المؤلفون: Reetu Kundu, Amber Parwaiz, Parikshaa Gupta, Manish Rohilla, Nalini Gupta, Radhika Srinivasan, Pranab Dey

المصدر: Cytopathology. 33:678-687

مصطلحات موضوعية: Male, Histology, Cytodiagnosis, Biomarkers, Tumor, Ascitic Fluid, Humans, Female, General Medicine, Adenocarcinoma, Immunohistochemistry, Pleural Effusion, Malignant, Pathology and Forensic Medicine

الوصف: Malignant effusions are commonly encountered in day-to-day cytology practice. Determining the primary site of malignancy in carcinomatous effusions is a Herculean task. Cytology coupled with immunocytochemistry (ICC) is often found to be helpful in this context.This study was conducted to evaluate the diagnostic utility of ICC on sections from cell blocks (CBs) in the detection of the primary site of origin in cases of metastatic carcinomatous effusions. To determine the origin of the primary tumour, TTF1 (lung), PAX-8 (ovary), CDX2 (colorectal), GATA3 (breast), and CK19 (pancreaticobiliary) were employed, depending on the clinical and radiological findings, and serum tumour markers.A total of 13,459 serous effusion samples were received for cytological evaluation from January 2017 to December 2021, of which 2708 (20.1%) were carcinomatous effusions. Out of these, 1044 (38.5%), 1611 (59.5%), and 53 (2.0%) were from pleural, peritoneal and pericardial cavities, respectively. Of these, the majority were adenocarcinoma. ICC was performed in 309 (11.4%) cases. The ovary was the most common primary site in 179 cases (57.9%), followed by the lung (75, 24.3%), pancreaticobiliary system (12, 3.9%), colon/rectum (8, 2.6%), breast (6, 1.9%), prostate (2, 0.6%) and kidney (1, 0.3). The lung was the most common primary site in pleural (67/113, 59.3%) and pericardial (6/8, 75%) effusions. The ovary (168/188, 89.4%) was the most common primary site for carcinomatous effusions in the peritoneal cavity. However, in 17 (5.5%) cases, the exact primary site could not be established.Judicious and methodical use of ICC on CBs helps to identify the primary site of the tumour in most carcinomatous effusions. This is of immense help to the treating clinician in directing appropriate therapy.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f19ccf5f3f7325e97b1d643917662eeTest
https://doi.org/10.1111/cyt.13161Test

المؤلفون: Tarunpreet Saini, Reetu Kundu, Pulkit Rastogi, Manish Rohilla, Parikshaa Gupta, Nalini Gupta, Radhika Srinivasan, Pranab Dey

المصدر: Cytopathology. 33:493-498

مصطلحات موضوعية: Male, Ovarian Neoplasms, Histology, Testicular Neoplasms, Teratoma, Humans, Female, Neoplasms, Second Primary, Dysgerminoma, General Medicine, Neoplasms, Germ Cell and Embryonal, Pathology and Forensic Medicine

الوصف: Germ cell tumours infrequently metastasise to body cavities, where early detection on fluid samples is possible and can spearhead early treatment and survival.A total of seven cases of metastatic germ cell tumours were retrieved out of 7500 effusion samples received for cytopathological examination from 2015 to 2021. Detailed cytological features of metastatic germ cell tumours in effusion samples were studied, along with a correlation between clinical, radiological, and histopathological features.A total of seven cases of metastatic germ cell tumours were analysed in effusion samples which included dysgerminoma (2), immature teratoma (2), yolk sac tumour (1), embryonal carcinoma (1), and mixed germ cell tumour (1). The smears showed predominantly discrete or loose clusters of cells. The cells with round nuclei and prominent nucleoli were helpful in detecting dysgerminoma and yolk sac tumours. Immature teratoma showed tiny groups of small cells and mature squamous cells. Serum tumour markers were raised in the majority of cases.Metastatic germ cell tumours in effusion are uncommon, but detailed clinical history, including serum markers and characteristic cytological features, are helpful in their diagnosis.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b81893b73982336428a4fc7a1544427Test
https://doi.org/10.1111/cyt.13122Test

المؤلفون: Malvika Shastri, Reetu Kundu, Manish Rohilla, Parikshaa Gupta, Nalini Gupta, Radhika Srinivasan, Uma Nahar Saikia, Pranab Dey

المصدر: Diagnostic cytopathologyREFERENCES. 50(8)

مصطلحات موضوعية: Male, Histology, Thyroid Cancer, Papillary, Biopsy, Fine-Needle, Carcinoma, Humans, Female, General Medicine, Thyroid Neoplasms, Carcinoma, Papillary, Pathology and Forensic Medicine

الوصف: To describe the cytomorphological features at distant metastatic sites apart from lymph nodes in papillary thyroid carcinoma (PTC).A total of eight cytology cases from seven patients reported as metastatic papillary carcinoma of thyroid were reviewed including available clinical and radiological details. The details clinical and cytological features were studied.There were a total of five males and two female patients. Two of these patients presented with lung nodules, two patients had bony lesion and lesion in kidney respectively and one patient had both bony and soft tissue lesions. Pleural fluid and bronchoalveolar lavage were received from remaining two patients. Cytology smears revealed papillary clusters and sheets of tumor cells, having round to oval nuclei with pale powdery chromatin, inconspicuous nucleoli and scant to moderate amount of cytoplasm. Nuclear features such as longitudinal nuclear grooves, intranuclear cytoplasmic inclusions were seen in all but one case. Nuclear enlargement and overlapping were variably present.The diagnosis of metastatic PTC at uncommon locations is difficult. Since many of these locations are amenable to fine needle aspiration or exfoliative cytology evaluation, clinical awareness, and identification of classic cytomorphologic features can offer quick and accurate diagnoses. In cases with occult or unknown PTC presenting with metastases, cytomorphology may help in the prompt suggestion of detecting primary tumor in the thyroid.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fa839dff7b42fa8cbdbfafa77489c954Test
https://pubmed.ncbi.nlm.nih.gov/35445581Test

المؤلفون: Nalini Gupta, Parikshaa Gupta, Manish Rohilla, Radhika Srinivasan, Arvind Rajwanshi, Pranab Dey, Aleena Jain, Shruti Gupta

المصدر: Diagnostic Cytopathology. 49:226-231

مصطلحات موضوعية: Male, medicine.medical_specialty, Histology, Tuberculosis, Cytodiagnosis, Biopsy, Fine-Needle, Population, 030209 endocrinology & metabolism, Pathology and Forensic Medicine, 03 medical and health sciences, 0302 clinical medicine, Lymphadenitis, Cytology, Humans, Medicine, Lymphocytes, education, Histiocyte, Retrospective Studies, education.field_of_study, Granuloma, business.industry, Infant, General Medicine, Adenitis, medicine.disease, Dermatology, Giant cell, 030220 oncology & carcinogenesis, BCG Vaccine, Primary immunodeficiency, Female, Lymph Nodes, business, Complication

الوصف: Background Bacille Calmette-Guerin (BCG) adenitis is a common complication of post-BCG vaccination. Awareness of this lesser known entity in fine-needle aspiration (FNA) is important to prevent misdiagnosis. Aim This study described the clinical presentation, cytomorphological features and disease pattern in patients with BCG adenitis. Materials and methods We analyzed a series of 15 patients with BCG adenitis seen from July 2017 to July 2019. The detailed clinical and cytomorphology of these cases were studied. Results Age of presentation ranged from 3 to 12 months. Male to female ratio was 2.75:1. A total of four children were diagnosed with primary immunodeficiency. Three children had acyanotic heart disease. Most of the children had left axillary lymph node enlargement. Cytomorphology showed the presence of polymorphs (66.7%), histiocytic aggregates (80%) and reactive lymphoid population (60%). Epithelioid granulomas (26.7%) and giant cells (6.7%) were present. The necrotic background was present in all but one case. Ziehl-Neelsen stain for acid-fast bacilli was positive in all but two cases. Conclusion A high index of clinical suspicion for BCG lymphadenitis should be kept in mind for patients who are recently vaccinated. FNA cytology, combined with clinical correlation, is useful for diagnosis and management.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::103c144cc2ba7ad7c05f50b0e5b0cd81Test
https://doi.org/10.1002/dc.24613Test

المؤلفون: Manish Rohilla, Gargi Kapatia, Parikshaa Gupta, Radhika Srinivasan, Pranab Dey, Arvind Rajwanshi, Ojas Gupta, Uma Nahar Saikia, Nalini Gupta

المصدر: Cytopathology. 31:136-143

مصطلحات موضوعية: Adult, Male, Pathology, medicine.medical_specialty, Histology, Adolescent, Gastrointestinal Stromal Tumors, Cytodiagnosis, Biopsy, Fine-Needle, Immunocytochemistry, Rectum, 030209 endocrinology & metabolism, Pathology and Forensic Medicine, Metastasis, Diagnosis, Differential, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Biomarkers, Tumor, medicine, Humans, Neoplasm Metastasis, neoplasms, Aged, Aged, 80 and over, GiST, business.industry, Stomach, Mediastinum, Neoplasms, Second Primary, General Medicine, Middle Aged, medicine.disease, digestive system diseases, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Duodenum, Female, business, Epithelioid cell

الوصف: Aim To explore the cytological spectrum of the gastrointestinal stromal tumour (GIST) including its metastatic sites. Material and methods A total of 42 patients (45 sites) diagnosed with GIST or its metastases on fine needle aspiration cytology were studied over a period of 5 years. May-Grunwald Giemsa- and haematoxylin and eosin-stained smears were reviewed and analysed for the cytomorphological spectrum of GIST. Results Primary GIST alone was seen in 24 cases, E-GIST in eight cases and metastasis in 11 cases (one patient showing metastasis at two distinct sites), whereas concurrent primary and metastatic lesions were noted in two cases. Amongst primary sites, the most commonly affected location was stomach (n = 22), followed by ileum (n = 2), duodenum (n = 1) and rectum (n = 1). Extra-GIST was seen in retroperitoneum and pelvis (n = 3 each), omentum and mediastinum (n = 1 each). Fine needle aspiration cytology was done from 11 metastatic sites of GIST which included liver, gall bladder fossa, chest wall, and thigh. The classic spindle cell arrangement was the predominant cytological pattern. About 8.8% cases showed predominant epithelioid cell morphology and 15.5% cases had a mixed cytomorphology comprising of both spindle cell and epithelioid cell patterns. Nuclear pseudoinclusions, perinuclear vacuoles and multinucleation were seen in four cases. Immunocytochemistry on cell-block sections for confirmation was performed in 18 cases and all these cases showed strong c-KIT positivity. Conclusion In this largest case series of cytomorphological diagnosis of GIST, we describe the cytomorphology and immunocytochemistry of primary and metastatic GIST. GISTs with predominant epithelioid cell morphology may pose a diagnostic dilemma therefore in all suspected cases of GIST, immunocytochemistry for c-KIT and/or DOG1 should be employed on cell-block preparations to confirm the diagnosis of GIST.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74c0311eeef623b2cd8a063ebfa16d53Test
https://doi.org/10.1111/cyt.12785Test

المؤلفون: Radhika Srinivasan, Arvind Rajwanshi, Ashmita Saha, Gargi Kapatia, Parikshaa Gupta, Pranab Dey, Nalini Gupta, Manish Rohilla

المصدر: Acta Cytologica. 64:532-538

مصطلحات موضوعية: Adult, Male, Pathology, medicine.medical_specialty, Histology, Adolescent, Cytodiagnosis, Biopsy, Fine-Needle, Giant Cells, Histoplasmosis, Pathology and Forensic Medicine, Diagnosis, Differential, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Histoplasma, Cytology, medicine, Humans, Child, Lymph node, Histiocyte, Aged, Aged, 80 and over, Granuloma, Lung, biology, medicine.diagnostic_test, business.industry, General Medicine, Middle Aged, 030224 pathology, biology.organism_classification, medicine.disease, medicine.anatomical_structure, Fine-needle aspiration, Giant cell, 030220 oncology & carcinogenesis, Female, Lymph Nodes, business

الوصف: Objective: The aim of the study was to study the clinical and morphological spectrum of histoplasmosis diagnosed by fine needle aspiration cytology (FNAC). Study Design: A total of 17 patients diagnosed with histoplasmosis on FNAC were studied over a period of 5 years. The cytology smears were studied and analysed for the cytomorphological spectrum of histoplasmosis. Results: Among the 17 patients studied, the mean age was 51.3 years (range 6–84 years). Male-to-female ratio was 3.25:1 with 4 females and 13 males. The frequent sites of involvement were the lung (5), lymph node (4), adrenal gland (4), and skin (4). The most common cytological patterns were histiocytic collection, followed by granulomas and multinucleated giant cells. Necrosis was noted in only 4 cases. Conclusion: Fine needle aspiration is a highly accurate, rapid, and cheap technique for the diagnosis of histoplasmosis due to its distinct morphological features.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e25219d2c3605969b1e0e607caac9c15Test
https://doi.org/10.1159/000509151Test

المؤلفون: Nalini Gupta, Nandita Kakkar, Richa Jain, Radhika Srinivasan, Abin Koshy, Arvind Rajwanshi, Pranab Dey, Prateek Bhatia, Deepak Bansal, Amita Trehan

المصدر: Cytopathology. 30:634-643

مصطلحات موضوعية: Male, Pathology, medicine.medical_specialty, Histology, Cytodiagnosis, Biopsy, Fine-Needle, Immunocytochemistry, 030209 endocrinology & metabolism, Pathology and Forensic Medicine, Diagnosis, Differential, Neuroblastoma, 03 medical and health sciences, 0302 clinical medicine, Cytology, Humans, Medicine, Ganglioneuroma, Child, Ganglioneuroblastoma, medicine.diagnostic_test, business.industry, Infant, Newborn, Infant, General Medicine, Prognosis, medicine.disease, Peripheral, Fine-needle aspiration, Cytopathology, Child, Preschool, 030220 oncology & carcinogenesis, Female, business

الوصف: OBJECTIVE The aim of this analysis was to describe the cytopathology spectrum of peripheral neuroblastic tumours (NTs) including neuroblastoma (NB), ganglioneuroblastoma (GNB) and ganglioneuroma (GN). Feasibility of applying the International Neuroblastoma Pathology Classification (INPC) to further subtype NTs in cytology was evaluated. METHODS All peripheral NTs reported on fine needle aspiration during 2011-2015 were retrieved and detailed cytomorphological evaluation was performed. Based on INPC criteria, NBs were further categorised as undifferentiated, poorly differentiated and differentiating subtypes. Mitotic-karyorrhectic index was evaluated. Immunocytochemistry on cell blocks was reviewed wherever available. MYCN amplification by fluorescence in situ hybridisation was performed in 11 cases on smears. RESULTS A total of 90 cases including 83 NBs, six GNB and one GN were evaluated. The age range was 12 days-12 years, with 55 males and 45 females. Both the primary and metastatic locations were aspirated. Applying the INPC criteria, there were 61 poorly differentiated, 14 undifferentiated, eight differentiating NB and six GNB. Immunocytochemistry on cell blocks showed positivity for at least two neuronal markers in NB. Mitotic-karyorrhectic index was high in 63, low in 22 and intermediate in two cases, respectively. MYCN amplification by fluorescence in situ hybridisation was feasible on smears and was amplified in 6 out of 11 cases tested. CONCLUSION Peripheral NT types including NB, GNB and GN have distinctive cytomorphology. NBs can be further subtyped as undifferentiated, poorly differentiated and differentiating subtypes as per INPC criteria.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17502b36544ef5b903bf9e1188a50048Test
https://doi.org/10.1111/cyt.12747Test

المؤلفون: Nalini Gupta, Radhika Srinivasan, Arvind Rajwanshi, Nandita Kakkar, Pranab Dey, Ram Samujh, Parimal Agrawal

المصدر: Cytopathology. 30:301-308

مصطلحات موضوعية: Male, Pathology, medicine.medical_specialty, Histology, Cytodiagnosis, Biopsy, Fine-Needle, Soft Tissue Neoplasms, 030209 endocrinology & metabolism, Pathology and Forensic Medicine, Diagnosis, Differential, 03 medical and health sciences, 0302 clinical medicine, Fine needle aspiration cytology, Cytology, medicine, Humans, Child, Radiation treatment planning, Retrospective Studies, medicine.diagnostic_test, business.industry, Infant, Myxoma, Soft tissue, General Medicine, medicine.disease, Immunohistochemistry, Fine-needle aspiration, Child, Preschool, 030220 oncology & carcinogenesis, Female, Lipoblastoma, Histopathology, business

الوصف: Introduction The type of soft tissue lesions seen in children differs from that seen in adults. The role of fine needle aspiration (FNA) cytology in their diagnosis is not well documented. Aim To study the cytopathological spectrum of paediatric soft tissue tumours to highlight uncommon benign and malignant lesions and the challenges in their diagnosis. Methods A 3-year retrospective audit of all paediatric soft tissue FNA cytology cases from 2015 to 2017 was performed. Smears were reviewed along with cell block immunocytochemistry and follow-up histopathology of resected specimens wherever available. Results A total of 127 cases were reviewed, which included 72 benign and 55 malignant soft tissue tumours. Uncommon lesions described herein are myxoid fibrohistiocytic tumour, myxoma, lipoblastoma, Bednar tumour, malignant extra-renal rhabdoid tumour and desmoplastic small round cell tumour. Histopathology confirmation was available in 25 cases, out of which 16 cases were completely concordant. In eight cases, all benign diagnoses, histopathology provided more accurate subtyping than FNA. These included cases of lipoblastoma, myxoma and spindle cell haemangioma. Conclusion FNA cytology of paediatric soft tissue tumours is accurate in classifying lesions as benign or malignant which helps in treatment planning. Immunocytochemistry performed on cell blocks is useful for subtyping malignant lesions.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ea8f8bed7bb99caf2300484d23e80ffeTest
https://doi.org/10.1111/cyt.12685Test

المؤلفون: Karine Hovanes, Kurosh Rahimi, Louise Lapensée, Ngoc Minh Phuong Nguyen, Ramesh Reddy, Philippe Sauthier, Trilochan Sahoo, Feride Iffet Sahin, Matthew Osmond, Rima Slim, Zhao-Jia Ge, Magali Breguet, Jacek Majewski, Teruko Taketo, Asangla Ao, Somayyeh Fahiminiya, Radhika Srinivasan, Rashmi Bagga, Ignatia B. Van den Veyver, Sangeetha Mahadevan

المصدر: The American Journal of Human Genetics. 103:740-751

مصطلحات موضوعية: Male, 0301 basic medicine, Zygote, female infertility, Biology, male infertility, recurrent hydatidiform moles, Chromosomes, Article, Male infertility, recurrent miscarriages, Andrology, Mice, 03 medical and health sciences, Human fertilization, Meiosis, Pregnancy, Genetics, medicine, TOP6BL, Animals, Humans, REC114, Alleles, Genetics (clinical), MEI1, Mammals, Female infertility, Embryo, Hydatidiform Mole, medicine.disease, Sperm, Mice, Inbred C57BL, 030104 developmental biology, Mutation, Androgens, Oocytes, Female, Ploidy

الوصف: Androgenetic complete hydatidiform moles are human pregnancies with no embryos and affect 1 in every 1,400 pregnancies. They have mostly androgenetic monospermic genomes with all the chromosomes originating from a haploid sperm and no maternal chromosomes. Androgenetic complete hydatidiform moles were described in 1977, but how they occur has remained an open question. We identified bi-allelic deleterious mutations in MEI1, TOP6BL/C11orf80, and REC114, with roles in meiotic double-strand breaks formation in women with recurrent androgenetic complete hydatidiform moles. We investigated the occurrence of androgenesis in Mei1-deficient female mice and discovered that 8% of their oocytes lose all their chromosomes by extruding them with the spindles into the first polar body. We demonstrate that Mei1−/− oocytes are capable of fertilization and 5% produce androgenetic zygotes. Thus, we uncover a meiotic abnormality in mammals and a mechanism for the genesis of androgenetic zygotes that is the extrusion of all maternal chromosomes and their spindles into the first polar body. © 2018 American Society of Human Genetics

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::40b192fdb19d383fe5aadd54ae1aa67cTest
https://doi.org/10.1016/j.ajhg.2018.10.007Test

المؤلفون: Nalini Gupta, Pranab Dey, Upasana Gautam, Manish Rohilla, Neelam Varma, Parikshaa Gupta, Radhika Srinivasan, Arvind Rajwanshi, Tushar Pandey

المصدر: Diagnostic cytopathologyREFERENCES. 49(5)

مصطلحات موضوعية: Adult, Male, Pathology, medicine.medical_specialty, Histology, Adolescent, Chronic lymphocytic leukemia, Cytodiagnosis, Follicular lymphoma, 030209 endocrinology & metabolism, Pericardial Effusion, Pathology and Forensic Medicine, 03 medical and health sciences, Young Adult, 0302 clinical medicine, immune system diseases, hemic and lymphatic diseases, medicine, Ascitic Fluid, Humans, Hairy cell leukemia, Child, Aged, Retrospective Studies, Aged, 80 and over, business.industry, Lymphoblastic lymphoma, Infant, General Medicine, Middle Aged, medicine.disease, Flow Cytometry, Immunohistochemistry, Lymphoproliferative Disorders, Lymphoma, Pleural Effusion, Malignant, Leukemia, Serous fluid, 030220 oncology & carcinogenesis, Child, Preschool, Female, business, Plasmablastic lymphoma

الوصف: Introduction Involvement of body fluids by lymphoreticular malignancies (LRM) is rare and often associated with poor prognosis and decreased overall survival. The present study was conducted to analyze the characteristic cytomorphologic, flow cytometric and immunocytochemical features of LRMs in serous effusions. Materials and methods This was a three-year retrospective study. A total of 218 effusion samples, reported as involved by lymphoreticular malignancies, on cytology, were reviewed. All the cases wherein the cytological diagnosis was confirmed by flow cytometric (FCM) and/or immunocytochemical (ICC) studies were retrieved and studied in detail. FCM and/or ICC were performed in a total of 51/218(23.4%) samples, including 30 pleural (58.8%), 18 peritoneal (35.3%), and 3 pericardial fluid (5.9%) samples. Results The cytomorphologic diagnoses included infiltration by non-Hodgkin lymphoma (NHL;n = 27), infiltration by LRM (n = 19), infiltration by chronic lymphocytic leukemia (CLL;n = 2), Hodgkin's lymphoma (HL;n = 1) and suggestive of infiltration by LRM (n = 2). FCM and/or ICC confirmed the diagnoses as infiltration by T-cell lymphoblastic lymphoma in 18; mature B-cell NHL in 10; Burkitt lymphoma in 7; diffuse large B-cell lymphoma in 4; follicular lymphoma, T- cell NHL and CLL in 2 samples each and hairy cell leukemia, plasmablastic lymphoma and HL in 1 sample each. 94.1% concordance was noted between the initial and final cytologic diagnosis. Conclusions Involvement of body fluids and effusions by LRMs, though rare, carries an immense prognostic significance and hence the prompt detection is crucial. Detection of these malignancies by cytologic examination of effusions is challenging yet potentially useful and the least invasive method available to establish an early diagnosis.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::498593f8926f3ae02454335896051343Test
https://pubmed.ncbi.nlm.nih.gov/33629825Test