المؤلفون: Huyghebaert, Loreen, Massonnet, Philippe, Grifnée, Elodie, Demeuse, Justine, Peeters, Stéphanie, Dubrowski, Thomas, Schoumacher, Matthieu, Cavalier, Etienne, Le Goff, Caroline

المصدر: CIRM Day 2024, Liège, Belgium [BE], 31/01/2024

مصطلحات موضوعية: Gastrin, LC-MS/MS, Human, Human health sciences, Laboratory medicine & medical technology, Sciences de la santé humaine, Médecine de laboratoire & technologie médicale

الوصول الحر: https://orbi.uliege.be/handle/2268/314615Test

المؤلفون: Huyghebaert, Loreen, Massonnet, Philippe, Grifnée, Elodie, Demeuse, Justine, Dubrowski, Thomas, Schoumacher, Matthieu, Peeters, Stéphanie, Cavalier, Etienne, Le Goff, Caroline

المصدر: RBSLM, Bruxelles, Belgium [BE], 17 novembre 2023

مصطلحات موضوعية: LC-MS/MS, Gastrins, Clinical chemistry, Human health sciences, Laboratory medicine & medical technology, Sciences de la santé humaine, Médecine de laboratoire & technologie médicale

الوصف: editorial reviewed
Gastrin, secreted by G cells, plays a crucial role in digestion and has diverse functions including regulation of the intestinal epithelium and stomach growth.Gastrin peptides are derived from progastrin. Peptides G17 and G34 are the most abundant in the blood. Both of them may be sulfated.Current gastrin measurement relies on the DIAsource RIA kit, however it displays cross-reactivity issues. Therefore, we developed a LC-MS/MS method to quantify both sulfated and non-sulfated G17 and G34 forms.

الوصول الحر: https://orbi.uliege.be/handle/2268/309201Test

المؤلفون: Massonnet, Philippe, Henri, Laure-Anne, Farre Segura, Jordi, Peeters, Stéphanie, Grifnee, Elodie, Demeuse, Justine, Huyghebaert, Loreen, Dubrowski, Thomas, Schoumacher, Matthieu, Le Goff, Caroline, Cavalier, Etienne

المصدر: CIRM day 2023, 01/02/2023

مصطلحات موضوعية: Human health sciences, Laboratory medicine & medical technology, Sciences de la santé humaine, Médecine de laboratoire & technologie médicale

الوصول الحر: https://orbi.uliege.be/handle/2268/300709Test

المؤلفون: Huyghebaert, Loreen, Demeuse, Justine, Grifnee, Elodie, Massonnet, Philippe, Farre Segura, Jordi, Peeters, Stéphanie, Le Goff, Caroline, Cavalier, Etienne

المصدر: International Mass Spectrometry Conference (IMSC) 2022, Maastricht, Netherlands [NL], 29/08/22 - 02/09/22

مصطلحات موضوعية: angiotensin, LC-MS/MS, Human health sciences, Laboratory medicine & medical technology, Sciences de la santé humaine, Médecine de laboratoire & technologie médicale

الوصف: Recent studies showed that angiotensin-converting 2 (ACE2) is used by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as a cellular entry receptor. SARS-CoV-2 causes down regulation of ACE2 leading to renin-angiotensin-aldosterone system (RAAS) major imbalance. This is an essential element of unfavourable evolution in patients with COVID-19. With lower level of ACE2, cleavage of And I and And II is decrease and therefore, And 1-7 and And 1-9 levels are decreased. The development of a quantitative method for these angiotensins is particularly interesting in the context of the prognosis/follow-up of patients with COVID-19. Based on this, this work aims at assessing angiotensins profile variations occurring over time with patients COVID-19 + using a LC-MS/MS method. In this project, plasma samples are prepared with an microelution MAX plate before injection on a Vexera X2 UPLC (Shimadzu Corporation, Kyoto, Japan) coupled to a QT5500 mass spectrometer (Scion, CA, USA) fitted with an IonDriveTMTurbo V ion source and using electro spray ionisation in positive mode. The mobile phase are composed of water (+0,4% formic acid) and of acetonitrile (+0,4% formic acid).

الوصول الحر: https://orbi.uliege.be/handle/2268/295374Test

المؤلفون: Demeuse, Justine, Grifnee, Elodie, Massonnet, Philippe, Huyghebaert, Loreen, Peeters, Stéphanie, Le Goff, Caroline, Cavalier, Etienne

المساهمون: CIRM - Centre Interdisciplinaire de Recherche sur le Médicament - ULiège

المصدر: IMSC 2022, Maastricht, Netherlands [NL], 29/08/22 - 02/09/22

مصطلحات موضوعية: Type I collagen, Mass spectrometry, In-situ digestion, Human health sciences, Laboratory medicine & medical technology, Sciences de la santé humaine, Médecine de laboratoire & technologie médicale

الوصول الحر: https://orbi.uliege.be/handle/2268/295429Test

المؤلفون: Massonnet, Philippe, Peeters, Stéphanie, Grifnee, Elodie, Demeuse, Justine, Huyghebaert, Loreen, Farre Segura, Jordi, Delanaye, Pierre, Le Goff, Caroline, Cavalier, Etienne

المصدر: International Mass Spectrometry Conference (IMSC) 2022, Maastricht, Netherlands [NL], du 29 août au 2 septembre 2022

مصطلحات موضوعية: Human health sciences, Laboratory medicine & medical technology, Sciences de la santé humaine, Médecine de laboratoire & technologie médicale

الوصف: Iohexol is a well-known marker used to evaluate glomerular filtration rate (GFR) which is one indicator of kidney function. This GFR in often calculated using Iohexol intensity decay calculated using LC-MS/MS approaches on human plasma and urines. In these approaches, urines or plasma are taken from patients who were administered Iohexol at different timepoints and Iohexol is quantified at each time using one MRM approach. Once those value obtained, kinetics can be performed and GFR is calculated. However, some discrepancies can occur between urine and plasma results from the same patient and no study clearly explained this. Based on this, this work aims at assessing molecule profile variations occurring over time with patients that took Iohexol using LC coupled with high resolution mass spectrometry.In this project, urine samples are taken at given timepoints from patients who received Iohexol. The samples are first centrifuged, and the supernatant is diluted 100 times with water before injection in a NanoACQUITY UPLC system coupled with a SYNAPT XS instrument operating in positive ion mode. The mobile phases are composed of water (+0.1% formic acid) and of acetonitrile (+0.1% formic acid). Standard samples (commercial Iohexol drug) are also analyzed as quality control.The Iohexol LC-MS/MS method on a triple quadripole instrument has successfully been implemented on the SYNAPT XS – NanoACQUITY UPLC system. The first results on different patient urines show the presence of other peaks that Iohexol in patient samples. Interestingly, those peaks are not present in standards and in urines of patients who did not receive the drug. Investigation of all mass spectra is in progress and these results open the possibility for a large screening over time. Comparison with data obtained using LC-MS/MS is also in progress and the next step is the comparison with data obtained on plasma samples.

الوصول الحر: https://orbi.uliege.be/handle/2268/295373Test

المؤلفون: Demeuse, Justine, Calaprice, Chiara, Huyghebaert, Loreen, Rechchad, Marwa, CAVALIER, Etienne, LE GOFF, Caroline

المصدر: EuroMedLab 2021 Munich, Munich, Germany [DE], 10-14/04/2022

مصطلحات موضوعية: Validation, melatonin, LC-MS/MS, Human health sciences, Laboratory medicine & medical technology, Sciences de la santé humaine, Médecine de laboratoire & technologie médicale

الوصول الحر: https://orbi.uliege.be/handle/2268/291132Test

المؤلفون: Demeuse, Justine, Calaprice, Chiara, LE GOFF, Caroline, CAVALIER, Etienne

المصدر: CIRM DAY 2021, Liège, Belgium [BE], 26/05/2021

مصطلحات موضوعية: LC-MS/MS, melatonin, troubleshooting, Human health sciences, Laboratory medicine & medical technology, Sciences de la santé humaine, Médecine de laboratoire & technologie médicale

الوصول الحر: https://orbi.uliege.be/handle/2268/291062Test

المؤلفون: Demeuse, Justine, Massonnet, Philippe, Grifnee, Elodie, Huyghebaert, Loreen, Schoumacher, Matthieu, Dubrowski, Thomas, Determe, William, Peeters, Stéphanie, Le Goff, Caroline, Cavalier, Etienne

المصدر: CIRM day 2023, Liège, Belgium [BE], 01/02/2023

مصطلحات موضوعية: Human health sciences, Laboratory medicine & medical technology, Sciences de la santé humaine, Médecine de laboratoire & technologie médicale

العلاقة: Introduction : The crosslinked carboxy-terminal telopeptide of type I collagen (B-CTX) is a biomarker used for the follow-up of osteoporotic patients. It is currently uncharacterized, making it impossible to standardise the various immunoassays for B-CTX. Little is known about the structure of the marker, other than the fact that it is a product of type I collagen digestion and consists of three peptides linked by a pyridinoline crosslink.Material and methods : Plasma, serum, and urine pools were processed using two distinct sample preparation protocols. In the first sample preparation, protein precipitation was used to prepare and concentrate the pools. Using preparative liquid chromatography, samples were then separated, and fractions were collected every 15 seconds. Following evaporation, fractions were reconstituted with 30 uL of H2O, 0,4% FA. The other sample preparation consisted of affinity chromatography with two antibodies directed against a well-known portion of B-CTX. After samples were purified, they were evaporated and reconstituted with 30uL of injection solvent. Both protocols' samples were then injected into a high resolution mass spectrometer (Synapt XS, Waters). The mass spectra we obtained were then analyzed by PLGS and Peaks Studio for type I collagen peptides identification.Results : We were able to identify ten uncrosslinked peptides of type I collagen containing the crosslink site so far but additional database searches are currently underway to identify crosslinked peptides. These findings would lead to a full characterization of B-CTX. Conclusion : The successful development of our workflow led to the identification of several candidates. Confirmation of these candidates needs further investigation.

الوصول الحر: https://orbi.uliege.be/handle/2268/299817Test

المؤلفون: Massonnet, Philippe, Huyghebaert, Loreen, Grifnée, Elodie, Demeuse, Justine, Determe, William, Schoumacher, Matthieu, Dubrowski, Thomas, Cavalier, Etienne, Le Goff, Caroline

المصدر: 2023 Annual RBSLM Meeting, Bruxelles, Belgium [BE], 17/11/2023

مصطلحات موضوعية: Human health sciences, Laboratory medicine & medical technology, Sciences de la santé humaine, Médecine de laboratoire & technologie médicale

الوصف: Parathyroid hormone-related protein (PTHrp) is a protein that has a high sequence similarity with the parathyroid hormone (PTH). In humans, 3 main species are reported : 1-139 ; 1-173 and 1-141. Because of their sequence similarities, PTH and PTHrp act on the same biological receptor. By opposition to PTH, that has one endocrine activity, PTHrp has one paracrine activity. Produced in high amount by cancer cells, PTHrp is known to be at the origin of the tumor hypercalcemia syndrome. For now, PTHrp is mainly quantified using radio-immuno assay (RIA). However, these assays shows a lot of cross-reactivities, making difficult a good interpretation of the results. In order to overcome these problems, the goal of this study is to develop one LC-MS/MS method for the quantitation of PTHrp in human plasma.

الوصول الحر: https://orbi.uliege.be/handle/2268/312729Test