Rapid Detection of Pathogens in Wound Exudate via Nucleic Acid Lateral Flow Immunoassay
| العنوان: | Rapid Detection of Pathogens in Wound Exudate via Nucleic Acid Lateral Flow Immunoassay |
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| المؤلفون: | René D. Verboket, Susanna M. Früh, Daniel M. Kainz, Felix von Stetten, Anna Brunauer |
| المصدر: | Biosensors Volume 11 Issue 3 Biosensors, Vol 11, Iss 74, p 74 (2021) |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Point-of-Care Systems, lcsh:Biotechnology, paper-based detection, Clinical Biochemistry, wound exudate, Recombinase Polymerase Amplification, Biosensing Techniques, medicine.disease_cause, 01 natural sciences, Article, Microbiology, 03 medical and health sciences, Limit of Detection, Nucleic Acids, lcsh:TP248.13-248.65, ddc:570, medicine, Humans, Sample preparation, recombinase polymerase amplification, ddc:610, Pathogen, 030304 developmental biology, Immunoassay, Detection limit, 0303 health sciences, nucleic acid amplification test, Pseudomonas aeruginosa, Chemistry, Wound.exudate, 010401 analytical chemistry, Exudates and Transudates, nucleic acid lateral flow immunoassay, General Medicine, 0104 chemical sciences, Nucleic acid, Wounds and Injuries, wound infection, point-of-care diagnostics, Nucleic Acid Amplification Techniques, Lateral flow immunoassay |
| الوصف: | The rapid detection of pathogens in infected wounds can significantly improve the clinical outcome. Wound exudate, which can be collected in a non-invasive way, offers an attractive sample material for the detection of pathogens at the point-of-care (POC). Here, we report the development of a nucleic acid lateral flow immunoassay for direct detection of isothermally amplified DNA combined with fast sample preparation. The streamlined protocol was evaluated using human wound exudate spiked with the opportunistic pathogen Pseudomonas aeruginosa that cause severe health issues upon wound colonization. A detection limit of 2.1 × 105 CFU per mL of wound fluid was achieved, and no cross-reaction with other pathogens was observed. Furthermore, we integrated an internal amplification control that excludes false negative results and, in combination with the flow control, ensures the validity of the test result. The paper-based approach with only three simple hands-on steps has a turn-around time of less than 30 min and covers the complete analytical process chain from sample to answer. This newly developed workflow for wound fluid diagnostics has tremendous potential for reliable pathogen POC testing and subsequent target-oriented therapy. |
| وصف الملف: | application/octet-stream; application/pdf |
| تدمد: | 2079-6374 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2737464d4aa8dfc2b16bf2c3d27aee1bTest https://doi.org/10.3390/bios11030074Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2737464d4aa8dfc2b16bf2c3d27aee1b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20796374 |
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