دورية أكاديمية
Isolation of Functional SARS-CoV-2 Antigen-Specific T-Cells with Specific Viral Cytotoxic Activity for Adoptive Therapy of COVID-19
| العنوان: | Isolation of Functional SARS-CoV-2 Antigen-Specific T-Cells with Specific Viral Cytotoxic Activity for Adoptive Therapy of COVID-19 |
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| المؤلفون: | García Ríos, Estéfani, Leivas, Alejandra, Mancebo, Francisco J., Sánchez Vega, Laura, Lanzarot, Diego, Aguado García, José María, Martínez López, Joaquín, Paciello, María Liz, Pérez Romero, Pilar |
| بيانات النشر: | MPDI |
| سنة النشر: | 2022 |
| المجموعة: | Universidad Complutense de Madrid (UCM): E-Prints Complutense |
| مصطلحات موضوعية: | SARS-CoV-2, T-lymphocytes, cytotoxicity, M protein, Inmunología, 2412 Inmunología |
| الوصف: | In order to demonstrate the feasibility of preparing clinical-grade SARS-CoV-2-specific T-cells from convalescent donors and the ability of these cells to neutralize the virus in vitro, we used blood collected from two COVID-19 convalescent donors (before and after vaccination) that was stimulated with specific SARS-CoV-2 peptides followed by automated T-cell isolation using the CliniMacs Prodigy medical device. To determine cytotoxic activity, HEK 293T cells were transfected to express the SARS-CoV-2 M protein, mimicking SARS-CoV-2 infection. We were able to quickly and efficiently isolate SARS-CoV-2-specific T lymphocytes from both donors before and after they received the Pfizer-BioNTech vaccine. Althoughbefore vaccination, the final product contained up to 7.42% and 30.19% of IFN-γ+ CD3+ T-cells from donor 1 and donor 2, respectively, we observed an enrichment of the IFN-γ+ CD3+ T-cells after vaccination, reaching 70.47% and 42.59%, respectively. At pre-vaccination, the isolated SARS-CoV-2-specific T-cells exhibited cytotoxic activity that was significantly higher than that of unstimulated controls (donor 2: 15.41%, p-value 3.27 × 10−3). The cytotoxic activity of the isolated SARS-CoV-2-specific T-cells also significantly increased after vaccination (donor 1: 32.71%, p-value 1.44 × 10−5; donor 2: 33.38%, p-value 3.13 × 10−6). In conclusion, we demonstrated that SARS-CoV-2-specific T-cells can quickly and efficiently be stimulated from the blood of convalescent donors using SARS-CoV-2-specific peptides followed by automated isolation. Vaccinated convalescent donors have a higher percentage of SARS-CoV-2-specific T-cells and may be more suitable as donors. Although further studies are needed to assess the clinical utility of the functional isolated SARS-CoV-2-specific T-cells in patients, previous studies using the same stimulation and isolation methods applied to other pathologies support this idea. ; Depto. de Medicina ; Fac. de Medicina ; TRUE ; Ministerio de Ciencia e Innovación (MICINN)/FEDER ; Instituto ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2227-9059 |
| العلاقة: | (REIPI RD16/0016); (COVID-19 Research Call COV20/00181); (F18III/00013); (CD18CIII/00007); REACT-EU; https://doi.org/10.3390/biomedicines10030630Test; https://www.mdpi.com/2227-9059/10/3/630/htmTest; https://hdl.handle.net/20.500.14352/71610Test |
| DOI: | 10.3390/biomedicines10030630 |
| الإتاحة: | https://doi.org/20.500.14352/71610Test https://doi.org/10.3390/biomedicines10030630Test https://hdl.handle.net/20.500.14352/71610Test https://www.mdpi.com/2227-9059/10/3/630/htmTest |
| حقوق: | Atribución 3.0 España ; open access ; https://creativecommons.org/licenses/by/3.0/esTest/ |
| رقم الانضمام: | edsbas.94FC7625 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 22279059 |
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| DOI: | 10.3390/biomedicines10030630 |