التفاصيل البيبلوغرافية
| العنوان: |
Hierarchical integration of DNA nanostructures and NanoGold onto a microchip facilitates covalent chemistry-mediated purification of circulating tumor cells in head and neck squamous cell carcinoma. |
| المؤلفون: |
Sun, Na, Zhang, Ceng, Wang, Jing, Yue, Xinmin, Kim, Hyo Yong, Zhang, Ryan Y., Liu, Hongtao, Widjaja, Josephine, Tang, Hubert, Zhang, Tiffany X., Ye, Jinglei, Qian, Audrey, Liu, Chensong, Wu, Alex, Wang, Katharina, Johanis, Michael, Yang, Peng, Liu, Honggang, Meng, Meng, Liang, Li |
| المصدر: |
Nano Today; Apr2023, Vol. 49, pN.PAG-N.PAG, 1p |
| مصطلحات موضوعية: |
SQUAMOUS cell carcinoma, DNA nanotechnology, ONCOGENES, HUMAN papillomavirus, HEAD tumors, INTEGRATED circuits, DNA |
| مستخلص: |
It is well-established that the combined use of nanostructured substrates and immunoaffinity agents can enhance the cell-capture performance of the substrates, thus offering a practical solution to effectively capture circulating tumor cells (CTCs) in peripheral blood. Developing along this strategy, this study first demonstrated a top-down approach for the fabrication of tetrahedral DNA nanostructure (TDN)-NanoGold substrates through the hierarchical integration of three functional constituents at various length-scales: a macroscale glass slide, sub-microscale self-organized NanoGold, and nanoscale self-assembled TDN. The TDN-NanoGold substrates were then assembled with microfluidic chaotic mixers to give TDN-NanoGold Click Chips. In conjunction with the use of copper (Cu)-catalyzed azide-alkyne cycloaddition (CuAAC)-mediated CTC capture and restriction enzyme-triggered CTC release, TDN-NanoGold Click Chips allow for effective enumeration and purification of CTCs with intact cell morphologies and preserved molecular integrity. To evaluate the clinical utility of TDN-NanoGold Click Chips, we used these devices to isolate and purify CTCs from patients with human papillomavirus (HPV)-positive (+) head and neck squamous cell carcinoma (HNSCC). The purified HPV(+) HNSCC CTCs were then subjected to RT-ddPCR testing, allowing for detection of E6/E7 oncogenes, the characteristic molecular signatures of HPV(+) HNSCC. We found that the resulting HPV(+) HNSCC CTC counts and E6/E7 transcript copy numbers are correlated with the treatment responses in the patients, suggesting the potential clinical utility of TDN-NanoGold Click Chips for noninvasive diagnostic applications of HPV(+) HNSCC. [Display omitted] • Construction of tetrahedral DNA nanostructure-NanoGold substrate for purifying CTCs. • TDN-NanoGold Click Chip combined CuAAC and restriction enzyme for CTC purification. • HPV E6/E7 oncogenes were detected in CTCs purified from HPV(+) HNSCC patients. • TDN-NanoGold Click Chip can be used for noninvasive diagnosis of HPV(+) HNSCC. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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