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1دورية أكاديمية
مصطلحات موضوعية: Male Reproductive Health, Reproductive Medicine, Medicine, Health Sciences, Fertility Preservation in Cancer Patients, Public Health, Environmental and Occupational Health, Global Trends in Infertility and Reproductive Technologies, Ovarian Tissue Cryopreservation, Semen Analysis, Sertoli cell, Spermatogenesis, Biology, Andrology, SOX9, Germ cell, Cryopreservation, Testicle, Internal medicine, Testosterone patch, Endocrinology, Gonocyte, Leydig cell, Organ culture, Hormone, Cell biology, In vitro, Embryo, Gene expression, Luteinizing hormone, Biochemistry
الوصف: Background Cryopreservation of immature testicular tissue (ITT) is currently the only option to preserve fertility of prepubertal patients. Autologous transplantation of ITT may not be safe or appropriate for all patients. Therefore, methods to mature ITT ex vivo are needed. Objectives Aim to investigate the feasibility of inducing in vitro spermatogenesis from ITT cryopreserved for pediatric patients prior to initiation of gonadotoxic therapy. Materials and methods Cryopreserved-thawed ITT from prepubertal and peripubertal patients were cultured for 7, 16, and 32 days in medium with no hormones or supplemented with 5 IU/L FSH, 1 IU/L hCG, or 5IU/L FSH+1 IU/L hCG. Samples were evaluated histologically to assess tissue integrity, and immunofluorescence staining was performed to identify VASA (DDX4)+ germ cells, UCHL1+ spermatogonia, SYCP3+ spermatocytes, CREM+ spermatids, SOX9+ Sertoli cells. Proliferation (KI67) and apoptosis (CASPASE3) of germ cells and Sertoli cells were also analyzed. Sertoli and Leydig ... : الحفاظ على أنسجة الخصية غير الناضجة بالتبريد هو الخيار الوحيد حاليًا للحفاظ على خصوبة مرضى ما قبل البلوغ. قد لا يكون الزرع الذاتي لـ ITT آمنًا أو مناسبًا لجميع المرضى. لذلك، هناك حاجة إلى طرق لتنضج ITT خارج الجسم الحي. تهدف الأهداف إلى التحقيق في جدوى تحفيز تكوين الحيوانات المنوية في المختبر من العلاج بالتبريد لدى مرضى الأطفال قبل بدء العلاج السام للغدد التناسلية. تم استزراع المواد والطرق التي تم إذابة ITT المحفوظة بالتبريد من مرضى ما قبل البلوغ وحول البلوغ لمدة 7 و 16 و 32 يومًا في المتوسط بدون هرمونات أو مكملة بـ 5 وحدة دولية/لتر FSH أو 1 وحدة دولية/لتر من قوات حرس السواحل الهايتية أو 5 وحدة دولية/لتر من قوات حرس السواحل الهايتية + 1 وحدة دولية/لتر من قوات حرس السواحل الهايتية. تم تقييم العينات من الناحية النسيجية لتقييم سلامة الأنسجة، وتم إجراء تلوين التألق المناعي لتحديد الأوعية (DDX4)+ الخلايا الجرثومية، UCHL1 + الحيوانات المنوية، SYCP3 + الخلايا المنوية، CREM+ الحيوانات المنوية، SOX9+ خلايا سيرتولي. كما تم تحليل تكاثر (Ki67) وموت الخلايا المبرمج (CASPASE3) للخلايا الجرثومية وخلايا سيرتولي. تم تقييم نضج ...
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المؤلفون: Disi Bai, Fen Hu, Zhenfei Chen, Tianyang An, Xueliang Shang, Lu Yuan, Xiujun Zhang, Qingzhao Li, Yajing Chen
المصدر: International Journal of Nanomedicine. 15:3415-3431
مصطلحات موضوعية: Biophysics, Pharmaceutical Science, Bioengineering, 02 engineering and technology, Testicle, 010402 general chemistry, medicine.disease_cause, 01 natural sciences, Biomaterials, Andrology, Drug Discovery, medicine, Chemistry, Organic Chemistry, General Medicine, 021001 nanoscience & nanotechnology, Sperm, 0104 chemical sciences, medicine.anatomical_structure, Apoptosis, 0210 nano-technology, Luteinizing hormone, Reproductive toxicity, Spermatogenesis, Oxidative stress, Hormone
الوصف: Purpose Lanthanum oxide (La2O3) nanoparticles (NPs) have been widely used in catalytic and photoelectric applications , but the reproductive toxicity is still unclear. This study evaluated the reproductive toxicity of two different-sized La2O3 particles in the testes. Materials and methods Fifty Kunming mice were randomly divided into five groups. Mice were treated with La2O3 NPs by repeated intragastric administration for 90 days (control, nano-sized with 5, 10, 50 mg/ kg BW and micro-sized with 50 mg/ kg BW). Mice in the control group were treated with de-ionised water without La2O3 NPs. Sperm parameters, testicular histopathology, TEM assessment, hormone assay and nuclear factor erythroid 2-related factor 2 (Nrf-2) pathway were performed and evaluated. Results The body weight of mice treated with La2O3 NPs or not had no difference; sperm parameters and histological assessment showed that La2O3 NPs could induce reproductive toxicity in the testicle. Serum testosterone and gonadotropin-releasing hormone (GnRH) in the NH (nano-sized with 50 mg/ kg BW) group were markedly decreased relative to control group, and an increase of luteinizing hormone (LH) in NH group was detected . Additionally, transmission electron microscopy revealed that the ultrastructural abnormalities induced by La2O3 NPs were more severe than La2O3 MPs in the testes. Furthermore, La2O3 NPs treatment inhibited the translocation of nuclear factor erythroid 2-related factor 2 (Nrf-2) from the cytoplasm into the nucleus as well as the expression of downstream genes NAD(P)H quinone oxidoreductase1 (NQO1), hemeoxygenase 1 (HO-1) and ( glutathione peroxidase) GSH-Px, thus abrogating Nrf-2-mediated defense mechanisms against oxidative stress. Conclusions The results of this study demonstrated that La2O3 NPs improved the spermatogenesis defects in mice. La2O3 NPs inhibited Nrf-2/ARE signaling pathway that resulted in apoptosis in the mice testes.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::e4fa66c848bcd03ad12338d90a040e3bTest
https://doi.org/10.2147/ijn.s230949Test -
3
المؤلفون: Maryam Moghimian, Alireza Alihemmati, Hamed Shoorei, Seyed-Hosein Abtahi-Eivary, Amir Afshin Khaki, Majid Shokoohi, Arash Khaki
المصدر: Human & Experimental Toxicology. 39:1019-1030
مصطلحات موضوعية: Male, 0301 basic medicine, endocrine system, medicine.medical_specialty, Health, Toxicology and Mutagenesis, medicine.medical_treatment, Testicle, Biology, Steroidogenic Factor 1, Toxicology, Diabetes Mellitus, Experimental, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, Testis, medicine, Animals, Insulin, Testosterone, RNA, Messenger, Rats, Wistar, 030219 obstetrics & reproductive medicine, General Medicine, Luteinizing Hormone, Receptors, LH, Sertoli cell, Diabetes Mellitus, Type 1, 030104 developmental biology, medicine.anatomical_structure, Endocrinology, Hormone receptor, Cymenes, Receptors, FSH, Follicle Stimulating Hormone, Luteinizing hormone, Spermatogenesis, Hormone
الوصف: Diabetes mellitus (DM) is a complex metabolic disease and it is also closely associated with a reduction in fertility in male patients. The purpose of the present study was to investigate the antidiabetic effect of carvacrol (CRV), as a potent antioxidant, on the numbers of germ cells and Sertoli cells in testicular tissue, and the messenger RNA (mRNA) and protein expression of some genes involved in spermatogenesis, including luteinizing hormone/choriogonadotropin receptor ( LHCGR), follicle-stimulating hormone receptor ( FSHR), and steroidogenic factor 1 ( SF-1), as well as hormones such as luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone (T), and insulin. Adult male Wistar rats ( n = 32) were randomly divided into four groups (eight animals per group), including healthy control that received 0.2% Tween 80, diabetic control group, the diabetic group treated orally with CRV (75 mg/kg), and CRV group that received orally CRV (75 mg/kg). The duration of the treatment period lasted 8 weeks. In the diabetic group, the numbers of Sertoli cells and germ cells were significantly decreased, while the treatment with CRV prevented the degree of the damage to the cells mentioned earlier. CRV administration elevated the concentrations of insulin, T, FSH, and LH. Moreover, treatment with CRV significantly enhanced the levels of the mRNA and protein expression of SF-1, LHCGR, and FSHR. According to the obtained results, CRV administration could prevent the deleterious effects of DM on testicular germ cells, and it increases the levels of hormones and some essential genes, such as SF-1, LHCGR, and FSHR, involved in the process of spermatogenesis.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6cb176c19f0995bdc026b601d6f5f42eTest
https://doi.org/10.1177/0960327120909525Test -
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المؤلفون: J.A. Abecia, L. Pulinas, Philippe Chemineau, Melissa Carvajal-Serna, Matthieu Keller, Adriana Casao, J.A. Delgadillo, C. Palacios
المساهمون: University of Zaragoza - Universidad de Zaragoza [Zaragoza], Universidad de Salamanca, Università degli Studi di Sassari [Sassari] (UNISS), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Universidad Autónoma Agraria Antonio Narro (UAAAN), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
المصدر: Animal
Animal, Published by Elsevier (since 2021) / Cambridge University Press (until 2020), 2020, 14 (12), pp.2554-2561. ⟨10.1017/S1751731120001330⟩
Animal, Vol 14, Iss 12, Pp 2554-2561 (2020)
Zaguán: Repositorio Digital de la Universidad de Zaragoza
Universidad de Zaragoza
Zaguán. Repositorio Digital de la Universidad de Zaragoza
instname
animal
animal, Published by Elsevier (since 2021) / Cambridge University Press (until 2020), 2020, 14 (12), pp.2554-2561. ⟨10.1017/S1751731120001330⟩مصطلحات موضوعية: Male, sheep, Testicular volume, Live weight, socio-sexual signals, Biology, Testicle, SF1-1100, testicle, Sexual Behavior, Animal, 03 medical and health sciences, 0302 clinical medicine, Animal science, Estrus, anestrus, medicine, Animals, Testosterone, Ultrasonography, Estrous cycle, 030219 obstetrics & reproductive medicine, hormones, business.industry, 0402 animal and dairy science, Echogenicity, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, 04 agricultural and veterinary sciences, Luteinizing Hormone, Lh pulsatility, 040201 dairy & animal science, Animal culture, medicine.anatomical_structure, Female, Animal Science and Zoology, Seasons, business
الوصف: International audience; The continuous presence of active male small ruminants prevents seasonal anestrus in females, but evidence of the same mechanism operating from the females to the males is scarce. This study assessed the effects of the continuous presence of ewes in estrus in spring on ram sexual activity, testicular size and echogenicity, and LH and testosterone concentrations. On 1 March, 20 rams were assigned to two groups (n = 10 each): isolated (ISO) from other sheep, or stimulated (STI) by 12 ewes, which were separated from the rams by an openwork metal barrier, allowing contact between sexes. Each week, four ewes were induced into estrus by intravaginal sponges. Live weight, scrotal circumference, testicular width (TW) and length (TL) were recorded at the beginning and at the end of the experiment, and testicular volume (TV) was calculated; at the same time, testicular ultrasonography and color Doppler scanning were performed. Blood samples (March to May) were collected once per week for testosterone determinations, and at the end of the experiment, blood samples were collected for 6 h at 20-min intervals for LH analysis. Rams were exposed to four estrous ewes in a serving-capacity test. Scrotal circumference, TW and TL were higher in the STI than in the ISO rams (P < 0.05) in May, and TV was higher (P < 0.05) in the STI (391 +/- 17 cm(3)) than in the ISO rams (354 +/- 24 cm(3)). In ISO rams, the number of white pixels was higher (P < 0.01) in May (348 +/- 74) than in March (94 +/- 21) and differed significantly (P < 0.01) from that of the STI rams in May (160 +/- 33). In ISO rams, the number of grey pixels was higher (P < 0.05) in May (107 +/- 3) than it was in March (99 +/- 1). Stimulated and ISO rams did not differ significantly in mean LH plasma concentrations (0.8 +/- 0.5 v. 0.9 +/- 0.4 ng/ml), LH pulses (2.1 +/- 0.5 v. 2.2 +/- 0.2) and amplitude (2.0 +/- 0.4 v. 3.2 +/- 0.7 ng/ml, respectively). Stimulated rams had significantly higher testosterone concentrations than ISO rams from April to the end of the experiment. Stimulated rams performed more (P < 0.05) mountings with intromission (3.0 +/- 0.4) than did ISO rams (1.5 +/- 0.5). In conclusion, after 3 months in the continuous presence of ewes in estrus in spring, rams had higher TV and some testicular echogenic parameters were modified than isolated rams. Although exposed rams also had higher levels of testosterone after 2 months in the presence of estrous ewes, their LH pulsatility at the end of the study was not modified.
وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57808fcb3f1b1f5f9e69eb35ca395f49Test
https://hal.inrae.fr/hal-03500768Test -
5
المؤلفون: Manal M.S. Mansoury, Hala H.A. Khattab
المصدر: Andrologia. 52
مصطلحات موضوعية: Male, endocrine system, medicine.medical_specialty, Urology, 030232 urology & nephrology, Testicle, medicine.disease_cause, Testicular Diseases, Antioxidants, Lipid peroxidation, Superoxide dismutase, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Endocrinology, Internal medicine, Testis, medicine, Animals, Humans, Testosterone, Rats, Wistar, chemistry.chemical_classification, 030219 obstetrics & reproductive medicine, biology, Plant Extracts, business.industry, Glutathione peroxidase, Costus, General Medicine, Glutathione, Luteinizing Hormone, Spermatozoa, Sperm, Rats, Plant Leaves, Disease Models, Animal, Oxidative Stress, medicine.anatomical_structure, chemistry, Cyclosporine, biology.protein, Lipid Peroxidation, business, Oxidative stress
الوصف: Cyclosporine A is one of the most widely used drugs in organ transplant and oncology patients. But its use is accompanied by many toxicities. This study aimed to investigate the possible protective effect of Costus afer (C. afer) leaf extract on cyclosporine A-induced testicular toxicity. This study was carried out on 40 adult male Wistar rats were divided into four groups: control, C. afer, cyclosporine A and cyclosporine A+ C. afer groups. The investigations include genital weight, sperm count and characters, serum luteinising hormone (LH) and testosterone, testicular tissue contents of reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx) and lipid peroxidation (MDA). Besides, a histopathological examination of testicular tissue stained with haematoxylin and eosin (H & E) was performed. Cyclosporine A+ C. afer group showed a significant increase in the genital weight, serum testosterone, sperm count, motility and viability. Besides, the extract significantly decreased testicular content of MDA and increased SOD, CAT and GSHPx. C. afer coadministration significantly decreased serum LH and sperm abnormalities and protected against testicular histopathological alterations. The extract showed a protective effect against testicular toxicity associated with cyclosporine A and that was through an antioxidant mechanism.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22e1ff40d6d45323ce3e07dcabca2711Test
https://doi.org/10.1111/and.13561Test -
6
المصدر: AACE Clinical Case Reports, Vol 2, Iss 4, Pp e363-e366 (2016)
مصطلحات موضوعية: endocrine system, medicine.medical_specialty, Pathology, medicine.drug_class, 030232 urology & nephrology, Lymph node biopsy, Testicle, Diseases of the endocrine glands. Clinical endocrinology, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, Medicine, Orchiectomy, Testosterone, medicine.diagnostic_test, business.industry, Cancer, General Medicine, Hyperplasia, RC648-665, medicine.disease, Endocrinology, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Gonadotropin, business, Luteinizing hormone
الوصف: Objective: Embryonal cell carcinoma of the testicle with markedly elevated β-human chorionic gonadotropin (β-HCG) and testosterone concentrations with a paradoxical absence of secondary sexual characteristics has not been previously reported.Methods: A patient with a history of type 1 diabetes reported low libido and absent secondary sexual characteristics. A left testicular mass was discovered on examination. Further work up revealed high testosterone levels, suppressed luteinizing hormone and follicle-stimulating hormone levels, and erythrocytosis. Imaging studies revealed a 1-cm heavily calcified conglomerate mass in the left testicle and metastatic cancer involving axillary, supraclavicular, mediastinal, retroperitoneal, and pelvic lymph nodes.Results: Lymph node biopsy and surgical left orchiectomy specimens confirmed embryonal cell carcinoma with hyperplasia of Leydig cells. The patient was treated preoperatively with chemotherapy. Polycythemia and secondary sexual characteristics were restored after treatment.Conclusion: Our case demonstrates for the first time that an embryonal cell carcinoma of the testicle can be associated with polycythemia and markedly elevated β-HCG and testosterone concentrations with a paradoxical absence of secondary sexual characteristics. The secondary sexual characteristics can be restored and erythrocytosis reversed after normalization of β-HCG and testosterone concentrations.Abbreviations: DHT = dihydrotestosterone; FSH = follicle-stimulating hormone; HCG = human chorionic gonadotropin; LH = luteinizing hormone; T1D = type 1 diabetes mellitus
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9157f6fb2585f799a6c011fd1a085dffTest
https://doi.org/10.4158/ep151069.crTest -
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المؤلفون: Ke Zhang, Wenji Lu, Hao He, Xiaoqian Sun, Yong Wang, Jianteng Dong, Peng-Fei Tu, Yong Jiang, Qingqing Song, Qixin Wang, Chun Li
المصدر: Journal of Ethnopharmacology. 251:112500
مصطلحات موضوعية: Male, Testosterone propionate, endocrine system, medicine.medical_specialty, Cistanche, 17-Hydroxysteroid Dehydrogenases, Hydrocortisone, Biology, Testicle, Protective Agents, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Seminal vesicle, Internal medicine, Testis, Drug Discovery, medicine, Animals, Testosterone, Cholesterol Side-Chain Cleavage Enzyme, Glycosides, 030304 developmental biology, Pharmacology, Mice, Inbred ICR, 0303 health sciences, Reproduction, Steroidogenic acute regulatory protein, Luteinizing Hormone, Phenylethyl Alcohol, Phosphoproteins, Epididymis, Cistanche tubulosa, Endocrinology, medicine.anatomical_structure, chemistry, 030220 oncology & carcinogenesis, Luteinizing hormone, Signal Transduction
الوصف: Cistanche tubulosa (Schenk) R. Wight has been used frequently in traditional folk medicine for treatment of male sexual dysfunction (MSD). Phenylethanol glycosides, the main components of C. tubulosa, possess a variety of pharmacological activities due to their multiple properties. However, the underlying mechanism by which phenylethanol glycosides from C. tubulosa (CPhGs) regulates testicular steroids has not been elucidated to date.This study is to determine whether CPhGs promotes the reproductive functions of mice through CYP450-3β-HSD pathway of testosterone synthesis.The major compositions of C. tubulosa (CPhGs) were quantified by high performance liquid chromatography (HPLC). The model of reproductive injury in mice were induced by injection of hydrocortisone (HCT). Different doses of CPhGs (72, 145 and 289 mg/kg) and testosterone propionate (TP, positive control drug) were administrated intragastrically for 14 d. The reproductive functions (erectile incubation period, capture and ejaculation incubation period, number of captures and ejaculations) and organ weights (testicle, epididymis, seminal vesicle and penis) were then determined. The levels of luteinizing hormone and testosterone in serum were quantified by radioimmunoassay. The key enzymes in testosterone synthesis pathways such as steroidogenic acute regulatory protein (StAR), cytochrome P450 cholesterol side chain cleavage enzyme (P450scc/CYP11A1) and 3β-hydroxysteroid dehydrogenase (3β-HSD) in the testis were assessed by immunofluorescence (IF) staining or/and Western blot (WB) analysis.The results illustrated that the low dose of CPhGs (72 mg/kg) had no significant protective effect against the reproductive injury caused by HCT, while the moderate dose of CPhGs (145 mg/kg) improved the damaged reproductive ability and the declined levels of luteinizing hormone and testosterone in the model mice (P 0.001, P 0.05, respectively). In particular, high dose of CPhGs (289 mg/kg) was most effective in improving HCT-induced changes in body weight (P 0.01), reducing the incubation period of the erectile (P 0.001), capture (P 0.05) and ejaculation (P 0.01), and increasing the number of captures and ejaculations (P 0.01, P 0.05, respectively). The weights of testcle, epididymis, seminal vesicle and penis (P 0.001, P 0.01, P 0.01, P 0.001, respectively) were improved by high dose of CPhGs. The levels of testosterone and its upstream luteinizing hormone were up-regulated by high dose of CPhGs (P 0.001). Meanwhile, the expressions of the key steroidogenic enzymes including CYP11A1 and 3β-HSD were significantly up-regulated after CPhGs treatment (P 0.001), demonstrated that CPhGs exerted the effect through enhancing testosterone biosynthesis via CYP450-3β-HSD pathway.CPhGs could significantly protect against HCT-induced deleterious reproductive dysfunction and testis injury. The protective effects were exerted by up-regulating synthesis of testosterone via the CYP450-3β-HSD pathway in Leydig cells.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0291c35d8e9364bc571b5294e23ef60bTest
https://doi.org/10.1016/j.jep.2019.112500Test -
8
المؤلفون: M.M. Vogelsang, Clay A. Cavinder, Kathrin A. Dunlap, Peter C. Rakestraw, Raul Valdez, C.C. Love, J. D. McCormick
المصدر: The Professional Animal Scientist. 30:637-642
مصطلحات موضوعية: medicine.medical_specialty, media_common.quotation_subject, Testicle, chemistry.chemical_compound, medicine.anatomical_structure, Endocrinology, chemistry, Estrone sulfate, Internal medicine, Blood plasma, medicine, Animal Science and Zoology, Reproduction, Luteinizing hormone, Testosterone, Food Science, Hormonal response, media_common
الوصف: Hemicastration is a veterinary surgical procedure in stallions and may be needed for the removal of a diseased or damaged testicle. The effects of hemicastration on the neuroendocrine system and the hormonal response of the remaining testicle is unclear. Therefore, blood plasma concentrations of testosterone, luteinizing hormone, and estrone sulfate were assessed following hemicastration. Miniature stallions (n = 8) were used, and blood was drawn 7 d before hemicastration and 12 h, 48 h, 14 d, 30 d, and 90 d after hemicastration. Blood samples from all stallions were drawn every 15 min (T0, T15, T30, T45, T60 min) for 60 min each sampling period. Plasma luteinizing-hormone concentrations at 12 h, 48 h, 14 d, and 90 d were greater (P
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::c15e375c9fa4002370492df3d4da6a89Test
https://doi.org/10.15232/pas.2014-01340Test -
9
المؤلفون: Slobodan Stojanović, Gordana Uscebrka, Marina Štukelj, Dragan Zikic
المصدر: Acta Scientiae Veterinariae; v. 45 (2017): ARTICLES; 7
مصطلحات موضوعية: Germinal epithelium, endocrine system, BOAR, Boar taint, 040301 veterinary sciences, 0402 animal and dairy science, Lumen (anatomy), 04 agricultural and veterinary sciences, General Medicine, Biology, Testicle, pig, immunocastration, testicular morphology, 040201 dairy & animal science, 0403 veterinary science, Andrology, Seminiferous tubule, medicine.anatomical_structure, medicine, Luteinizing hormone, Spermatogenesis
الوصف: Background: Surgical castration of male piglets is frequently performed to prevent development of an unpleasant taste and flavor in meat of mature males, called boar taint. Disadvantages of this method are primarily related to the lower feed efficiency of surgically castrated male pigs, production of less lean meat and more fat on the carcasses than boars, as well as ethical issues associated with the procedure itself. Immunocastration is a method of immunization against gonadotropinreleasing hormone (GnRH) and represents an alternative to avoid boar taint. This study aimed to examine the influence of immunocastration on histological and morphometric parameters of male pig testes.Materials, Methods & Results: A total of 60 crossbred male pigs selected from 30 litters were assigned to two experimental groups: boars (n = 30) and immunocastrated males (n = 30). Two doses (2 mL per dose) of the Improvac® vaccine were given to pigs scheduled for immunocastration. The first injection was given at 10 weeks of age, and the second injection at 20 weeks of age. All pigs were fed the same commercial diet ad libitum, group-housed (15 pigs/pen) and slaughtered at 25 weeks of age. After slaughter, testicle weights were measured and samples of testicular tissue were taken for histological examination. Immunocastrated males had lower testicle weights than boars (P < 0.01). Furthermore, structural damage to the germinal epithelium was visible in the seminiferous tubules of vaccinated pigs. In the testicular interstitium of these animals, the number of Leydig cells were reduced; almost all of these cells exhibited different degrees of abnormality. Moreover, the diameter of the seminiferous tubules and thickness of the germinal epithelium were greater (P < 0.01), and the percentage of germinal epithelium in the seminiferous tubules as well as percentage of blood vessels in the interstitium were higher in boars as compared to immunocastrated males (P < 0.01). Seminiferous tubule lumen diameter was greater (P < 0.01), and percentage of lumen in the seminiferous tubules as well as percentage of non-blood vessel tissue in the interstitium were higher in immunocastrated males than boars (P < 0.01).Discussion: Vaccination against GnRH, as used in this study, reduced the synthesis of follicle-stimulating hormone (FSH) and luteinizing hormone (LH), which led to decreased spermatogenesis and androgen production. The smaller diameter of the seminiferous tubules as well as germinal epithelium thickness, and lower percentage of germinal epithelium in the seminiferous tubules in immunocastrated males could be explained by reduced FSH synthesis, and subsequent reduced spermatogenesis. The described testicular malformations in immunocastrated animals caused their lower weight, and greater diameter of the lumen as well as a higher lumen percentage of the seminiferous tubules. The vaccine reduced LH synthesis, so the Leydig cells of immunocastrated animals had lower activity than the same cells in boars. The percentage of blood vessels in the interstitium of boar testes was higher, which was related to the blood supply required for normal function of Leydig cells. It was concluded that differences existed in the structure of the seminiferous tubules and interstitium between boars and immunocastrated males , which were reflected in values of the examined histological and morphometric parameters. The development of these differences was related to the inhibition of GnRH synthesis and secretion.
وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4538b8eb7a72403d28893168514ad7ffTest
https://seer.ufrgs.br/ActaScientiaeVeterinariae/article/view/80582Test -
10
المؤلفون: Gozde Erkanli Senturk, Signem Eyuboglu, Hatice Akkaya, Bayram Yilmaz
المصدر: Journal of biochemical and molecular toxicology. 31(5)
مصطلحات موضوعية: 0301 basic medicine, Male, medicine.medical_specialty, Health, Toxicology and Mutagenesis, Protein degradation, Testicle, Toxicology, medicine.disease_cause, Biochemistry, Gene Expression Regulation, Enzymologic, Lipid peroxidation, Superoxide dismutase, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Kisspeptin, Methionine, Internal medicine, Testis, medicine, Animals, Rats, Wistar, Molecular Biology, Kisspeptins, biology, Superoxide Dismutase, General Medicine, Luteinizing Hormone, Catalase, Rats, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, chemistry, biology.protein, Molecular Medicine, Lipid Peroxidation, hormones, hormone substitutes, and hormone antagonists, 030217 neurology & neurosurgery, Oxidative stress
الوصف: Disruption of the balance oxidants, antioxidants cause various pathophysiological conditions such as lipid peroxidation, protein degradation, or DNA damage. We have examined possible effects of kisspeptin-10 on the structural damage produced by methionine-induced lipid peroxidation in testicle tissue of young rats. Kisspeptin-10 did not significantly affect spermatogenic cells in seminiferous tubules. Testosterone levels decreased in the methionine group as compared with the control group but without statistical significance. Luteinizing hormone levels decreased in the methionine group as compared with the control group (P
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::97e6d82caf1f99104b62e714352bab71Test
https://pubmed.ncbi.nlm.nih.gov/27900820Test
