التفاصيل البيبلوغرافية
| العنوان: |
A functional group–guided approach to aptamers for small molecules. |
| المؤلفون: |
Yang, Kyungae1 ky2231@cumc.columbia.edu, Mitchell, Noelle M.2, Banerjee, Saswata1, Zhenzhuang Cheng1, Taylor, Steven1, Kostic, Aleksandra M.1, Wong, Isabel1, Sajjath, Sairaj1, Yameng Zhang1, Stevens, Jacob1, Mohan, Sumit3, Landry, Donald W.1, Worgall, Tilla S.4, Andrews, Anne M.5, Stojanovic, Milan N.1,6 mns18@cumc.columbia.edu |
| المصدر: |
Science. 6/2/2023, Vol. 380 Issue 6648, p942-948. 7p. 4 Color Photographs. |
| مصطلحات موضوعية: |
*SMALL molecules, *APTAMERS, *FUNCTIONAL groups, *VORICONAZOLE, *LEUCINE, *BIOSENSORS |
| مستخلص: |
Aptameric receptors are important biosensor components, yet our ability to identify them depends on the target structures. We analyzed the contributions of individual functional groups on small molecules to binding within 27 target-aptamer pairs, identifying potential hindrances to receptor isolation—for example, negative cooperativity between sterically hindered functional groups. To increase the probability of aptamer isolation for important targets, such as leucine and voriconazole, for which multiple previous selection attempts failed, we designed tailored strategies focused on overcoming individual structural barriers to successful selections. This approach enables us to move beyond standardized protocols into functional group–guided searches, relying on sequences common to receptors for targets and their analogs to serve as anchors in regions of vast oligonucleotide spaces wherein useful reagents are likely to be found. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |
