Xenopus oocytes as a heterologous expression system for studying ion channels with the patch-clamp technique
| العنوان: | Xenopus oocytes as a heterologous expression system for studying ion channels with the patch-clamp technique |
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| المؤلفون: | Kenju Shimomura, Paolo Tammaro, Peter Proks |
| المصدر: | Methods in Molecular Biology ISBN: 9781934115657 |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | biology, Chemistry, urogenital system, Xenopus, Heterologous, Oocyte, biology.organism_classification, Cell biology, Electrophysiology, medicine.anatomical_structure, medicine, Patch clamp, Heterologous expression, Large size, Ion channel |
| الوصف: | Oocytes from the Xenopus laevis represent one of the most widely used expression systems for functional characterization of ion channels. Their large size facilitates both injection of heterologous cRNA and subsequent electrophysiological recordings of ion channel currents. Furthermore, Xenopus oocytes translate cRNA very efficiently, resulting in the generation of a large number of ion channels in the plasma membrane. In this chapter, we outline methods for oocyte preparation and maintenance and describe procedures for patch-clamping of oocytes, with a special focus on the macropatch technique. We discuss some common problems associated with patch-clamping of oocytes and their use as an expression system for ion channels. |
| اللغة: | English |
| ردمك: | 978-1-934115-65-7 |
| تدمد: | 1940-6029 1064-3745 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::37422244f1a468106085a69dcd856e1eTest http://ora.ox.ac.uk/objects/uuid:06a2ee56-5b26-4eb4-8511-013af0b80133Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....37422244f1a468106085a69dcd856e1e |
| قاعدة البيانات: | OpenAIRE |
| ردمك: | 9781934115657 |
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| تدمد: | 19406029 10643745 |