دورية أكاديمية
Performance evaluation of different strategies based on microscopy techniques, rapid diagnostic test and molecular loop-mediated isothermal amplification assay for the diagnosis of imported malaria
| العنوان: | Performance evaluation of different strategies based on microscopy techniques, rapid diagnostic test and molecular loop-mediated isothermal amplification assay for the diagnosis of imported malaria |
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| المؤلفون: | Charpentier, E., Benichou, E., Pagès, A., Chauvin, P., Fillaux, J., Valentin, A., Guegan, H., Guemas, E., Salabert, A.-S., Armengol, C., Menard, S., Cassaing, S., Berry, A., Iriart, X. |
| المساهمون: | Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Centre de Physiopathologie Toulouse Purpan (CPTP), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Epidémiologie et analyses en santé publique : risques, maladies chroniques et handicaps (LEASP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Toulouse NeuroImaging Center (ToNIC), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Toulouse Mind & Brain Institut (TMBI), Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT) |
| المصدر: | ISSN: 1198-743X. |
| بيانات النشر: | HAL CCSD Elsevier for the European Society of Clinical Microbiology and Infectious Diseases |
| سنة النشر: | 2020 |
| المجموعة: | Université Toulouse III - Paul Sabatier: HAL-UPS |
| مصطلحات موضوعية: | Loop-mediated isothermal amplification, Malaria, QBC, RDT, Rapid diagnostic test, Thick smear, Thin smear, Alethia, Diagnosis, LAMP, MESH: Africa, MESH: Communicable Diseases, Imported / diagnosis, MESH: Molecular Diagnostic Techniques / standards, MESH: Nucleic Acid Amplification Techniques / methods, MESH: Nucleic Acid Amplification Techniques / standards, MESH: Plasmodium, MESH: Prospective Studies, MESH: Real-Time Polymerase Chain Reaction / standards, MESH: Sensitivity and Specificity, MESH: Temperature, Imported / parasitology, MESH: France, MESH: Humans, MESH: Malaria / diagnosis, MESH: Malaria / parasitology, MESH: Microscopy / methods, MESH: Microscopy / standards, MESH: Molecular Diagnostic Techniques / methods, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Malaria is one of most common tropical diseases encountered in travellers and migrants. It requires an urgent and reliable diagnosis considering its potential severity. In this study, performance of five diagnostic assays were evaluated in a nonendemic region and compared prospectively to quantitative PCR (qPCR).Methods: A prospective study was conducted at Toulouse Hospital from August 2017 to January 2018 and included all patients with initial Plasmodium screening. Thin and thick blood smears (TnS, TkS), quantitative buffy coat (QBC), rapid diagnostic tests (RDTs) and commercial loop-mediated isothermal amplification (LAMP) were independently performed on each blood sample and compared to our qPCR reference standard.Results: The study encompassed 331 patients, mainly returning from Africa. qPCR detected 73 Plasmodium-positive samples (including 58 falciparum). Individually, LAMP had a 97.3% (71/73) sensitivity, far ahead of TnS (84.9%, 62/73), TkS (86.3%, 63/73), QBC (86.3%, 63/73) and RDT (86.3%, 63/73). RDT demonstrated a high sensitivity for falciparum (98.3%, 57/58) but missed all ovale, malariae and knowlesi infections. Specificity was excellent for all techniques (99.6-100%). The most sensitive diagnosis strategies were TnS + RDT (95.9%, 70/73), TnS + LAMP (97.3%, 71/73) and TnS + RDT + LAMP (100%, 73/73), about 10% higher than strategies using exclusively microscopy, TkS + TnS (87.7%, 64/73) or QBC + TnS (87.7%, 64/73). TnS remains necessary for Plasmodium species identification and quantification. Adding sequentially TnS only on LAMP-positive samples did not decrease TnS + LAMP strategy sensitivity.Conclusions: In nonendemic countries, the currently recommended microscopy-based strategies seem unsatisfactory for malaria diagnosis considering RDT and LAMP performance, two rapid and sensitive assays that require limited training |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/31158521; hal-03488977; https://hal.science/hal-03488977Test; https://hal.science/hal-03488977/documentTest; https://hal.science/hal-03488977/file/S1198743X19302253.pdfTest; PII: S1198-743X(19)30225-3; PUBMED: 31158521 |
| DOI: | 10.1016/j.cmi.2019.05.010 |
| الإتاحة: | https://doi.org/10.1016/j.cmi.2019.05.010Test https://hal.science/hal-03488977Test https://hal.science/hal-03488977/documentTest https://hal.science/hal-03488977/file/S1198743X19302253.pdfTest |
| حقوق: | http://creativecommons.org/licenses/by-ncTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.5F2F86D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.cmi.2019.05.010 |
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