دورية أكاديمية
Gastrin regulates the heparin-binding epidermal-like growth factor promoter via a PKC/EGFR-dependent mechanism
| العنوان: | Gastrin regulates the heparin-binding epidermal-like growth factor promoter via a PKC/EGFR-dependent mechanism |
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| المؤلفون: | Sinclair, Natalie F., Ai, Wandong, Raychowdhury, Raktima, Bi, Meixia, Wang, Timothy C., Koh, Theodore J., McLaughlin, John T. |
| المساهمون: | University of Massachusetts Memorial Medical Center, Division of Digestive Diseases and Nutrition |
| المصدر: | American journal of physiology. Gastrointestinal and liver physiology ; 286 ; 6 ; G992-9 |
| سنة النشر: | 2022 |
| المجموعة: | University of Massachusetts, Medical School: eScholarship@UMMS |
| مصطلحات موضوعية: | Animals, Cell Line, Tumor, Cell Nucleus, Epidermal Growth Factor, Gastrins, Humans, Intercellular Signaling Peptides and Proteins, Mice, Promoter Regions (Genetics), Protein Kinase C, Receptor, Response Elements, Signal Transduction, Sp1 Transcription Factor, Stereoisomerism, Transcription Factors, Ultraviolet Rays, Zinc, Life Sciences, Medicine and Health Sciences |
| الوصف: | Gastrin is a known growth/differentiation factor for the gastric mucosa. Its effects are likely mediated by the induction of heparin-binding epidermal-like growth factor (HB-EGF), a member of the EGF family of growth factors that is expressed by gastric parietal cells. In this study, we investigated the regulation of the HB-EGF promoter by gastrin in a human gastric cancer cell line. Serial human HB-EGF promoter-luciferase reporter deletion constructs and heterologous promoter constructs were transfected into AGS-E cells and stimulated with gastrin (10(-7) M) with or without various signal transduction inhibitors. EMSA were also performed. Gastrin stimulation resulted in a fivefold increase in HB-EGF-luciferase activity. The cis-acting element mediating gastrin responsiveness was mapped to the -69 to -58 region of the HB-EGF promoter. Gastrin stimulation was PKC dependent and at least partially mediated by activation of the EGF receptor. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | Link to article in PubMed; http://dx.doi.org/10.1152/ajpgi.00206.2002Test; Am J Physiol Gastrointest Liver Physiol. 2004 Jun;286(6):G992-9. Link to article on publisher's site; 0193-1857 (Print); http://hdl.handle.net/20.500.14038/38321Test; https://escholarship.umassmed.edu/oapubs/119Test; 417327; oapubs/119 |
| DOI: | 10.1152/ajpgi.00206.2002 |
| الإتاحة: | https://doi.org/10.1152/ajpgi.00206.2002Test https://doi.org/20.500.14038/38321Test https://hdl.handle.net/20.500.14038/38321Test https://escholarship.umassmed.edu/oapubs/119Test |
| رقم الانضمام: | edsbas.53A6780D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1152/ajpgi.00206.2002 |
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