المؤلفون: Yves Maugars, Pascale Guillot, Joëlle Glémarec, Jean-Marie Berthelot, Benoit Le Goff, Christelle Darrieutort-Laffite

المصدر: Journal of Medical Case Reports, Vol 14, Iss 1, Pp 1-7 (2020)

مصطلحات موضوعية: Osteoporosis, Denosumab rebound, Fracture, Hypercalcemia, Hyperparathyroidism, Medicine

الوصف: Abstract Background The rebound effect after stopping treatment with denosumab may be associated with rapid loss of the gains in bone mineral density achieved with treatment, high levels of bone remodeling markers, the occurrence of vertebral fractures, and even hypercalcemia. Case presentation A 64-year-old osteoporotic Caucasian woman suffered from a fracture of her second lumbar vertebra in 2004. From January 2005, she was treated with denosumab for 9 years, with good densitometry results for her hip and lumbar areas, and no fractures over the last 6 years of treatment. Ten months after the treatment with denosumab was stopped, a cascade of vertebral fractures, including some in unusual locations (third thoracic vertebra), and multiple rib fractures in a context of hypercalcemia, suggested possible malignancy. A complete evaluation, including systemic, biological, and biopsy analyses, ruled out this hypothesis. The hypercalcemia was associated with normal plasma phosphate and vitamin D concentrations, and a high parathyroid hormone level, with an abnormal fixation of the lower lobe of the thyroid on sesta-methoxy-isobutyl-isonitrile scintigraphy. Histological analysis of the excised parathyroid tissue revealed hyperplasia. The associated thyroidectomy (goiter) led to the discovery of a thyroid papillary microcarcinoma. Conclusions We consider the consequences of this rebound effect, not only in terms of the major loss of bone density (return to basal values within 3 years) and the multiple disabling fracture episodes, but also in terms of the hypercalcemia observed in association with apparently autonomous tertiary hyperparathyroidism. Several cases of spontaneous reversion have been reported in children, but the intervention in our patient precluded any assessment of the possible natural course. The discovery of an associated thyroid neoplasm appears to be fortuitous. Better understanding of the various presentations of the rebound effect after stopping treatment with denosumab would improve diagnostic management of misleading forms, as in this case. Bisphosphonates could partially prevent this rebound effect.

وصف الملف: electronic resource

العلاقة: http://link.springer.com/article/10.1186/s13256-020-02401-0Test; https://doaj.org/toc/1752-1947Test

الوصول الحر: https://doaj.org/article/2aa7eb9965a0448c98dcb1d8a7eb6751Test

المؤلفون: Christelle Darrieutort-Laffite, Paul Arnolfo, Thomas Garraud, Annie Adrait, Yohann Couté, Guy Louarn, Valérie Trichet, Pierre Layrolle, Benoit Le Goff, Frédéric Blanchard

المصدر: Journal of Clinical Medicine, Vol 8, Iss 10, p 1544 (2019)

مصطلحات موضوعية: calcific tendonitis, rotator cuff, apatite, hypertrophic chondrocytes, tissue non-specific alkaline phosphatase, Medicine

الوصف: Calcific tendonitis is a frequent cause of chronic shoulder pain. Its cause is currently poorly known. The objectives of this study were to better characterize the cells and mechanisms involved in depositing apatite crystals in human tendons. Histologic sections of cadaveric calcified tendons were analyzed, and human calcific deposits from patients undergoing lavage of their calcification were obtained to perform infrared spectroscopy and mass spectrometry-based proteomic characterizations. In vitro, the mineralization ability of human rotator cuff cells from osteoarthritis donors was assessed by alizarin red or Von Kossa staining. Calcifications were amorphous areas surrounded by a fibrocartilaginous metaplasia containing hypertrophic chondrocyte-like cells that expressed tissue non-specific alkaline phosphatase (TNAP) and ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), which are two key enzymes of the mineralization process. Calcific deposits were composed of apatite crystals associated with proteins involved in bone and cartilage development and endochondral bone growth. In vitro, tenocyte-like cells extracted from the rotator cuff were able to mineralize in osteogenic cultures, and expressed TNAP, type X COLLAGEN, and MMP13, which are hypertrophic chondrocytes markers. The use of a TNAP inhibitor significantly prevented mineral deposits. We provide evidence that tenocytes have a propensity to differentiate into hypertrophic chondrocyte-like cells to produce TNAP-dependent calcium deposits. We believe that these results may pave the way to identifying regulating factors that might represent valuable targets in calcific tendonitis.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2077-0383/8/10/1544Test; https://doaj.org/toc/2077-0383Test

الوصول الحر: https://doaj.org/article/4a09b66646784ec894c792bfb5b3e3d4Test

المؤلفون: Julien Herman (10727197), Benoit Le Goff (9658398), Julien De Lima (8640990), Régis Brion (530868), Catherine Chevalier (197486), Frédéric Blanchard (9511728), Christelle Darrieutort-Laffite (9658389)

مصطلحات موضوعية: Biochemistry, Medicine, Microbiology, Cell Biology, Molecular Biology, Physiology, Immunology, Developmental Biology, Cancer, Infectious Diseases, Space Science, Chemical Sciences not elsewhere classified, Apatite, Rotator cuff tendons, Interleukin-1, Inflammasome, Air pouch model

الوصف: Additional file 1: Supplementary Figure S1. IL-1β release after 6h stimulation of CD14+ monocytes, MCSF-Macrophages, GM-CSF/IFNγ-Macrophages by crystals at 250, 500 and 1000 ng/ml in presence of LPS (lipopolysaccharide) and parallel cell death rate. sHA= synthetic hydroxyapatite, CAL = human calcification CT= control group without crystal; LPS= lipopolysaccharide. n = 3. * p≤ 0,05. Supplementary Figure S2. Western Blot raw images of sHA (positive control) and three patients. Cleaved IL-1β was studied in cellular lysates (CL) and in supernatants (SN). iNF-κB= NF-κB inhibitor; iCasp1= caspase-1 inhibitor; inh= inhibitor; sHA= synthetic hydroxyapatite; CAL= human calcification; CT = control condition without crystals. Supplementary Table 1. Primers used for quantitative Polymerase Chain Reaction.

العلاقة: https://figshare.com/articles/journal_contribution/Additional_file_1_of_Pro-inflammatory_effects_of_human_apatite_crystals_extracted_from_patients_suffering_from_calcific_tendinopathy/14516698Test

الإتاحة: https://doi.org/10.6084/m9.figshare.14516698.v1Test

المؤلفون: Benoit Le Goff, Jean-Marie Berthelot, Christelle Darrieutort-Laffite

المصدر: Revue du Rhumatisme. 89:107-109

مصطلحات موضوعية: Rheumatology, business.industry, Medicine, business

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::afc7f19b63a53cab31167b47acb67befTest
https://doi.org/10.1016/j.rhum.2020.11.007Test

المؤلفون: Géraldine Bart, Benoit Le Goff, Laurie Charret, Grégoire Cormier, David Boutoille, Emmanuelle Dernis, Christelle Darrieutort-Laffite, Emmanuel Hoppe

المصدر: Journal of Antimicrobial Chemotherapy. 76:3029-3032

مصطلحات موضوعية: Male, 0301 basic medicine, Microbiology (medical), medicine.medical_specialty, Bursitis, medicine.drug_class, Olecranon, 030106 microbiology, Antibiotics, medicine.disease_cause, Septic bursitis, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, Antibiotic therapy, Elbow Joint, medicine, Humans, Pharmacology (medical), Olecranon Process, Pharmacology, 030222 orthopedics, Streptococcus, business.industry, Bacterial Infections, Middle Aged, medicine.disease, Comorbidity, Anti-Bacterial Agents, Infectious Diseases, medicine.anatomical_structure, Cellulitis, Female, business

الوصف: Background No current guidelines are available for managing septic bursitis (SB). Objectives To describe the clinical characteristics and management of olecranon and prepatellar SB in five French tertiary care centres. Methods This is a retrospective observational multicentre study. SB was diagnosed on the basis of positive cultures of bursal aspirate. In the absence of positive bursal fluid, the diagnosis came from typical clinical presentation, exclusion of other causes of bursitis and favourable response to antibiotic therapy. Results We included 272 patients (median age of 53 years, 85.3% male and 22.8% with at least one comorbidity). A microorganism was identified in 184 patients (67.6%), from bursal fluids in all but 4. We identified staphylococci in 135 samples (73.4%), streptococci in 35 (19%) and 10 (5.5%) were polymicrobial, while 43/223 bursal samples remained sterile (19.3%). Forty-nine patients (18%) were managed without bursal fluid analysis. Antibiotic treatment was initially administered IV in 41% and this route was preferred in case of fever (P = 0.003) or extensive cellulitis (P = 0.002). Seventy-one (26%) patients were treated surgically. A low failure rate was observed (n = 16/272, 5.9%) and failures were more frequent when the antibiotic therapy lasted Conclusions Despite variable treatments, SB resolved in the majority of cases even when the treatment was exclusively medical. The success rate was equivalent in the non-surgical and the surgical management groups. However, a treatment duration of

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dafaeae5cf13b4a9e6db087760bf84c9Test
https://doi.org/10.1093/jac/dkab265Test

المؤلفون: Robert Touitou, Géraldine Bart, Marion Couderc, E. Veillard, Pascal Guggenbuhl, P. Coquerelle, Sophie Godot, Christian Lormeau, Denis Mulleman, Guillaume Coiffier, Carine Salliot, Marion Baldeyrou, Beate Hyem, Jean-Marc Ziza, Thomas W. Bauer, René-Marc Flipo, Christelle Darrieutort-Laffite, Raphaèle Seror, Bernard Fouquet, Louis Bernard

المساهمون: Imagerie Moléculaire et Stratégies Théranostiques (IMoST), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA), CHU Gabriel Montpied [Clermont-Ferrand], CHU Clermont-Ferrand

المصدر: Joint Bone Spine
Joint Bone Spine, 2020, 87 (6), pp.538-547. ⟨10.1016/j.jbspin.2020.07.012⟩
Joint Bone Spine, Elsevier Masson, 2020, 87 (6), pp.538-547. ⟨10.1016/j.jbspin.2020.07.012⟩

مصطلحات موضوعية: Adult, Staphylococcus aureus, medicine.medical_specialty, medicine.drug_class, [SDV]Life Sciences [q-bio], Antibiotics, 03 medical and health sciences, 0302 clinical medicine, Rheumatology, Internal medicine, Humans, Medicine, Streptococcus gallolyticus, 030212 general & internal medicine, Medical diagnosis, Intensive care medicine, ComputingMilieux_MISCELLANEOUS, Language, 030203 arthritis & rheumatology, Arthritis, Infectious, business.industry, Staphylococcal Infections, medicine.disease, Anti-Bacterial Agents, 3. Good health, Joint aspiration, Infective endocarditis, Septic arthritis, business, Trauma surgery

الوصف: Septic arthritis (SA) in an adult native joint is a rare condition but a diagnostic emergency due to the morbidity and mortality and the functional risk related to structural damage. Current management varies and the recommendations available are dated. The French Rheumatology Society (SFR) Bone and Joint Infection Working Group, together with the French Language Infectious Diseases Society (SPILF) and the French Orthopaedic and Trauma Surgery Society (SOFCOT) have worked according to the HAS methodology to devise clinical practice recommendations to diagnose and treat SA in an adult native joint. One new focus is on the importance of microbiological documentation (blood cultures and joint aspiration) before starting antibiotic treatment, looking for differential diagnoses (microcrystal detection), the relevance of a joint ultrasound to guide aspiration, and the indication to perform a reference X-ray. A cardiac ultrasound is indicated only in cases of SA involving Staphylococcus aureus, oral streptococci, Streptococcus gallolyticus or Enterococcus faecalis, or when infective endocarditis is clinically suspected. Regarding treatment, we stress the importance of medical and surgical collaboration. Antibiotic therapies (drugs and durations) are presented in the form of didactic tables according to the main bacteria in question (staphylococci, streptococci and gram-negative rods). Probabilistic antibiotic therapy should only be used for patients with serious symptoms. Lastly, non-drug treatments such as joint drainage and early physical therapy are the subject of specific recommendations.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e9308e225fae67b0cbfb68fa506305f5Test
https://doi.org/10.1016/j.jbspin.2020.07.012Test

المؤلفون: Claire Bernier, Hélène Aubert, Jean-Marie Berthelot, Antoine Néel, Christian Agard, Sébastien Barbarot, Benoit Le Goff, Mohamed Hamidou, Agathe Masseau, Françoise Kraeber-Bodéré, Christelle Darrieutort-Laffite, Catherine Ansquer

المصدر: Arthritis Research & Therapy, Vol 22, Iss 1, Pp 1-10 (2020)
Arthritis Research & Therapy

مصطلحات موضوعية: Male, medicine.medical_specialty, lcsh:Diseases of the musculoskeletal system, Interleukin 1 receptor antagonist, Single Center, Cohort Studies, 030207 dermatology & venereal diseases, 03 medical and health sciences, 0302 clinical medicine, Schnitzler syndrome, Musculoskeletal Pain, Internal medicine, medicine, Humans, Bone pain, Retrospective Studies, business.industry, Waldenstrom macroglobulinemia, Retrospective cohort study, Bone scan, medicine.disease, Rash, Rheumatology, Bone lesions, Cohort, Female, medicine.symptom, lcsh:RC925-935, business, 030215 immunology, Research Article, Follow-Up Studies

الوصف: Objective To report on the characteristics and long-term course of rheumatic manifestations in Schnitzler syndrome (SchS). Methods A retrospective cohort study of patients with SchS followed between 2000 and 2020. Inclusion criteria included a diagnosis of SchS (Strasbourg criteria). All available bone scans were reviewed and scored according to the intensity and number of pathological sites. The scintigraphic score was compared with the clinical activity score, CRP level, and treatments. Results Twenty-five patients were included. Median age at diagnosis was 68 years. Eighty patients (72%) had SchS-related rheumatic pain. Most patients had a long-standing isolated rash before constitutional and/or rheumatic symptoms appeared. The monoclonal component level was usually very low (IgMκ in 22/25). Rheumatic pain predominated around the knees. Bone scans revealed abnormal tracer uptake in 15/18 (85%). The scintigraphic score correlated with clinical activity (r = 0.4, p r = 0.47, p p Conclusions Rheumatic manifestations are very prevalent in SchS. However, bone pain can be misleading and contribute to misdiagnosis. Bone scan abnormalities are very prevalent and correlate with disease activity and treatments. IL1-Ra has a dramatic and durable efficacy but may not be required in every patient early on.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52bd4540be4dcd843b01445f9f1276c5Test
http://link.springer.com/article/10.1186/s13075-020-02318-5Test

المؤلفون: Stéphane Ploteau, Benoit Le Goff, Jean-Marie Berthelot, Christelle Darrieutort-Laffite, Frédéric Douane

المصدر: Joint bone spine. 89(2)

مصطلحات موضوعية: business.industry, Spinal stenosis, Hyperemia, Anatomy, medicine.disease, Thrombosis, Venous stasis, medicine.anatomical_structure, Rheumatology, Pregnancy, Tissue Plasminogen Activator, Vasa nervorum, medicine, Humans, Female, Arachnoiditis, Radiculopathies, business, Varices, Vein, Radiculopathy, Spinal Nerve Roots, Intervertebral Disc Displacement

الوصف: Compression of roots/nerves can disrupt some of their functions, but does not necessarily cause pain. This is illustrated by the frequency of nearly asymptomatic spinal stenosis or disc herniations. In fact, pain of radiculopathies (and nerve entrapments) may mostly be the consequence of intraneural oedema induced by microscopical venous stasis around roots/spinal ganglia (or nerves) not or poorly shown by imaging. This narrative review first lists arguments for a role of congestion of vasa-nervorum in the pathophysiology of radiculopathies, including those induced by disc herniation and spinal stenosis, but also other sources of overpressures in spinal venous plexuses (pregnancy, vena cava atresia and thrombosis, portal hypertension, epidural varices, arterio-venous fistula, vertebral hemangioma or hemangioblastoma). It also details sources of venous congestion around nerves outside the spine, from pelvis (May-Thurner syndrome, Nut-cracker syndrome) to buttocks (superior and inferior gluteal veins), and even thighs and legs. A better recognition of a preeminent role of venous congestion in radiculopathies, plexopathies, and nerve entrapments, should have major consequences: (i) discard the dogma that compression is mandatory to induce root/nerve suffering, since root/nerve adherences in two locations can impair blood flow in vasa-nervorum through root/nerve stretching; (ii) implementation of sensitive techniques to visualise impingement of blood flow around or within roots and nerves; (iii) better prevention of roots/nerves adherence, or arachnoiditis induced by extravascular fibrin deposition secondary to venous stasis.; (iv) optimizing treatments dampening clot formation and/or extravascular fibrin leakage in the intradural/peridural spaces, or around roots/nerves, like guided injection of tissue plasminogen activator.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::882023f8cc3172e7a7bb0c801c413ab5Test
https://pubmed.ncbi.nlm.nih.gov/34653602Test

المؤلفون: Jean-Marie Berthelot, Yves Maugars, Christelle Darrieutort-Laffite, Benoit Le Goff

المصدر: Frontiers in Medicine
Frontiers in Medicine, Vol 8 (2021)

مصطلحات موضوعية: medicine.medical_specialty, Medicine (General), diagnosis, Context (language use), Stimulus (physiology), psyche, 03 medical and health sciences, 0302 clinical medicine, Physical medicine and rehabilitation, R5-920, hypersensitisation, Fibromyalgia, medicine, Chronic fatigue syndrome, pain, Restless legs syndrome, 030203 arthritis & rheumatology, business.industry, Cognitive disorder, Chronic pain, Chronic fatigue, General Medicine, medicine.disease, neuro-imaging, Perspective, Medicine, fibromyalgia, fatigue, business, 030217 neurology & neurosurgery

الوصف: The concept of fibromyalgia has progressed to achieve a certain consensus regarding the definition of the condition. We summarize what is known in 2020, be it in terms of diagnosis, with the criteria that have changed over the years, or at the level of the psychological profile, via the notions of “catastrophizing” and “coping” and post-traumatic syndrome. The importance of fatigue and sleep disorders is underlined, with the chronological sequence of post-traumatic syndrome, chronic fatigue, and then amplification of the pain and the onset of multiple associated symptoms. The etiopathogenic debate has been enriched thanks to neuro-imaging data to discover the start of the central neurological signature. The many associated symptoms are reanalyzed in the context of so-called sister conditions which form sometimes more or less separate entities, such as chronic fatigue syndrome or restless legs syndrome for example. What these conditions have in common is hypersensitivity, not just to pain, but also to all exteroceptive stimuli, from deep sensitivity in the neuro-vegetative system, the sense organs and certain functions of the central nervous system, to the psychological aspects and sleep control. In summary, it is possible to define fibromyalgia as a cognitive disorder of cortical integration of chronic pain, with amplification of painful and sensory nociception, decrease in the threshold for the perception of pain, and persistence of a stimulus that maintains the process in chronicity. Fibromyalgia is part of a group of chronic hypersensitivity syndromes of central origin, with a very wide range of means of expression.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04cf44b17490b63a226cb30e3f2278eeTest
http://europepmc.org/articles/PMC8316633Test

المؤلفون: Caroline Trang, Jean-Marie Berthelot, Yves Maugars, Christelle Darrieutort-Laffite, Benoit Le Goff

المصدر: Joint bone spine. 88(6)

مصطلحات موضوعية: Mycobiota, Innate immune system, biology, business.industry, Microbiota, Fungi, Chromosomal translocation, biology.organism_classification, medicine.disease, Fungal antigen, Microbiology, Gastrointestinal Microbiome, Pathogenesis, CARD Signaling Adaptor Proteins, Mice, Rheumatology, Antigen, Spondylarthritis, Medicine, Animals, Dysbiosis, Humans, business, Bacteria

الوصف: This review lists current evidences for a contribution of gut mycobiota to the pathogenesis of SpA and related conditions. Gut mycobiota has a small size as compared to bacterial microbiota, but an even greater inter- and intra-individual variability. Although most fungi (brought by food or air) are only transitory present, a core mycobiota of gut resident fungi exists, and interplays with bacteria in a complex manner. A dysbiosis of this gut mycobiota has been observed in Crohn's disease and sclerosing cholangitis, with decreased proportion of Saccharomyces cerevisiae and outgrowth of more pathogenic gut fungi. Fungal-induced lower number of commensal gut bacteria can promote translocation of some bacterial/fungal antigens through mucosae, and live fungi can also cross the epithelial border in Crohn's disease. This dysbiosis also lower the ability of bacteria to metabolize tryptophan into regulatory metabolites, consequently enhancing tryptophan metabolism within human cells, which might contribute to fatigue. Translocation of mycobiotal antigens like curdlan (beta-glucan), which plays a major role in the pathogenesis of SpA in the SGK mice, has been observed in humans. This translocation of fungal antigens in human SpA might account for the anti-Saccharomyces antibodies found in this setting. Contribution of fungal antigens to psoriasis and hidradenitis suppurativa would fit with the preferential homing of fungi in the skin area most involved in those conditions. Fungal antigens also possess autoimmune uveitis-promoting function. As genes associated with SpA (CARD9 and IL23R) strongly regulate the innate immune response against fungi, further studies on fungi contribution to SpA are needed.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb9c2609c7db856b5156dfbf9e51520cTest
https://pubmed.ncbi.nlm.nih.gov/34166798Test