التفاصيل البيبلوغرافية
| العنوان: |
IL-18 Cytokine Levels Modulate Innate Immune Responses and Cryptosporidiosis in Mice. |
| المؤلفون: |
Bedi, Brahmchetna1,2, McNair, Nina N.2, Förster, Irmgard3, Mead, Jan R.1,2 |
| المصدر: |
Journal of Eukaryotic Microbiology. Jan2015, Vol. 62 Issue 1, p44-50. 7p. |
| مصطلحات موضوعية: |
*CRYPTOSPORIDIUM parvum, *INTERLEUKIN-18, *SEVERE combined immunodeficiency, *PEPTIDE antibiotics, *OPPORTUNISTIC infections, *IMMUNOLOGY |
| مستخلص: |
IL-18 is known to play a key role limiting Cryptosporidium parvum infection. In this study, we show that IL-18 depletion in SCID mice significantly exacerbates C. parvum infection, whereas, treatment with recombinant IL-18 (r IL-18), significantly decreases the parasite load, as compared to controls. Increases in serum IFN-γ levels as well as the up-regulation of the antimicrobial peptides, cathelicidin antimicrobial peptide and beta defensin 3 ( Defb3) were observed in the intestinal mucosa of mice treated with r IL-18. In addition, C. parvum infection significantly increased m RNA expression levels (> 50 fold) of the alpha defensins, Defa3 and 5, respectively. Interestingly, we also found a decrease in m RNA expression of IL-33 (a recently identified cytokine in the same family as IL-18) in the small intestinal tissue from mice treated with r IL-18. In comparison, the respective genes were induced by IL-18 depletion. Our findings suggest that IL-18 can mediate its protective effects via different routes such as IFN-γ induction or by directly stimulating intestinal epithelial cells to increase antimicrobial activity. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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