التفاصيل البيبلوغرافية
| العنوان: |
Determination of reliable lung function parameters in intubated mice. |
| المؤلفون: |
Bonnardel, Eline1,2 (AUTHOR), Prevel, Renaud1,2 (AUTHOR), Campagnac, Marilyne1,2 (AUTHOR), Dubreuil, Marielle1,2 (AUTHOR), Marthan, Roger1,2,3 (AUTHOR), Berger, Patrick1,2,3 (AUTHOR), Dupin, Isabelle1,2 (AUTHOR) isabelle.dupin@u-bordeaux.fr |
| المصدر: |
Respiratory Research. 9/14/2019, Vol. 20 Issue 1, pN.PAG-N.PAG. 1p. 9 Graphs. |
| مصطلحات موضوعية: |
*RESPIRATORY organs, *LUNGS, *MICE, *RESPIRATORY diseases, *LONGITUDINAL method, *ANIMALS, *ASTHMA, *ATMOSPHERIC pressure, *OBSTRUCTIVE lung diseases, *LUNG physiology, *RESPIRATORY measurements, *PULMONARY function tests, *TRACHEA intubation, *TRACHEOTOMY, *PILOT projects, RESEARCH evaluation |
| مستخلص: |
Background: Animal models and, in particular, mice models, are important tools to investigate the pathogenesis of respiratory diseases and to test potential new therapeutic drugs. Lung function measurement is a key step in such investigation. In mice, it is usually performed using forced oscillation technique (FOT), negative pressure-driven forced expiratory (NPFE) and pressure-volume (PV) curve maneuvers. However, these techniques require a tracheostomy, which therefore only allows end-point measurements. Orotracheal intubation has been reported to be feasible and to give reproducible lung function measurements, but the agreement between intubation and tracheostomy generated-data remains to be tested.Methods: Using the Flexivent system, we measured lung function parameters (in particular, forced vital capacity (FVC), forced expiratory volume in the first 0.1 s (FEV0.1), compliance (Crs) of the respiratory system, compliance (C) measured using PV loop and an estimate of inspiratory capacity (A)) in healthy intubated BALB/cJ mice and C57BL/6 J mice and compared the results with similar measurements performed in the same mice subsequently tracheostomized after intubation, by means of paired comparison method, correlation and Bland-Altman analysis. The feasibility of repetitive lung function measurements by intubation was also tested.Results: We identified parameters that are accurately evaluated in intubated animals (i.e., FVC, FEV0.1, Crs, C and A in BALB/cJ and FVC, FEV0.1, and A in C57BL/6 J). Repetitive lung function measurements were obtained in C57BL/6 J mice.Conclusion: This subset of lung function parameters in orotracheally intubated mice is reliable, thereby allowing relevant longitudinal studies. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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