دورية أكاديمية
Identification of molds with MALDI-TOF mass spectrometry: performance of the newly developed MSI-2 application in comparison with the Bruker filamentous fungi database and MSI-1
| العنوان: | Identification of molds with MALDI-TOF mass spectrometry: performance of the newly developed MSI-2 application in comparison with the Bruker filamentous fungi database and MSI-1 |
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| المؤلفون: | Normand, Anne-Cécile, Blaize, Marion, Imbert, Sébastien, Packeu, Ann, Becker, Pierre, Fekkar, Arnaud, Stubbe, Dirk, Piarroux, Renaud |
| المساهمون: | CHU Pitié-Salpêtrière AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Sciensano Bruxelles, Réseau International des Instituts Pasteur (RIIP), Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU) |
| المصدر: | ISSN: 0095-1137 ; Journal of Clinical Microbiology ; https://hal.sorbonne-universite.fr/hal-03349849Test ; Journal of Clinical Microbiology, 2021, 59 (10), ⟨10.1128/JCM.01299-21⟩. |
| بيانات النشر: | HAL CCSD American Society for Microbiology |
| سنة النشر: | 2021 |
| المجموعة: | Réseau International des Instituts Pasteur, Paris: HAL-RIIP |
| مصطلحات موضوعية: | MALDI-TOF mass spectrometry, mold, online identification, MSI, database, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
| الوصف: | International audience ; Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) represents a promising tool for the rapid and efficient identification of molds, but improvements are still necessary to achieve satisfactory results when identifying cryptic species. Here, we aimed to validate a new web application, MSI-2, which replaces MSI-1, an application that was built and deployed online in 2017. For the evaluation, we gathered 633 challenging isolates obtained from daily hospital practice that were first identified with DNA-based methods, and we submitted their corresponding mass spectra to three identification programs (Bruker, MSI-1 and MSI-2). The MSI-2 application had a better identification performance at the species level than MSI-1 and Bruker, reaching 83.25% correct identifications compared with 63.19% (MSI-1), 38.07% (Bruker with 1.7 threshold) and 21.8% (Bruker with 2.0 threshold). The MSI-2 application performed especially well for Aspergillus and Fusarium species, including for many cryptic species, reaching 90% correct identifications for Aspergillus species and 78% for Fusarium species compared to 69% and 43% with MSI-1. Such improvement may have a positive impact on patient management by facilitating the identification of cryptic species potentially associated with a specific antifungal resistance profile. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-03349849; https://hal.sorbonne-universite.fr/hal-03349849Test; https://hal.sorbonne-universite.fr/hal-03349849/documentTest; https://hal.sorbonne-universite.fr/hal-03349849/file/Normand%20et%20al.%20-%20Identification%20of%20molds%20with%20MALDI-TOF%20mass%20spectr.pdfTest |
| DOI: | 10.1128/JCM.01299-21 |
| الإتاحة: | https://doi.org/10.1128/JCM.01299-21Test https://hal.sorbonne-universite.fr/hal-03349849Test https://hal.sorbonne-universite.fr/hal-03349849/documentTest https://hal.sorbonne-universite.fr/hal-03349849/file/Normand%20et%20al.%20-%20Identification%20of%20molds%20with%20MALDI-TOF%20mass%20spectr.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.918CED71 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/JCM.01299-21 |
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