Investigation of the In Vivo Metabolism of Sibirioside A and Angoroside C in Rats by HPLC-ESI-IT-TOF-MSn
| العنوان: | Investigation of the In Vivo Metabolism of Sibirioside A and Angoroside C in Rats by HPLC-ESI-IT-TOF-MSn |
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| المؤلفون: | Shao-Qing Cai, Feng Xu, Ming-Ying Shang, Guang-Xue Liu, Li-Jia Liu, Yi-Fan Zhang |
| المصدر: | Molecules, Vol 23, Iss 10, p 2702 (2018) Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry Molecules Volume 23 Issue 10 |
| بيانات النشر: | MDPI AG, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Scrophularia, Spectrometry, Mass, Electrospray Ionization, Coumaric Acids, Electrospray ionization, HPLC-IT-TOF-MSn, Pharmaceutical Science, Plant Roots, 030226 pharmacology & pharmacy, High-performance liquid chromatography, Article, Analytical Chemistry, Rats, Sprague-Dawley, lcsh:QD241-441, Hydroxylation, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Sulfation, lcsh:Organic chemistry, In vivo, sibirioside A, Drug Discovery, medicine, Animals, Humans, Tissue Distribution, Glycosides, Medicine, Chinese Traditional, Physical and Theoretical Chemistry, Chromatography, High Pressure Liquid, Kidney, Scrophulariae Radix, Phenylpropionates, Organic Chemistry, Metabolism, Small intestine, Rats, 030104 developmental biology, medicine.anatomical_structure, chemistry, Biochemistry, Chemistry (miscellaneous), angoroside C, Molecular Medicine, Trisaccharides, metabolism, Drugs, Chinese Herbal |
| الوصف: | Sibirioside A and angoroside C are two important phenylpropanoid glycosides of the traditional Chinese medicine Scrophulariae Radix. High performance liquid chromatography, coupled with an ion trap time-of-flight multistage mass spectrometry equipped with electrospray ionization source (HPLC-ESI-IT-TOF-MSn), was applied to the profile and we identified the metabolites of sibirioside A and angoroside C in vivo in rats. A total of four metabolites of sibirioside A were identified: SM1, SM2 and SM3 which were known as new compounds. A total of 25 metabolites were detected for angoroside C: AM4, AM5, AM6, AM7, AM16, AM17, AM20, AM21, AM22, AM23 and AM25 which were identified to be new compounds. The main metabolic reactions were hydrolysis, reduction, hydroxylation, methylation, sulfation, and gluconylation. The prototype of sibirioside A was widely distributed in tissues found in the heart, liver, spleen, lung, kidney, stomach and small intestine of rats, and mainly distributed in the stomach, small intestine, kidney and liver. But for angoroside C, nothing was found in the viscera except the stomach and small intestine. The metabolites of sibirioside A were mainly eliminated from feces, while it was urine for the metabolites of angoroside C. Furthermore, 19 metabolites were likely to have bioactivities based on the &lsquo PharmMapper&rsquo analysis, which roughly matched the known pharmacological activities of Scrophulariae Radix (SR) and the prototypes. One of the main pharmacological activities of SR in traditional Chinese medicine is anti-diabetes, and the predicted results showed that SM1, SM2, SM3, AM2, AM4, AM5, AM6, AM9, AM10, AM11, AM12, AM13, AM15, AM18, AM19, AM24, and AM25 might be used to cure diabetes. These findings provide a reference for studying the metabolism, distribution and pharmacological actions of phenylpropanoid glycosides in vivo. |
| وصف الملف: | application/pdf |
| تدمد: | 1420-3049 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df91d5137adfb99316808a1f2aa0144cTest https://doi.org/10.3390/molecules23102702Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....df91d5137adfb99316808a1f2aa0144c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14203049 |
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