التفاصيل البيبلوغرافية
| العنوان: |
Investigation of carbapenem-resistant Enterobacterales isolates at a tertiary laboratory in Pretoria, South Africa. |
| المؤلفون: |
Ramashia, Mbudzeni, Phofa, Thabiso D., Nkawane, Granny M., Nogbou, Noel-David, Bolukaoto, John Y., Nchabeleng, Maphoshane, Musyoki, Andrew M. |
| المصدر: |
Acta Microbiologica et Immunologica Hungarica; Dec2023, Vol. 70 Issue 4, p295-303, 9p |
| مصطلحات موضوعية: |
RESOURCE-limited settings, ENTEROBACTER cloacae, KLEBSIELLA pneumoniae, CARBAPENEMASE, GENOTYPES, INTENSIVE care units |
| مصطلحات جغرافية: |
PRETORIA (South Africa), SOUTH Africa |
| مستخلص: |
This study aimed to investigate phenotypic and genotypic characteristics of carbapenem-resistant Enterobacterales isolates (CRE) at a tertiary laboratory in South Africa. A total of 99 CRE isolates were collected between 2019 and 2021. Carbapenemase production was tested using modified carbapenem inhibitory method. Colistin susceptibility testing was performed using the ComASP™ Colistin broth microdilution method. Conventional PCR assays were conducted for detection of mcr-1 gene and common carbapenemase genes (bla VIM , bla NDM , bla IMP , bla KPC , bla OXA-23 , bla OXA-51 and bla OXA-48). Rep-PCR assay was conducted to determine the genetic relatedness of the study isolates. Majority of the isolates were Klebsiella pneumoniae (83%). Carbapenem resistant K. pneumoniae cluster was observed from ICU and surgical ward samples. Colistin resistance was observed in 13% (12/93) of the isolates namely, in 11 K. pneumoniae and one Enterobacter cloacae. The bla OXA-48 (65%) was the most prevalent gene detected followed by bla NDM (25%) and bla VIM (22%). Several K. pneumoniae isolates concomitantly carried multiple carbapenemase genes with one isolate carry up to 5 five genes bla VIM, bla NDM, bla OXA-48, bla OXA-23 and bla OXA-51. The mcr-1 gene was not detected in the isolates. Rep-PCR assay showed that most isolates matched cluster A (50%). The high prevalence of bla OXA-48, bla NDM and emerging colistin resistant isolates is of concern for patient management at this institution and needs close monitoring. Rep-PCR is a valuable tool in establishing infection clusters in resource-limited settings. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
