التفاصيل البيبلوغرافية
| العنوان: |
Comparative transcriptomics reveals common and strain-specific responses of human macrophages to infection with Mycobacterium tuberculosis and Mycobacterium bovis BCG. |
| المؤلفون: |
Li, Pei1,2 (AUTHOR), Li, Yang1 (AUTHOR), Wang, Cun Chuan1 (AUTHOR), Xia, Li Gang1 (AUTHOR) ligangxiaszph@hotmail.com |
| المصدر: |
Microbial Pathogenesis. Apr2024, Vol. 189, pN.PAG-N.PAG. 1p. |
| مصطلحات موضوعية: |
*MYCOBACTERIA, *MYCOBACTERIUM bovis, *MYCOPLASMA bovis, *MYCOBACTERIAL diseases, *TRANSCRIPTOMES, *BURULI ulcer, *MACROPHAGES, *VIRAL tropism |
| مصطلحات جغرافية: |
TOKYO (Japan) |
| مستخلص: |
Mycobacterium tuberculosis (MTB) and Mycobacterium bovis (M. bovis) are closely related pathogenic mycobacteria known to cause chronic pulmonary infections in both humans and animals. Despite sharing nearly identical genomes and virulence factors, these two bacteria display variations in host tropism, epidemiology, and clinical presentations. M. bovis Bacillus Calmette–Guérin (BCG) is an attenuated strain of M. bovis commonly utilized as a vaccine for tuberculosis (TB). Nevertheless, the molecular underpinnings of these distinctions and the intricacies of host-pathogen interactions remain areas of ongoing research. In this study, a comparative transcriptomic analysis was conducted on human leukemia macrophages (THP-1) infected with either MTB H37Rv or M. bovis BCG (Tokyo strain) to elucidate common and strain-specific responses at the transcriptional level. RNA sequencing was utilized to characterize the transcriptomes of human primary macrophages infected with MTB or BCG at 6 and 24 h post-infection. The findings indicate that both MTB and BCG induce substantial and dynamic alterations in the transcriptomes of THP-1, with a notable overlap in the quantity and extent of differentially expressed genes (DEGs). Moreover, gene ontology (GO) enrichment analysis unveiled shared pathways related to immune response, cytokine signaling, and apoptosis. The immune response of macrophages to bacterial infections at 6 h exhibited significantly greater intensity compared to that at 24 h. Furthermore, distinct gene sets displaying notable variances between MTB and BCG infections were identified. The profound impact of MTB infection on macrophage gene expression, particularly within the initial 6 h, was evident. Additionally, downregulation of pathways such as Focal adhesion, Rap1 signaling pathway, and Regulation of actin cytoskeleton was observed. The pathways associated with inflammation reactions and cell apoptosis exhibited significant differences, with BCG triggering macrophage apoptosis and MTB enhancing the survival of intracellular bacteria. Our findings reveal that MTB and BCG provoke similar yet distinct transcriptional responses in human macrophages, indicating variations in their pathogenesis and ability to adapt to host environments. These results offer novel insights into the molecular mechanisms governing host-pathogen interactions and may contribute to a deeper understanding of TB pathogenesis. • Both MTB and BCG trigger robust and dynamic changes in the transcriptomes of THP-1. • Dramatic impact of MTB infection on macrophage gene expression within 6 hpi. • Classical signaling pathways are crucial in mediating immunity during MTB infection. • BCG induces apoptosis in macrophages at early stage. • MTB shows stronger anti-phagocytosis and anti-macrophage cytotoxicity abilities. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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