التفاصيل البيبلوغرافية
| العنوان: |
Prostate cancer extracellular vesicle digital scoring assay – a rapid noninvasive approach for quantification of disease-relevant mRNAs. |
| المؤلفون: |
Wang, Jasmine J., Sun, Na, Lee, Yi-Te, Kim, Minhyung, Vagner, Tatyana, Rohena-Rivera, Krizia, Wang, Zhili, Chen, Zijing, Zhang, Ryan Y., Lee, Junseok, Zhang, Ceng, Tang, Hubert, Widjaja, Josephine, Zhang, Tiffany X., Qi, Dongping, Teng, Pai-Chi, Jan, Yu Jen, Hou, Kuan-Chu, Hamann, Candace, Sandler, Howard M. |
| المصدر: |
Nano Today; Feb2023, Vol. 48, pN.PAG-N.PAG, 1p |
| مصطلحات موضوعية: |
EXTRACELLULAR vesicles, PROSTATE cancer, RECEIVER operating characteristic curves, PRECIPITATION (Chemistry), CIRCULATING tumor DNA |
| مستخلص: |
Optimizing outcomes in prostate cancer (PCa) requires precision in characterization of disease status. This effort was directed at developing a PCa extracellular vesicle (EV) Digital Scoring Assay (DSA) for detecting metastasis and monitoring progression of PCa. PCa EV DSA is comprised of an EV purification device (i.e., EV Click Chip) and reverse-transcription droplet digital PCR that quantifies 11 PCa-relevant mRNA in purified PCa-derived EVs. A Met score was computed for each plasma sample based on the expression of the 11-gene panel using the weighted Z score method. Under optimized conditions, the EV Click Chips outperformed the ultracentrifugation or precipitation method of purifying PCa-derived EVs from artificial plasma samples. Using PCa EV DSA, the Met score distinguished metastatic (n = 20) from localized PCa (n = 20) with an area under the receiver operating characteristic curve of 0.88 (95% CI:0.78–0.98). Furthermore, longitudinal analysis of three PCa patients showed the dynamics of the Met scores reflected clinical behavior even when disease was undetectable by imaging. Overall, a sensitive PCa EV DSA was developed to identify metastatic PCa and reveal dynamic disease states noninvasively. This assay may complement current imaging tools and blood-based tests for timely detection of metastatic progression that can improve care for PCa patients. [Display omitted] • Prostate Ca (PCa)-derived extracellular vesicles (EV) were isolated via nanosurface. • This approach outperformed ultracentrifugation and precipitation. • An EV-mRNA signature could distinguish metastatic from localized PCa patients. • Changes in this mRNA signature reflected the dynamic clinical status of PCa patients. [ABSTRACT FROM AUTHOR] |
|
Copyright of Nano Today is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Supplemental Index |
