التفاصيل البيبلوغرافية
| العنوان: |
Effects of clopidogrel bisulfate on B16-F10 cells and tumor development in a murine model of melanoma. |
| المؤلفون: |
Jantsch, Matheus Henrique, Doleski, Pedro Henrique, Viana, Altevir Rossato, da Silva, Jean Lucas Gutknecht, Passos, Daniela Ferreira, Cabral, Fernanda Licker, Manzoni, Alessandra Guedes, Ebone, Renan da Silva, Soares, Ana Bárbara Uchoa, de Andrade, Cínthia Melazzo, Schetinger, Maria Rosa Chitolina, Leal, Daniela Bitencourt Rosa |
| المصدر: |
Biochemistry & Cell Biology; Oct2023, Vol. 101 Issue 5, p443-455, 13p |
| مصطلحات موضوعية: |
CLOPIDOGREL, ROOT-tubercles, MELANOMA, ADENOSINE diphosphate, BLOOD platelet activation, LACTATE dehydrogenase, BLOOD platelet aggregation, CORD blood |
| مستخلص: |
Metastatic melanoma is a very aggressive skin cancer. Platelets are constituents of the tumor microenvironment and, when activated, contribute to cancer progression, especially metastasis and inflammation. P2Y12 is an adenosine diphosphate receptor that triggers platelet activation. Inhibition of P2Y12 by clopidogrel bisulfate (CB) decreases platelet activation, which is also controlled by the extracellular concentration and the metabolism of purines by purinergic enzymes. We evaluated the effects of CB on the viability and proliferation of cultured B16-F10 cells. We also used a metastatic melanoma model with C57BL-6 mice to evaluate cancer development and purine metabolism modulation in platelets. B16-F10 cells were administered intraperitoneally to the mice. Two days later, the animals underwent a 12-day treatment with CB (30 mg/kg by gavage). We have found that CB reduced cell viability and proliferation in B16-F10 culture in 72 h at concentrations above 30 µm. In vivo, CB decreased tumor nodule counts and lactate dehydrogenase levels and increased platelet purine metabolism. Our results showed that CB has significant effects on melanoma progression. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
