Ethanol Extract of Lilium Bulbs Plays an Anti-Inflammatory Role by Targeting the IKKα/β-Mediated NF-κB Pathway in Macrophages
| العنوان: | Ethanol Extract of Lilium Bulbs Plays an Anti-Inflammatory Role by Targeting the IKKα/β-Mediated NF-κB Pathway in Macrophages |
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| المؤلفون: | Hyun-Sik Hwang, Jae Youl Cho, Young-Su Yi, Yo Han Hong, Jongsung Lee, Jong-Hoon Kim, Kang Jun Choi, Mohammad Amjad Hossain, Sang Yun Han, Seong-Gu Jeong, Ho Sik Rho |
| المصدر: | The American Journal of Chinese Medicine. 46:1281-1296 |
| بيانات النشر: | World Scientific Pub Co Pte Lt, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Lipopolysaccharide, biology, Biological activity, NF-κB, General Medicine, IκB kinase, Molecular biology, Nitric oxide, Nitric oxide synthase, 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, Complementary and alternative medicine, chemistry, biology.protein, Tumor necrosis factor alpha, Luteolin |
| الوصف: | Lilium bulbs have long been used as Chinese traditional medicines to alleviate the symptoms of various human inflammatory diseases. However, mechanisms of Lilium bulb-mediated anti-inflammatory activity and the bioactive components in Lilium bulbs remain unknown. In the present study, the anti-inflammatory activity of Lilium bulbs and the underlying mechanism of action were investigated in macrophages using Lilium bulb ethanol extracts (Lb-EE). In a dose-dependent manner, Lb-EE inhibited nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 cells and bone marrow-derived macrophages (BMDMs) without causing significant cytotoxicity. Lb-EE also down-regulated mRNA expression of inflammatory genes in LPS-stimulated RAW264.7 cells, which included inducuble nitric oxide synthase (iNOS), cyclooxygenase-2 (COX2), and tumor necrosis factor-[Formula: see text] (TNF-[Formula: see text]). Furthermore, Lb-EE markedly restored LPS-induced morphological changes in RAW264.7 cells to a normal morphology. HPLC analysis identified quercetin, luteolin, and kaempferol as bioactive components contained in Lb-EE. Mechanistic studies in LPS-stimulated RAW264.7 cells revealed that Lb-EE suppressed MyD88- and TRIF-induced NF-[Formula: see text]B transcriptional activation and the nuclear translocation of NF-[Formula: see text]B transcription factors. Moreover, Lb-EE inhibited IKK[Formula: see text]/[Formula: see text]-induced activation of the NF-[Formula: see text]B signaling pathway and IKK inhibition significantly reduced NO production in LPS-stimulated RAW264.7 cells. Taken together, these results suggest that Lb-EE plays an anti-inflammatory role by targeting IKK[Formula: see text]/[Formula: see text]-mediated activation of the NF-[Formula: see text]B signaling pathway during macrophage-mediated inflammatory responses. |
| تدمد: | 1793-6853 0192-415X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::ea375b3c806057cb6d01bf1f59bd468eTest https://doi.org/10.1142/s0192415x18500672Test |
| رقم الانضمام: | edsair.doi...........ea375b3c806057cb6d01bf1f59bd468e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17936853 0192415X |
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