المؤلفون: Zainul Amiruddin Zakaria, Md. Atiar Rahman, Salama Mostafa Aboelenin, Md. Nazim Uddin, Afnan M. Alnajeebi, Nouf Abubakr Babteen

المصدر: BioMed Research International, Vol 2021 (2021)
BioMed Research International

مصطلحات موضوعية: Male, Article Subject, Blood sugar, Network Pharmacology, Pharmacology, Antioxidants, General Biochemistry, Genetics and Molecular Biology, Diabetes Mellitus, Experimental, Superoxide dismutase, chemistry.chemical_compound, Blood serum, In vivo, Lactate dehydrogenase, Animals, Hypoglycemic Agents, Aspartate Aminotransferases, Rats, Wistar, Oleanolic acid, chemistry.chemical_classification, Glutathione Peroxidase, General Immunology and Microbiology, biology, Plant Extracts, Superoxide Dismutase, Chemistry, Glutathione peroxidase, Alanine Transaminase, General Medicine, NFE2L2, Rats, Oxidative Stress, Liver, Vitaceae, biology.protein, Medicine, Medicine, Traditional, Research Article

الوصف: Background. Hatikana is a traditional medicinal plant used to treat inflammation, urolithiasis, goiter, cancer, wounds and sores, gastrointestinal, tumor, tetanus, arthritis, hepatic damage, neurodegeneration, and other ailments. The goal of this study is to investigate the antidiabetic properties of Hatikana extract (HKEx) and to construct the effects of its natural constituents on the genes and biochemical indices that are connected with them. Methods. HKEx was evaluated using GC-MS and undertaken for a three-week intervention in fructose-fed STZ-induced Wistar albino rats at the doses of HKEx50, HKEx100, and HKEx200 mg/kg bw. Following intervention, blood serum was examined for biochemical markers, and liver tissue was investigated for the mRNA expression of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD1) by RTPCR analysis. Most abundant compounds (oleanolic acid, 7α, 28-olean diol, and stigmasterol) from GC-MS were chosen for the network pharmacological assay to verify function-specific gene-compound interactions using STITCH, STRING, GSEA, and Cytoscape plugin cytoHubba. Results. In vivo results showed a significant ( P < 0.05 ) decrease of blood sugar, aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine kinase (CK-MB), and lactate dehydrogenase (LDH) and increase of liver glycogen, glucose load, and serum insulin. Out of three antioxidative genes, catalase (CAT) and superoxide dismutase (SOD1) were found to be few fold increased. Oleanolic acid and stigmasterol were noticed to strongly interact with 27 target proteins. Oleanolic acid interacted with the proteins AKR1B10, CASP3, CASP8, CYP1A2, CYP1A2, HMGB1, NAMPT, NFE2L2, NQO1, PPARA, PTGIR, TOP1, TOP2A, UGT2B10, and UGT2B11 and stigmasterol with ABCA1, ABCG5, ABCG8, CTSE, HMGCR, IL10, CXCL8, NR1H2, NR1H3, SLCO1B1, SREBF2, and TNF. Protein-protein interaction (PPI) analysis revealed the involvement of 25 target proteins out of twenty seven. Cytoscape plugin cytoHubba identified TNF, CXCL8, CASP3, PPARA, SREBF2, and IL10 as top hub genes. Pathway analysis identified 31 KEGG metabolic, signaling, and immunogenic pathways associated with diabetes. Notable degree of PPI enrichment showed that SOD1 and CAT are responsible for controlling signaling networks and enriched pathways. Conclusion. The findings show that antioxidative genes have regulatory potential, allowing the HKEx to be employed as a possible antidiabetic source pending further validation.

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الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::171b1443e18d6abbdae6428f61004962Test
https://doi.org/10.1155/2021/6978450Test

المؤلفون: Moncef Fekih, Emna Gaïes, Hammami Mohamed Bassem, Ridha Ben Ali, Michelle-Véronique Elmay, Israa Dahmani, Sihem El Aïdli, Dorra Ben Said, Achraf Chedly

المصدر: Drug and Chemical Toxicology. 45:2594-2600

مصطلحات موضوعية: Drug, Health, Toxicology and Mutagenesis, media_common.quotation_subject, medicine.medical_treatment, Antitubercular Agents, Pharmacology, Toxicology, chemistry.chemical_compound, Lactate dehydrogenase, Isoniazid, Animals, Medicine, Aspartate Aminotransferases, Rats, Wistar, Alanine aminotransferase, Lactate Dehydrogenases, Saline, media_common, Liver injury, Chemical Health and Safety, business.industry, Public Health, Environmental and Occupational Health, Alanine Transaminase, General Medicine, Alkaline Phosphatase, bacterial infections and mycoses, medicine.disease, Lipids, Rats, Liver, chemistry, Toxicity, Alkaline phosphatase, Chemical and Drug Induced Liver Injury, business, medicine.drug

الوصف: Isoniazid (INH), being the first-line drug used as an anti-tuberculosis drug, is known to be associated with physiological deteriorations including hepatic and neurologic disturbances. This study was aimed at biochemical and behavioral characterization of toxic manifestations of isoniazid treatment in Wistar rats. Experimental animals were divided into four groups. Each group consists of six animals including the control group (saline solution), I25 group (25 mg/kg of INH), I50 group (50 mg/kg of INH), and I100 group (100 mg/kg of INH). Animals received daily INH for 30 days. Isoniazid is known to be associated with hepatotoxicity; it's among the most common causes of drug-induced toxicities. For this reason assays for aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) were performed to assess liver toxicity. Moreover, behavioral study, renal, and lipid parameters were also assessed in addition to histological features of the liver and brain. Significant differences in all studied parameters were seen especially in the I100 group and a marked increase in liver enzymes activities, such as AST and ALT was observed. In another hand, there were no major clinical signs in treated animals, except fatigue and anxiety in the I100 group. On the other hand, the histological findings showed potential liver and brain injury which was evidenced by degenerative changes, infiltration, and hepatocyte necrosis, in addition to the appearance of many pyramidales cells in the gyrus. The current study findings suggest that INH interacts with multiple biochemical pathways in the body what comes up by behavioral changes and liver disturbances in animals caused by INH toxicity.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73e31895e57f61027548fdda7d3f7296Test
https://doi.org/10.1080/01480545.2021.1979029Test

المؤلفون: Sabrina Ait Abderrahim, Nawel Ait Abderrahim, Baker Al-Shara, Mohamed Boussaid, Khaled Taïbi, Leila Ait Abderrahim

المصدر: Toxicon. 200:30-37

مصطلحات موضوعية: Antioxidant, Microcystins, Sorbitol dehydrogenase, medicine.medical_treatment, food and beverages, Microcystin-LR, Glutathione, Pharmacology, Toxicology, medicine.disease_cause, Antioxidants, Lipid peroxidation, Mice, Oxidative Stress, chemistry.chemical_compound, Liver, chemistry, Lactate dehydrogenase, Toxicity, medicine, Animals, Marine Toxins, Garlic, Oxidative stress

الوصف: Microcystins (MCs) are hepatotoxic cyanotoxins implicated in several incidents of human and animal toxicity. Microcystin-(Lysine, Arginine) or MC-LR is the most toxic and encountered variant of MCs where oxidative stress plays a key role in its toxicity. This study investigated the oxidative damages induced in the liver and heart of Balb/C mice by an intraperitoneal injected acute dose of MC-LR. Thereafter, the potential protective effect of garlic (Allium sativum) extract supplementation against such damages was assessed through the evaluation of oxidative stress and cytotoxicity markers. Lipid peroxidation (LPO), carbonyl content (CC), glutathione content (GSH), alkaline phosphatase activity (ALP), lactate dehydrogenase (LDH) and sorbitol dehydrogenase (SDH) activities were measured. Results showed important oxidative damages in hepatic and cardiac cells of mice injected with the toxin. However, these damages have been significantly reduced in mice supplemented with garlic extract. Thus, this study demonstrated for the first time the effective use of garlic as an antioxidant agent against oxidative damages induced by MC-LR. As well, this study supports the use of garlic as a potential remedy against pathologies related to toxic agents.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f915764791b9f98fb58ede8946dd5721Test
https://doi.org/10.1016/j.toxicon.2021.06.018Test

المؤلفون: Myrene R. D’souza, Khalid Mohammed Naji, Bushra Y. Al-Khatib, Nora Saif Al-Haj

المصدر: BMC Pharmacology and Toxicology, Vol 22, Iss 1, Pp 1-11 (2021)
BMC Pharmacology & Toxicology

مصطلحات موضوعية: 0301 basic medicine, Male, Bilirubin, medicine.medical_treatment, RM1-950, Pharmacology, Protective Agents, Melittin, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Lactate dehydrogenase, Malondialdehyde, RA1190-1270, medicine, Isoniazid, Animals, Tuberculosis, Pharmacology (medical), Aspartate Aminotransferases, Bee venom, Saline, Rifampicin, business.industry, Hepatotoxicity, Alanine Transaminase, Glutathione, Blood Proteins, Alkaline Phosphatase, Melitten, Rats, 030104 developmental biology, chemistry, Liver, 030220 oncology & carcinogenesis, Toxicology. Poisons, Alkaline phosphatase, Therapeutics. Pharmacology, Chemical and Drug Induced Liver Injury, Rifampin, business, medicine.drug, Research Article

الوصف: BackgroundThe present study investigated the ameliorative effect of melittin, a major polypeptide in the venom of honeybee (Apis mellifera), on isoniazid-(INH) and rifampicin-(RIF) induced hepatotoxicity in male albino rats.MethodThirty rats (140-200 g) were divided into five groups (n = 6): normal control (NC) received normal saline orally (NaCl, 0.9%; toxic (T) group received INH + RIF (each rat received 100 mg/kg, p.o.); melittin (Mel15, Mel30) groups (each rat received 15 or 30 μg/kg s.c); and normal recovery (NR) group received INH + RIF (each rat received 100 mg/kg, p.o.). Blood and liver samples were collected for biochemical, hematological and histopathological studies respectively.ResultsThe administration of melittin was found to prevent the antitubercular drug-induced alterations in the diagnostic markers; reduced glutathione (GSH), direct bilirubin (DB), total bilirubin (TB), aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), and total serum protein (TSP). Besides, hematological alterations were significantly high in Mel groups when compared to the toxic group. The NR group exhibited lower levels of DB, TB, ALP, LDH and TSP. In addition, treatment with melittin offered protection in the NR group with respect to MDA levels.ConclusionEvidence from this study suggests that melittin is beneficial for the prevention of acute hepatic failure in antitubercular drug-induced hepatoxicity and could be used as a potential therapeutic agent.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf0dc8db1532b81b74f63f446338ebd8Test
https://doaj.org/article/bf86734e1c1e4ae18f70398a1043577fTest

المؤلفون: Volodymyr I. Lushchak, Nadiia Mosiichuk, Viktor V. Husak, Kenneth B. Storey

المصدر: Chemical research in toxicology. 34(12)

مصطلحات موضوعية: Alanine, Increased lactate dehydrogenase activity, biology, Glycogen, Dose-Response Relationship, Drug, Molecular Structure, General Medicine, Pharmacology, Pesticide, Triazoles, Toxicology, Fungicides, Industrial, Fungicide, chemistry.chemical_compound, Glucose, Alanine transaminase, chemistry, Liver, Lactate dehydrogenase, Goldfish, Toxicity, biology.protein, Animals

الوصف: Triazole fungicides are widely used in agriculture that leads to pollution of freshwater ecosystems. The mechanisms of toxicity to fish by the triazole fungicide Topas that contains penconazole (1-[2-(2,4-dichlorophenyl)pentyl]-1H-1,2,4-triazole) have not been studied. The present study aimed to evaluate the effect of goldfish exposure for 96 h to the fungicide Topas at concentrations of 1.5, 15, or 25 mg/L on the plasma and liver biochemical parameters and blood hematological profile. Goldfish exposure to Topas decreased alanine and aspartate transaminase activity and increased lactate dehydrogenase activity in the liver. Plasma lactate dehydrogenase and alanine transaminase activities were elevated in fungicide-treated fish. Topas exposure also enhanced plasma glucose and triacylglycerol concentrations. In the liver, fungicide treatment decreased levels of glucose but elevated triacylglycerols, glycogen, and protein. The results indicate that acute exposure of goldfish to Topas induced strong metabolic perturbations and disruptions of metabolic parameters, suggesting that these could be used to assess sublethal or acute toxic effects of pesticides on aquatic species.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b377887103c70e8b49f535b671f6f22Test
https://pubmed.ncbi.nlm.nih.gov/34793142Test

المؤلفون: Dong Ruoyao, Yasong Ding, Meng Shengnan, Shiqi Wang, Hongyun Wang, Lingdi Yin

المصدر: Pharmacology. 106:606-615

مصطلحات موضوعية: medicine.medical_specialty, NF-E2-Related Factor 2, Asparagine synthetase, Rats, Sprague-Dawley, chemistry.chemical_compound, Liver Function Tests, AMP-activated protein kinase, Internal medicine, Lactate dehydrogenase, Diabetes mellitus, medicine, Animals, Canagliflozin, Carbon Tetrachloride, Sodium-Glucose Transporter 2 Inhibitors, Pharmacology, Liver injury, biology, business.industry, Adenylate Kinase, AMPK, Aspartate-Ammonia Ligase, General Medicine, medicine.disease, Activating Transcription Factor 4, Rats, Disease Models, Animal, Endocrinology, Liver, chemistry, Carbon tetrachloride, biology.protein, Liver function, Chemical and Drug Induced Liver Injury, business

الوصف: Introduction: Canagliflozin (CANA) is a sodium-glucose cotransporter 2 inhibitor that was recently approved for treating diabetes. However, its effects on liver function are not well understood. The function of asparagine synthetase (ASNS) has been studied in several cancers but not in liver injury. Therefore, we investigated the connection between CANA and ASNS in alleviating damage (i.e., their hepatoprotective effect) in a rat liver injury model. Methods: The rat model of liver injury was established using carbon tetrachloride treatment. Rats with liver injury were administered CANA orally for 8 weeks daily. After week 8, peripheral blood was collected to measure serum alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase levels. Liver histopathology was examined using hematoxylin and eosin staining to determine the degree of liver injury. Protein expression in the rat livers was examined using Western blotting. Results: CANA treatment decreased serum alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase levels compared with those of the untreated group, demonstrating diminished liver injury. Mechanistically, CANA treatment activated AMP-activated protein kinase (AMPK), leading to increased nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) and activating transcription factor 4 (ATF4), which upregulated ASNS expression in liver-injured rats. Conclusion: CANA significantly alleviated liver injury by activating the AMPK/Nrf2/ATF4 axis and upregulating ASNS expression, indicating its potential for treating patients with type 2 diabetes mellitus with impaired liver function.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::80026caec34e989580809335f6671343Test
https://doi.org/10.1159/000518492Test

المؤلفون: Renad Mammadov, Muhammet Ozer, Bahadir Suleyman, Abdulkadir Coban, Halis Suleyman, Sahin Lacin, Mustafa Özkaraca, Ali Veysel Kara, Fatih Ozcicek, Baran Akagunduz

المصدر: Experimental Animals

مصطلحات موضوعية: Male, Indazoles, Necrosis, Original, experimental models, Pharmacology, Protective Agents, medicine.disease_cause, General Biochemistry, Genetics and Molecular Biology, Hydropic degeneration, taxifolin, Pazopanib, chemistry.chemical_compound, Lactate dehydrogenase, pazopanib, medicine, Animals, oxidative stress, Taxifolin, Rats, Wistar, Sulfonamides, General Veterinary, Stomach, General Medicine, Malondialdehyde, medicine.disease, liver toxicity, Rats, Pyrimidines, medicine.anatomical_structure, Liver, chemistry, Quercetin, Animal Science and Zoology, Chemical and Drug Induced Liver Injury, medicine.symptom, Oxidative stress, medicine.drug

الوصف: Pazopanib is a tyrosine kinase inhibitor that is generally used for the treatment of metastatic renal cell cancer and advanced soft tissue sarcoma. It can cause various degrees of hepatotoxicity. Our study aimed to investigate the effect of taxifolin on pazopanib-induced liver toxicity. A total of 18 rats were divided into three groups: the pazopanib (PP), pazopanib plus taxifolin (TPP), and control (C) group. Taxifolin was administered to the TPP (n=6) group with a dose of 50 mg/kg. Distilled water was orally admnistered to the C (n=6) and PP (n=6) groups as a solvent. Subsequently, pazopanib 200 mg/kg was administered to the TPP and PP groups via the stomach. This procedure was repeated once a day for four weeks. Then, all rats were sacrificed, and their livers were removed. Malondialdehyde (MDA), total glutathione (tGSH), total oxidant status (TOS), and total antioxidant status (TAS) levels were evaluated. MDA and TOS levels were higher in the PP group compared with the levels of the other parameters (P

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::132fa6592797cfb0705b3d5e96453b37Test
https://doi.org/10.1538/expanim.20-0103Test

المؤلفون: Jaleh Varshosaz, Mina Mirian, Leila Safaeian, Setareh Jandaghian, Saeedeh Fardshouraki, Somayeh Taymouri

المصدر: Anti-Cancer Agents in Medicinal Chemistry. 20:1966-1980

مصطلحات موضوعية: Cancer Research, Cell Survival, Surface Properties, medicine.drug_class, medicine.medical_treatment, Intraperitoneal injection, Antineoplastic Agents, Capsules, 02 engineering and technology, Pharmacology, Tyrosine-kinase inhibitor, Cell Line, Structure-Activity Relationship, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Lactate dehydrogenase, medicine, Animals, Humans, MTT assay, Particle Size, Rats, Wistar, Protein Kinase Inhibitors, Cardiotoxicity, Dose-Response Relationship, Drug, Molecular Structure, Heart, Imatinib, 021001 nanoscience & nanotechnology, Rats, Liver, chemistry, 030220 oncology & carcinogenesis, Imatinib Mesylate, Nanoparticles, Molecular Medicine, Alkaline phosphatase, Female, Rituximab, 0210 nano-technology, Drug carrier, Porosity, medicine.drug

الوصف: Background: Using imatinib, a tyrosine kinase inhibitor drug used in lymphoblastic leukemia, has always had limitations due to its cardiotoxicity and hepatotoxicity side effects. The objective of this study is to develop a target-oriented drug carrier to minimize these adverse effects by the controlled release of the drug. Methods: KIT-5 nanoparticles were functionalized with 3-aminopropyltriethoxysilane and conjugated to rituximab as the targeting agent for the CD20 positive receptors of the B-cells. Then they were loaded with imatinib and their physical properties were characterized. The cell cytotoxicity of the nanoparticles was studied by MTT assay in Ramos (CD20 positive) and Jurkat cell lines (CD20 negative) and their cellular uptake was shown by fluorescence microscope. Wistar rats received an intraperitoneal injection of 50 mg/kg of the free drug or targeted nanoparticles for 21 days. Then the level of aspartate Aminotransferase (AST), alanine Aminotransferase (ALT), Alkaline Phosphatase (ALP) and Lactate Dehydrogenase (LDH) were measured in serum of animals. The cardiotoxicity and hepatotoxicity of the drug were also studied by hematoxylin and eosin staining of the tissues. Results: The targeted nanoparticles of imatinib showed to be more cytotoxic to Ramos cells rather than Jurkat cells. The results of the biochemical analysis displayed a significant reduction in AST, ALT, ALP, and LDH levels in animals treated with targeted nanoparticles, compared to the free drug group. By comparison with the free imatinib, histopathological results represented less cardiotoxicity and hepatotoxicity in the animals, which received the drug through the current designed delivery system. Conclusion: The obtained results confirmed that the rituximab targeted KIT-5 nanoparticles are promising in the controlled release of imatinib and could decrease its cardiotoxicity and hepatotoxicity side effects.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad1650b17b5d4551066bba4d642c52fdTest
https://doi.org/10.2174/1871520620666200619174323Test

المؤلفون: Fábio Erminio Mingatto, Paulo Francisco Veiga Bizerra, Anilda Rufino de Jesus Santos Guimarães, Camila Araújo Miranda

المساهمون: Universidade Estadual Paulista (UNESP), Universidade Estadual de Maringá (UEM)

المصدر: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP

مصطلحات موضوعية: Antioxidant, Cell Survival, Nitrogen, Health, Toxicology and Mutagenesis, Metabolite, medicine.medical_treatment, Cytotoxicity, Pharmacology, Toxicology, medicine.disease_cause, liver, Dithiothreitol, Neonicotinoids, chemistry.chemical_compound, Lactate dehydrogenase, medicine, Humans, oxidative stress, Viability assay, insecticide, Hep G2 Cells, Glutathione, Nitro Compounds, Reactive Nitrogen Species, Oxygen, Oxidative Stress, cell death, chemistry, Reactive Oxygen Species, Oxidative stress

الوصف: Made available in DSpace on 2022-05-01T10:02:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-01-01 Imidacloprid (IMD) is a neonicotinoid insecticide used in large quantities worldwide in both veterinary and agronomic applications. Several studies have shown adverse effects of IMD on non-target organisms, with the liver being identified as the main affected organ. This study aimed to evaluate the effects of IMD on human hepatoblastoma (HepG2) cells. HepG2 were exposed to IMD (0.25–2.0 mM) for 24 and 48 h. IMD treatment resulted in cytotoxicity in the HepG2, inhibiting cell proliferation in a dose- and time-dependent manner, starting at concentrations of 0.5 mM (24 h) and 0.25 mM (48 h), and reducing cell viability from 0.5 mM onwards (24 and 48 h). IMD significantly decreased the mitochondrial membrane potential at both time points investigated (2.0 mM), and also induced damage to the cell membrane, demonstrated by significant dose and time-dependent increases in lactate dehydrogenase (LDH) release from concentrations of 1.0 mM (24 h) and 0.5 mM (48 h) upwards. IMD treatment also increased the production of reactive oxygen and nitrogen species (ROS/RNS) at rates above 50% following 0.5 mM (24 h) or 0.25 mM (48 h) concentrations, and caused a significant decrease in reduced/oxidized glutathione ratio (GSH/GSSG), indicating oxidative stress. Furthermore, the antioxidant dithiothreitol, which reacts with ROS/RNS and acts as a thiol reducing agent, inhibited the cytotoxic effect of IMD. In addition, the metabolite IMD-olefin was more toxic than IMD. Our results indicate that IMD induces cytotoxicity in HepG2 cells and that this effect may be associated with an increase in the generation of ROS/RNS. Department of Animal Science College of Agricultural and Technological Sciences São Paulo State University (UNESP) Department of Biochemistry Maringá State University (UEM) Department of Animal Science College of Agricultural and Technological Sciences São Paulo State University (UNESP)

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::121b8592dfaa6bcbd82e45abc22dce1fTest

المؤلفون: Sobia Ahsan Halim, Nasiara Karim, Ajmal Khan, Haroon Khan, Najeeb Ur Rehman, Rehmat Gul, Ahmed Al-Harrasi, Sagheer Ahmed

المصدر: Biomedicine & Pharmacotherapy, Vol 143, Iss, Pp 112131-(2021)

مصطلحات موضوعية: Male, Bilirubin, Histological analysis, Anti-Inflammatory Agents, Aspartate transaminase, RM1-950, Pharmacology, Antioxidants, Superoxide dismutase, chemistry.chemical_compound, Mice, Blood serum, Lactate dehydrogenase, Medicine, Animals, Commiphora, Acetaminophen, biology, business.industry, General Medicine, Liver biomarkers, Myrrhanone B (MN), Triterpenes, Disease Models, Animal, Oxidative Stress, Myrrhanol B (ML), chemistry, Alanine transaminase, Liver, Oxidative biomarkers, biology.protein, Commipohora mukul, Alkaline phosphatase, Therapeutics. Pharmacology, Chemical and Drug Induced Liver Injury, business, Biomarkers, Resins, Plant, medicine.drug

الوصف: Despite a large number of liver disorders, clinically useful drugs are scarce. Moreover, the available therapies are facing the challenges of efficacy and safety. Commipohora mukul has been used in folk medicine globally for millennia for the treatment of several ailments. The current study was designed to evaluate the possible hepatoprotective activity of Myrrhanone B (MN) and Myrrhanol B (ML) isolated from C. mukul using an animal model. The animals (Swiss albino mice) were segregated into seven groups, each comprising six mice. The first group was treated with normal saline at a dose of 1 ML/kg daily intraperitoneally (i.p.) for one week. The second group was treated with acetaminophen (APAP) (250 mg/kg, i.p.), it was taken as a negative control. Group 3 was used as a positive control (treated with Silymarin (100 mg/kg, i.p.)). While groups 4–7 were used as experimental groups (termed as groups II to IV), which were treated with ML and MN at a dose of 0.6 mg/kg, and 1.2 mg/kg (i.p.) for one week. Subsequently, blood serum and liver tissue samples were collected for biochemical and histopathological analysis. Both compounds significantly improved the levels of liver biomarkers including aspartate transaminase (AST), alkaline phosphatase (ALP), bilirubin, lactate dehydrogenase (LDH), and alanine transaminase (ALT) as compared to the normal saline-treated group in APAP-induced hepatotoxic mice. Moreover, both compounds significantly modulated the expression of oxidative biomarkers including superoxide dismutase (SOD), reduced glutathione (GSH), and catalase (CAT) at the same doses. Additionally, ML and MN showed a remarkable improvement in histological changes with only mild inflammation, mild hemorrhage, no necrosis, and no pyknosis as compared to the control groups. In conclusion, MN and ML exhibited significant hepatoprotective effects in the animal model used in this study.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::358089a9c52a64b54908e3f56b083f47Test
http://www.sciencedirect.com/science/article/pii/S075333222100915XTest