التفاصيل البيبلوغرافية
| العنوان: |
The role of intracisternal A-type particles in the evolution of factor-independent murine hematopoietic cell lines. |
| المؤلفون: |
Pogue-Geile, K L, Gott, J A, Greenberger, J S |
| المصدر: |
Leukemia (08876924). Jan98, Vol. 12 Issue 1, p4-12. 9p. |
| مصطلحات موضوعية: |
*HEMATOPOIESIS, *CELL lines, *ANIMAL experimentation, *BONE marrow, *CELL culture, *CELLS, *COMPARATIVE studies, *CONNECTIVE tissue cells, *CULTURE media (Biology), *DNA probes, *DOCUMENTATION, *GENES, *GRANULOCYTE-macrophage colony-stimulating factor, *HEMATOPOIETIC stem cells, *INTERLEUKIN-3, *RESEARCH methodology, *MEDICAL cooperation, *MICE, *GENETIC mutation, *NUCLEOTIDES, *POLYMERASE chain reaction, *RESEARCH, *TISSUE culture, *EVALUATION research |
| مستخلص: |
Cocultivation of a clonal factor-dependent hematopoietic cell line (FDC-P1JL26) with an irradiated bone marrow stromal cell line (D2XRII) significantly increased the frequency of isolation of factor-independent subclones. Eight out of nine factor-independent subclonal lines showed expression of IL-3, GM-CSF or both cytokine mRNAs by reverse-transcription polymerase chain reaction (RT-PCR) and seven of these expressed biologically active GM-CSF or IL-3. In three cell lines that synthesized biologically active IL-3 (FIJ1, FIJ4D and FIJ10D) insertion of an IAP sequence into the IL-3 gene was detected by PCR analysis and the insertions were confirmed by DNA sequence analysis of PCR or RT-PCR fragments. In the four cell lines in which no IL-3 expression was detected no IAP insertions were detected. Rearrangements of the GM-CSF gene were detected in three factor-independent cell lines and an insertion of an IAP into the GM-CSF gene was confirmed by DNA sequence analysis of PCR fragments. In contrast to results with IL-3, insertion of an IAP into the GM-CSF gene did not correlate with GM-CSF expression. In one cell line that contained an IAP insertion into the GM-CSF gene, no GM-CSF was detected by biological assay nor by RT-PCR. Retrotransposition of IAPs may be responsible for the emergence of factor-independent cells in our cocultivation system and other IAP insertions may prove to be responsible for the factor-independent phenotype seen in the non-autocrine factor-independent cell line, FI7CL2. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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