة: https://doaj.org/toc/2045-2322Test
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6دورية أكاديمية
المؤلفون: Lin Li, Guoqing Sun, Shujia Liang, Jianjian Li, Tianyi Li, Zhe Wang, Wei Liu, Shaomin Yang, Yongjian Liu, Xiaolin Wang, Jingyun Li
المصدر: PLoS ONE, Vol 8, Iss 10, p e75777 (2013)
الوصف: Yunnan, Guangxi and Henan are the provinces with the most severe HIV epidemic in China, which were also among the first group of areas providing free ART in 2004. However, little comprehensive data are available on prevalence of HIV subtype and baseline drug resistance in drug-naïve populations. In this study, 1746 treatment-naïve HIV-positive individuals were randomly selected from new-reported cases in Henan, Guangxi and Yunnan. Among of them, subtypes and drug resistance of 1159 strains were determined by amplifying and sequencing full-length pol genes. Significantly different distributions of HIV subtypes prevalent in three provinces were identified (P
وصف الملف: electronic resource
العلاقة: http://europepmc.org/articles/PMC3789720?pdf=renderTest; https://doaj.org/toc/1932-6203Test
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7دورية أكاديمية
المؤلفون: Yi Chen, Zhiqiang Cao, Jianjun Li, Jin Chen, Qiuying Zhu, Shujia Liang, Guanghua Lan, Hui Xing, Lingjie Liao, Yi Feng, Yiming Shao, Yuhua Ruan, Huanhuan Chen
مصطلحات موضوعية: Medicine, Biotechnology, Cancer, Infectious Diseases, Virology, Computational Biology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Mathematical Sciences not elsewhere classified, HIV/AIDS, Longitudinal molecular network, Antiretroviral therapy, Generalized estimating equation
الوصف: Supplementary Material 1: Additional file 1. Sensitivity analysis of HIV transmission of molecular linkages between baseline HIV/AIDS cases during 2014–2019 and newly diagnosed cases during 2017–2020 in Qinzhou, Guangxi, respectively.
الإتاحة: https://doi.org/10.6084/m9.figshare.24741809.v1Test
https://figshare.com/articles/journal_contribution/Additional_file_1_of_HIV_transmission_and_associated_factors_under_the_scale-up_of_HIV_antiretroviral_therapy_a_population-based_longitudinal_molecular_network_study/24741809Test -
8دورية أكاديمية
المؤلفون: Kang Li (107509), Huanhuan Chen (706338), Jianjun Li (139837), Yi Feng (29944), Guanghua Lan (570384), Shujia Liang (465986), Meiliang Liu (8902670), Abdur Rashid (508397), Hui Xing (80421), Zhiyong Shen (344456), Yiming Shao (80425)
مصطلحات موضوعية: Medicine, Immunology, Cancer, Science Policy, Infectious Diseases, Virology, Computational Biology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, HIV-1, genetic sub-cluster, antiretroviral therapy, immune reconstruction, coreceptor tropism
الوصف: There are great disparities of the results in immune reconstruction (IR) of the HIV-1 infected patients during combined antiretroviral therapy (cART), due to both host polymorphisms and viral genetic subtypes. Identifying these factors and elucidating their impact on the IR could help to improve the efficacy. To study the factors influencing the IR, we conducted a 15-year retrospective cohort study of HIV-1 infected individuals under cART. The trend of CD4 + count changes was evaluated by the generalized estimating equations. Cox proportional model and propensity score matching were used to identify variables that affect the possibility of achieving IR. The tropism characteristics of virus were compared using the coreceptor binding model. In addition to baseline CD4 + counts and age implications, CRF01_AE cluster 1 was associated with a poorer probability of achieving IR than infection with cluster 2 (aHR, 1.39; 95%CI, 1.02-1.90) and other subtypes (aHR, 1.83; 95%CI, 1.31-2.56). The mean time from cART initiation to achieve IR was much longer in patients infected by CRF01_AE cluster 1 than other subtypes/sub-clusters ( P < 0.001). In-depth analysis indicated that a higher proportion of CXCR4 viruses were found in CRF01_AE clusters 1 and 2 ( P < 0.05), and showed tendency to favour CXCR4 binding to V3 signatures. This study indicated the immune restoration impairment found in patients were associated with HIV-1 CRF01_AE cluster 1, which was attributed to the high proportion of CXCR4-tropic viruses. To improve the effectiveness of cART, more efforts should be made in the early identification of HIV-1 subtype/sub-cluster and monitoring of virus phenotypes.
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9دورية أكاديمية
المؤلفون: Xiaoshan Xu (1925389), Liuhong Luo (11691923), Chang Song (172416), Jianjun Li (139837), Huanhuan Chen (706338), Qiuying Zhu (696562), Guanghua Lan (570384), Shujia Liang (465986), Zhiyong Shen (344456), Zhiqiang Cao (503623), Yi Feng (29944), Lingjie Liao (284976), Hui Xing (80421), Yiming Shao (80425), Yuhua Ruan (80422)
مصطلحات موضوعية: Medicine, Neuroscience, Biotechnology, Cancer, Infectious Diseases, Virology, HIV, Pretreatment drug resistance (PDR), Antiretroviral therapy (ART), Drug resistance mutations (DRMs), Genetic transmission networks
الوصف: Additional file 1. Changes in pretreatment HIV drug resistance among HIV-positive individuals in southwestern China, 2014–2020.
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10دورية أكاديمية
المؤلفون: Xiaoshan Xu (1925389), Liuhong Luo (11691923), Chang Song (172416), Jianjun Li (139837), Huanhuan Chen (706338), Qiuying Zhu (696562), Guanghua Lan (570384), Shujia Liang (465986), Zhiyong Shen (344456), Zhiqiang Cao (503623), Yi Feng (29944), Lingjie Liao (284976), Hui Xing (80421), Yiming Shao (80425), Yuhua Ruan (80422)
مصطلحات موضوعية: Medicine, Neuroscience, Biotechnology, Cancer, Infectious Diseases, Virology, HIV, Pretreatment drug resistance (PDR), Antiretroviral therapy (ART), Drug resistance mutations (DRMs), Genetic transmission networks
الوصف: Additional file 3. Difference in proportion of HIV-positive individuals belonging to genetic transmission networks who did and did not harbor PDR.
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