A Vacuolar Sorting Receptor PV72 on the Membrane of Vesicles that Accumulate Precursors of Seed Storage Proteins (PAC Vesicles)
| العنوان: | A Vacuolar Sorting Receptor PV72 on the Membrane of Vesicles that Accumulate Precursors of Seed Storage Proteins (PAC Vesicles) |
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| المؤلفون: | Ikuko Hara-Nishimura, Mikio Nishimura, Kentaro Tamura, Etsuko Watanabe, Tomoo Shimada, Yasuko Hayashi |
| المصدر: | Plant and Cell Physiology. 43:1086-1095 |
| بيانات النشر: | Oxford University Press (OUP), 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | Physiology, Recombinant Fusion Proteins, Green Fluorescent Proteins, Protein storage vacuole, Arabidopsis, Golgi Apparatus, Receptors, Cytoplasmic and Nuclear, Plant Science, Vacuole, Biology, Transfection, symbols.namesake, food, Storage protein, Integral membrane protein, Plant Proteins, chemistry.chemical_classification, Pumpkin seed, Vesicle, Calcium-Binding Proteins, Biological Transport, Clathrin-Coated Vesicles, Cell Biology, General Medicine, Golgi apparatus, Fusion protein, food.food, Cell biology, Luminescent Proteins, Microscopy, Electron, chemistry, Seeds, Vacuoles, symbols, Signal Transduction |
| الوصف: | A novel vesicle, referred to as a precursor-accumulating (PAC) vesicle, mediates the transport of storage protein precursors to protein storage vacuoles in maturing pumpkin seeds. PV72, a type I integral membrane protein with three repeats of epidermal growth factor, was found on the membrane of the PAC vesicles. PV72 had an ability to bind to pro2S albumin, a storage protein precursor, in a Ca(2+)-dependent manner, via the C-terminal region of pro2S albumin, which was found to function as a vacuolar targeting signal. This implies that PV72 is a vacuolar sorting receptor of the storage protein. PV72 was specifically and transiently accumulated at the middle stage of seed maturation in association with the synthesis of storage proteins. Subcellular fractionation showed that PV72 was also accumulated in the microsomal fraction. A fusion protein consisting of GFP and the transmembrane domain and the cytosolic tail of PV72 was localized in Golgi complex. PV72 in the isolated PAC vesicles had a complex type of oligosaccharide, indicating that PV72 passed though the Golgi complex. These results suggest that PV72 is recycled between PAC vesicles and Golgi complex/post-Golgi compartments. PV72 appears to be responsible for recruiting pro2S albumin molecules from the Golgi complex to the PAC vesicles. |
| تدمد: | 1471-9053 0032-0781 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb69d21a23f1126350b70c508739997cTest https://doi.org/10.1093/pcp/pcf152Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....cb69d21a23f1126350b70c508739997c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14719053 00320781 |
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