التفاصيل البيبلوغرافية
| العنوان: |
Intra-articular Recombinant Human Proteoglycan 4 Mitigates Cartilage Damage After Destabilization of the Medial Meniscus in the Yucatan Minipig. |
| المؤلفون: |
Waller, Kimberly A.1,2, Chin, Kaitlyn E.1,2, McAllister, Scott1,2, Fleming, Braden C.1,2,3, Zhang, Ling X.2,4, Jay, Gregory D.2,3,4, Teeple, Erin2,5,6, Badger, Gary J.2,7, Schmidt, Tannin A.2,8 |
| المصدر: |
American Journal of Sports Medicine. Jun2017, Vol. 45 Issue 7, p1512-1521. 10p. |
| مصطلحات موضوعية: |
*INFLAMMATION prevention, *MENISCUS injuries, *ANIMAL experimentation, *ARTICULAR cartilage, *COLLAGEN, *ENZYME-linked immunosorbent assay, *GLYCOPROTEINS, *HISTOLOGICAL techniques, *INTRA-articular injections, *INTERLEUKINS, *MICROSCOPY, *PEPTIDES, *PROBABILITY theory, *RECOMBINANT proteins, *RESEARCH funding, *STAINS & staining (Microscopy), *SWINE, *SYNOVIAL fluid, *STATISTICAL power analysis, *EFFECT sizes (Statistics), *DATA analysis software, *DESCRIPTIVE statistics, *MANN Whitney U Test, *KRUSKAL-Wallis Test, *ONE-way analysis of variance, *IN vivo studies, *THERAPEUTICS |
| مصطلحات جغرافية: |
RHODE Island |
| مستخلص: |
Background: Lubricin, or proteoglycan 4 (PRG4), is a glycoprotein responsible for joint boundary lubrication. PRG4 has been shown previously to be down-regulated after traumatic joint injury such as a meniscal tear. Preliminary evidence suggests that intra-articular injection of PRG4 after injury will reduce cartilage damage in rat models of surgically induced posttraumatic osteoarthritis. Objective: To determine the efficacy of intra-articular injection of full-length recombinant human lubricin (rhPRG4) for reducing cartilage damage after medial meniscal destabilization (DMM) in a preclinical large animal model. Study Design: Controlled laboratory study. Methods: Unilateral DMM was performed in 29 Yucatan minipigs. One week after DMM, animals received 3 weekly intra-articular injections (3 mL per injection): (1) rhPRG4 (1.3 mg/mL; n = 10); (2) rhPRG4+hyaluronan (1.3 mg/mL rhPRG4 and 3 mg/mL hyaluronan [~950 kDA]; n = 10); and (3) phosphate-buffered saline (PBS; n = 9). Hindlimbs were harvested 26 weeks after surgery. Cartilage integrity was evaluated by use of macroscopic (India ink) and microscopic (safranin O–fast green and hematoxylin and eosin) scoring systems. Secondary outcomes evaluated via enzyme-linked immunosorbent assay (ELISA) included PRG4 levels in synovial fluid, carboxy-terminal telepeptide of type II collagen (CTX-II) concentrations in urine and serum, and interleukin 1β (IL-1β) levels in synovial fluid and serum. Results: The rhPRG4 group had significantly less macroscopic cartilage damage in the medial tibial plateau compared with the PBS group (P = .002). No difference was found between the rhPRG4+hyaluronan and PBS groups (P = .23). However, no differences in microscopic damage scores were observed between the 3 groups (P = .70). PRG4 production was elevated in the rhPRG4 group synovial fluid compared with the PBS group (P = .033). The rhPRG4 group presented significantly lower urinary CTX-II levels, but not serum levels, when compared with the PBS (P = .013) and rhPRG4+hyaluronan (P = .011) groups. In serum and synovial fluid, both rhPRG4 (P = .006; P = .017) and rhPRG4+hyaluronan groups (P = .009; P = .03) presented decreased IL-1β levels. Conclusion: All groups exhibited significant cartilage degeneration after DMM surgery. However, animals treated with rhPRG4 had the least amount of cartilage damage and less inflammation, providing evidence that intra-articular injections of rhPRG4 may slow the progression of posttraumatic osteoarthritis. Clinical Relevance: Patients with meniscal trauma are at high risk for posttraumatic osteoarthritis. This study demonstrates that an intra-articular injection regimen of rhPRG4 may attenuate cartilage damage after meniscal injury. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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