دورية أكاديمية
In vitro antiviral activity of a double-stranded RNA sodium salt-based medicinal product against SARS-CoV-2
| العنوان: | In vitro antiviral activity of a double-stranded RNA sodium salt-based medicinal product against SARS-CoV-2 |
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| المؤلفون: | G. M. Ignatyev, E. Yu. Shustova, E. A. Rogozhina, P. A. Belyi, K. Ya. Zaslavskaya, V. A. Merkulov |
| المصدر: | Биопрепараты: Профилактика, диагностика, лечение, Vol 23, Iss 3, Pp 290-299 (2023) |
| بيانات النشر: | Ministry of Health of the Russian Federation. Federal State Budgetary Institution «Scientific Centre for Expert Evaluation of Medicinal Products», 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biotechnology LCC:Medicine |
| مصطلحات موضوعية: | double-stranded rna sodium salt, sars-cov-2, covid-19, antiviral activity, interferon-α, interferon-γ, innate immunity, in vitro, vero cells, radamin®viro, Biotechnology, TP248.13-248.65, Medicine |
| الوصف: | Scientific relevance. Innate immune activation in the early phases of COVID-19 infection and subsequent interferon induction may help control viral replication and protect cells not yet infected with SARS-CoV-2. Thus, immunostimulants that induce interferon (IFN), including double-stranded RNA-based agents, are a promising means of post-exposure prophylaxis and treatment of COVID-19 at early stages.Aim. The study evaluated the in vitro antiviral activity of a double-stranded RNA sodium salt-based medicinal product against SARS-CoV-2.Materials and methods. The authors analysed the double-stranded RNA sodium salt-based medicinal product RADAMIN®VIRO using Vero cells and the Delta variant of SARS-CoV-2 (B.1.617). The virus titre was calculated as the tissue cytopathic dose that caused 50% cell death. The authors measured the content of IFN-α and IFN-γ in the culture fluid by enzyme immunoassay and assessed the viral load by real-time polymerase chain reaction (using the cycle threshold value) and by titration (using Vero cells).Results. The studied double-stranded RNA sodium salt-based medicinal product at a concentration of 250 or 500 μg/mL induced IFN-α and IFN-γ expression by Vero cells, thus increasing their resistance to SARS-CoV-2. The authors evaluated the antiviral activity of the medicinal product based on the virus titre, viral load, and cell monolayer damage. The antiviral activity became clear 24 h after treatment, which confirmed the ability of the medicinal product to inhibit the replication of the SARS-CoV-2 virus in vitro as early as the first day after infection.Conclusions. The double-stranded RNA sodium salt-based medicinal product induced IFN-α and IFN-γ synthesis in Vero cells, increasing their resistance to SARS-CoV-2 infection in vitro. These results demonstrate the immunomodulatory and antiviral potential of the medicinal product. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | Russian |
| تدمد: | 2221-996X 2619-1156 |
| العلاقة: | https://www.biopreparations.ru/jour/article/view/453Test; https://doaj.org/toc/2221-996XTest; https://doaj.org/toc/2619-1156Test |
| DOI: | 10.30895/2221-996X-2023-23-3-290-299 |
| الوصول الحر: | https://doaj.org/article/404627f0588842359e2ffef0a0ecda6fTest |
| رقم الانضمام: | edsdoj.404627f0588842359e2ffef0a0ecda6f |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 2221996X 26191156 |
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| DOI: | 10.30895/2221-996X-2023-23-3-290-299 |