المؤلفون: Bosteels, Cedric, Scott, Charlotte

المصدر: MOLECULAR IMMUNOLOGY ; ISSN: 0161-5890

مصطلحات موضوعية: Biology and Life Sciences, Medicine and Health Sciences, Immunology, Molecular Biology, Dendritic cells, Transcription Factors, DC fate, Irf4, Irf8, Zeb1, Zeb2, Notch, Tbet, Klf4, MHC CLASS-II, DENDRITIC-CELL-DEVELOPMENT, LYMPHOTOXIN-BETA-RECEPTOR, T-CELLS, SIGNALING CONTROLS, FACTOR E2-2, IFN-GAMMA, DIFFERENTIATION, EXPRESSION

الوصف: Dendritic cells function in the immune system to instruct adaptive immune cells to respond accordingly to different threats. While conventional dendritic cells can be subdivided into two main subtypes, termed cDC1s and cDC2s, it is clear that further heterogeneity exists within these subtypes, particularly for cDC2s. Understanding the signals involved in specifying each of these lineages and subtypes thereof is crucial to (i) enable us to determine their specific functions and (ii) put us in a position to be able to target these cells to promote or prevent a specific function in any given disease setting. Although we still have much to learn regarding the specification of these cells, here we review the most recent advances in our understanding of this and highlight some of the next questions for the future.

وصف الملف: application/pdf

العلاقة: https://biblio.ugent.be/publication/8662391Test; http://hdl.handle.net/1854/LU-8662391Test; http://dx.doi.org/10.1016/j.molimm.2020.02.021Test; https://biblio.ugent.be/publication/8662391/file/8662392Test

الإتاحة: https://doi.org/10.1016/j.molimm.2020.02.021Test
https://biblio.ugent.be/publication/8662391Test
http://hdl.handle.net/1854/LU-8662391Test
https://biblio.ugent.be/publication/8662391/file/8662392Test

المؤلفون: Bosteels, Cedric, Neyt, Katrijn, Vanheerswynghels, Manon, van Helden, Mary J., Sichien, Dorine, Debeuf, Nincy, De Prijck, Sofie, Bosteels, Victor, Vandamme, Niels, Martens, Liesbet, Saeys, Yvan, Louagie, Els, Lesage, Manon, Williams, David L., Tang, Shiau-Choot, Mayer, Johannes U., Ronchese, Franca, Scott, Charlotte L., Hammad, Hamida, Guilliams, Martin, Lambrecht, Bart N.

المصدر: reponame:Expeditio Repositorio Institucional UJTL ; instname:Universidad de Bogotá Jorge Tadeo Lozano

مصطلحات موضوعية: dendritic cell, monocyte, transcription factor, IRF8, virus, CD64, inf-cDC2, inflammation, type 1 interferon, Fc receptor, convalescent serum, Síndrome respiratorio agudo grave, COVID-19, SARS-CoV-2, Coronavirus

الوصف: The phenotypic and functional dichotomy between IRF8+ type 1 and IRF4+ type 2 conventional dendritic cells (cDC1s and cDC2s, respectively) is well accepted; it is unknown how robust this dichotomy is under inflammatory conditions, when additionally monocyte-derived cells (MCs) become competent antigen-presenting cells (APCs). Using single-cell technologies in models of respiratory viral infection, we found that lung cDC2s acquired expression of the Fc receptor CD64 shared with MCs and of IRF8 shared with cDC1s. These inflammatory cDC2s (inf-cDC2s) were superior in inducing CD4+ T helper (Th) cell polarization while simultaneously presenting antigen to CD8+ T cells. When carefully separated from inf-cDC2s, MCs lacked APC function. Inf-cDC2s matured in response to cell-intrinsic Toll-like receptor and type 1 interferon receptor signaling, upregulated an IRF8-dependent maturation module, and acquired antigens via convalescent serum and Fc receptors. Because hybrid inf-cDC2s are easily confused with monocyte-derived cells, their existence could explain why APC functions have been attributed to MCs.

وصف الملف: 28 páginas; application/pdf

العلاقة: https://www.sciencedirect.com/science/article/pii/S1074761320301631?dgcid=rss_sd_all#kwrds0010Test; http://hdl.handle.net/20.500.12010/10969Test; https://doi.org/10.1016/j.immuni.2020.04.005Test

الإتاحة: https://doi.org/20.500.12010/10969Test
https://doi.org/10.1016/j.immuni.2020.04.005Test
https://hdl.handle.net/20.500.12010/10969Test
https://www.sciencedirect.com/science/article/pii/S1074761320301631?dgcid=rss_sd_all#kwrds0010Test

المؤلفون: Bosteels, Cedric, Neyt, Katrijn, Vanheerswynghels, Manon, van Helden, Mary J., Sichien, Dorine, Debeuf, Nincy, De Prijck, Sofie, Bosteels, Victor, Vandamme, Niels, Martens, Liesbet, Saeys, Yvan, Louagie, Els, Lesage, Manon, Williams, David L., Tang, Shiau Choot, Mayer, Johannes U., Ronchese, Franca, Scott, Charlotte L., Hammad, Hamida, Guilliams, Martin, Lambrecht, Bart N.

المصدر: ETSU Faculty Works.

مصطلحات موضوعية: CD64, convalescent serum, dendritic cell, Fc receptor, inf-cDC2, inflammation, IRF8, monocyte, transcription factor, type 1 interferon, virus, Surgery

الوصف: The dichotomy between type 1 and 2 conventional DCs under steady-state conditions is well defined. Bosteels et al. demonstrate that, upon inflammation, cDC2s acquire a hybrid inf-cDC2 phenotype, sharing phenotype, gene expression, and function with cDC1s and monocyte-derived cells, to optimally boost CD4 and CD8 immunity via Fc receptors.

الإتاحة: https://dc.etsu.edu/etsu-works/9152Test

المؤلفون: Sichien, Dorine, Scott, Charlotte L., Martens, Liesbet, Vanderkerken, Matthias, Van Gassen, Sofie, Plantinga, Maud, Joeris, Thorsten, De Prijck, Sofie, Vanhoutte, Leen, Vanheerswynghels, Manon, Van Isterdael, Gert, Toussaint, Wendy, Madeira, Filipe Branco, Vergote, Karl, Agace, William W., Clausen, Björn E., Hammad, Hamida, Dalod, Marc, Saeys, Yvan, Lambrecht, Bart N., Guilliams, Martin

المصدر: Immunity. 45(3):626-640

مصطلحات موضوعية: dendritic cell, development, IRF4, IRF8, lineage, monocyte, plasmacytoid dendritic cell, terminal selector, transcription factor, Medicin och hälsovetenskap, Medicinska och farmaceutiska grundvetenskaper, Immunologi inom det medicinska området, Medical and Health Sciences, Basic Medicine, Immunology in the medical area

الوصف: Interferon regulatory factor-8 (IRF8) has been proposed to be essential for development of monocytes, plasmacytoid dendritic cells (pDCs) and type 1 conventional dendritic cells (cDC1s) and remains highly expressed in differentiated DCs. Transcription factors that are required to maintain the identity of terminally differentiated cells are designated “terminal selectors.” Using BM chimeras, conditional Irf8fl/fl mice and various promotors to target Cre recombinase to different stages of monocyte and DC development, we have identified IRF8 as a terminal selector of the cDC1 lineage controlling survival. In monocytes, IRF8 was necessary during early but not late development. Complete or late deletion of IRF8 had no effect on pDC development or survival but altered their phenotype and gene-expression profile leading to increased T cell stimulatory function but decreased type 1 interferon production. Thus, IRF8 differentially controls the survival and function of terminally differentiated monocytes, cDC1s, and pDCs.

الوصول الحر: https://lup.lub.lu.se/record/31fecae9-1772-4d59-8221-aba1bcc21486Test
http://dx.doi.org/10.1016/j.immuni.2016.08.013Test

المؤلفون: Van Hove, Hannah, Martens, Liesbet, Scheyltjens, Isabelle, De Vlaminck, Karen, Antunes, Ana Rita Pombo, De Prijck, Sofie, Vandamme, Niels, De Schepper, Sebastiaan, Van Isterdael, Gert, Scott, Charlotte, Aerts, Jeroen, Berx, Geert, Boeckxstaene, Guy E., Vandenbroucke, Roosmarijn, Vereecke, Lars, Moechars, Diederik, Guilliams, Martin, Van Ginderachter, Jo A., Saeys, Yvan, Movahedi, Kiavash

المصدر: NATURE NEUROSCIENCE ; ISSN: 1097-6256 ; ISSN: 1546-1726

مصطلحات موضوعية: Biology and Life Sciences, Medicine and Health Sciences, CENTRAL-NERVOUS-SYSTEM, GENE-EXPRESSION, DENDRITIC CELLS, MICROGLIA, DISEASE, IRF8, ORIGIN, PRECURSORS, CYTOSCAPE, MONOCYTES

الوصف: While the roles of parenchymal microglia in brain homeostasis and disease are fairly clear, other brain-resident myeloid cells remain less well understood. By dissecting border regions and combining single-cell RNA-sequencing with high-dimensional cytometry, bulk RNA-sequencing, fate-mapping and microscopy, we reveal the diversity of non-parenchymal brain macrophages. Border-associated macrophages (BAMs) residing in the dura mater, subdural meninges and choroid plexus consisted of distinct subsets with tissue-specific transcriptional signatures, and their cellular composition changed during postnatal development. BAMs exhibited a mixed ontogeny, and subsets displayed distinct self-renewal capacity following depletion and repopulation. Single-cell and fate-mapping analysis both suggested that there is a unique microglial subset residing on the apical surface of the choroid plexus epithelium. Finally, gene network analysis and conditional deletion revealed IRF8 as a master regulator that drives the maturation and diversity of brain macrophages. Our results provide a framework for understanding host-macrophage interactions in both the healthy and diseased brain.

وصف الملف: application/pdf

العلاقة: https://biblio.ugent.be/publication/8619539Test; http://hdl.handle.net/1854/LU-8619539Test; http://doi.org/10.1038/s41593-019-0393-4Test; https://biblio.ugent.be/publication/8619539/file/8619541Test

الإتاحة: https://doi.org/10.1038/s41593-019-0393-4Test
https://biblio.ugent.be/publication/8619539Test
http://hdl.handle.net/1854/LU-8619539Test
https://biblio.ugent.be/publication/8619539/file/8619541Test

المؤلفون: Scott, Charlotte, Soen, Bieke, Martens, Liesbet, Skrypek, Nicolas, Saelens, Wouter, Taminau, Joachim, Blancke, Gillian, Van Isterdael, Gert, Huylebroeck, Danny, Haigh, Jody, Saeys, Yvan, Guilliams, Martin, Lambrecht, Bart, Berx, Geert

المساهمون: Cell biology, Pulmonary Medicine

المصدر: The Journal of Experimental Medicine
Journal of Experimental Medicine, 213(6), 897-911. Rockefeller University Press
JOURNAL OF EXPERIMENTAL MEDICINE

مصطلحات موضوعية: 0301 basic medicine, BONE-MARROW, Plasma Cells, Immunology, FACTOR-BINDING SITES, CD11c, macromolecular substances, Biology, Mice, 03 medical and health sciences, 0302 clinical medicine, TERMINAL DIFFERENTIATION, Conditional gene knockout, Medicine and Health Sciences, Animals, Immunology and Allergy, Psychological repression, Transcription factor, IN-VIVO, Research Articles, SIGNALING CONTROLS, Inhibitor of Differentiation Protein 2, Zinc Finger E-box Binding Homeobox 2, Homeodomain Proteins, Mice, Knockout, Zinc finger, urogenital system, Brief Definitive Report, hemic and immune systems, INTESTINAL LAMINA PROPRIA, Dendritic Cells, CLONOGENIC PROGENITOR, Up-Regulation, 3. Good health, Repressor Proteins, DENDRITIC CELL-DEVELOPMENT, 030104 developmental biology, SUBSET DEVELOPMENT, FACTOR E2-2, Cancer research, IRF8, Chromatin immunoprecipitation, 030215 immunology

الوصف: Lambrecht et al. show that the transcription factor Zeb2 regulates commitment toward both the pDC and cDC2 lineages by repressing Id2.
Plasmacytoid dendritic cells (DCs [pDCs]) develop from pre-pDCs, whereas two lineages of conventional DCs (cDCs; cDC1s and cDC2s) develop from lineage-committed pre-cDCs. Several transcription factors (TFs) have been implicated in regulating the development of pDCs (E2-2 and Id2) and cDC1s (Irf8, Id2, and Batf3); however, those required for the early commitment of pre-cDCs toward the cDC2 lineage are unknown. Here, we identify the TF zinc finger E box–binding homeobox 2 (Zeb2) to play a crucial role in regulating DC development. Zeb2 was expressed from the pre-pDC and pre-cDC stage onward and highly expressed in mature pDCs and cDC2s. Mice conditionally lacking Zeb2 in CD11c+ cells had a cell-intrinsic reduction in pDCs and cDC2s, coupled with an increase in cDC1s. Conversely, mice in which CD11c+ cells overexpressed Zeb2 displayed a reduction in cDC1s. This was accompanied by altered expression of Id2, which was up-regulated in cDC2s and pDCs from conditional knockout mice. Zeb2 chromatin immunoprecipitation analysis revealed Id2 to be a direct target of Zeb2. Thus, we conclude that Zeb2 regulates commitment to both the cDC2 and pDC lineages through repression of Id2.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::052dd30797bc26813b606565b5c183ebTest
https://doi.org/10.1084/jem.20151715Test

المؤلفون: Van der Borght, Katrien, Scott, Charlotte L., Nindl, Veronika, Bouché, Ann, Martens, Liesbet, Sichien, Dorine, Van Moorleghem, Justine, Vanheerswynghels, Manon, De Prijck, Sofie, Saeys, Yvan, Ludewig, Burkhard, Gillebert, Thierry, Guilliams, Martin, Carmeliet, Peter, Lambrecht, Bart N.

المصدر: Cell Reports; Mar2017, Vol. 18 Issue 12, p3005-3017, 13p

مستخلص: Summary Peripheral tolerance is crucial for avoiding activation of self-reactive T cells to tissue-restricted antigens . Sterile tissue injury can break peripheral tolerance, but it is unclear how autoreactive T cells get activated in response to self. An example of a sterile injury is myocardial infarction (MI). We hypothesized that tissue necrosis is an activator of dendritic cells (DCs), which control tolerance to self-antigens. DC subsets of a murine healthy heart consisted of IRF8-dependent conventional (c)DC1, IRF4-dependent cDC2, and monocyte-derived DCs. In steady state, cardiac self-antigen α-myosin was presented in the heart-draining mediastinal lymph node (mLN) by cDC1s, driving the proliferation of antigen-specific CD4 + TCR-M T cells and their differentiation into regulatory cells (Tregs). Following MI, all DC subsets infiltrated the heart, whereas only cDCs migrated to the mLN. Here, cDC2s induced TCR-M proliferation and differentiation into interleukin-(IL)-17/interferon-(IFN)γ-producing effector cells. Thus, cardiac-specific autoreactive T cells get activated by mature DCs following myocardial infarction. [ABSTRACT FROM AUTHOR]

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