التفاصيل البيبلوغرافية
| العنوان: |
A fast and sensitive alternative for β-galactosidase detection in mouse embryos. |
| المؤلفون: |
Sundararajan, Sakthi, Wakamiya, Maki, Behringer, Richard R., Rivera-Pérez, Jaime A. |
| المصدر: |
Development (09501991); 12/1/2012, Vol. 139 Issue 23, p4484-4490, 7p |
| مصطلحات موضوعية: |
GALACTOSIDASES, EMBRYOS, BACTERIAL genes, TRANSGENIC mice, IRON ions, INDOLE compounds, LABORATORY mice |
| مستخلص: |
The bacterial lacZ gene is widely used as a reporter in a myriad of mouse transgenic experiments. β-Galactosidase, encoded by lacZ, is usually detected using X-gal in combination with ferric and ferrous ions. This assay produces a blue indole precipitate that is easy to detect visually. Here, we show that Salmon-gal in combination with tetrazolium salts provides a more sensitive and faster staining reaction than the traditional β-galactosidase assay in mouse embryos. Using a combination of Salmon-gal and tetranitroblue tetrazolium, we were able to visualize the activity of β-galactosidase in embryos at stages when the customary X-gal reaction failed to detect staining. Our studies provide an enhanced alternative for β-galactosidase detection in expression and cell fate studies that use lacZ-based transgenic mouse lines. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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