دورية أكاديمية
Phenotypic and functional heterogeneity of human intermediate monocytes based on HLA-DR expression
| العنوان: | Phenotypic and functional heterogeneity of human intermediate monocytes based on HLA-DR expression |
|---|---|
| المؤلفون: | Connaughton, Eanna P., Naicker, Serika, Hanley, Shirley A., Slevin, Stephanie M., Eykelenboom, John K., Lowndes, Noel F., O'Brien, Timothy, Ceredig, Rhodri, Griffin, Matthew D., Dennedy, Michael C. |
| المساهمون: | Molecular Medicine Ireland Clinical, Health Services Executive, Health Research Board, Irish Research Council for Science, Engineering and Technology, Science Foundation Ireland, Horizon 2020, European Regional Development Fund |
| بيانات النشر: | Wiley |
| سنة النشر: | 2019 |
| المجموعة: | National University of Ireland (NUI), Galway: ARAN |
| مصطلحات موضوعية: | Adhesion molecule, atherosclerosis, chemokine, lipoproteins, migration obesity, scavenger receptor, CHRONIC KIDNEY-DISEASE, DENDRITIC CELLS, CD14(++)CD16(+) MONOCYTES, ENDOTHELIAL-CELLS, BLOOD MONOCYTES, CROHNS-DISEASE, HUMAN OBESITY, SUBPOPULATIONS, INFLAMMATION |
| الوصف: | Human blood monocytes are subclassified as classical, intermediate and nonclassical. In this study, it was shown that conventionally defined human intermediate monocytes can be divided into two distinct subpopulations with mid- and high-level surface expression of HLA-DR (referred to as DRmid and DRhi intermediate monocytes). These IM subpopulations were phenotypically and functionally characterized in healthy adult blood by flow cytometry, migration assays and lipoprotein uptake assays. Their absolute numbers and proportions were then compared in blood samples from obese and nonobese adults. DRmid and DRhi intermediate monocytes differentially expressed several proteins including CD62L, CD11a, CX3CR1 and CCR2. Overall, the DRmid intermediate monocytes surface profile more closely resembled that of classical monocytes while DRhi intermediate monocytes were more similar to nonclassical. However, in contrast to classical monocytes, DRmid intermediate monocytes migrated weakly to CCL2, had reduced intracellular calcium flux following CCR2 ligation and favored adherence to TNF alpha-activated endothelium over transmigration. In lipid uptake assays, DRmid intermediate monocytes demonstrated greater internalization of oxidized and acetylated low-density lipoprotein than DRhi intermediate monocytes. In obese compared to nonobese adults, proportions and absolute numbers of DRmid, but not DRhi intermediate monocytes, were increased in blood. The results are consistent with phenotypic and functional heterogeneity within the intermediate monocytes subset that may be of specific relevance to lipoprotein scavenging and metabolic health. ; The authors acknowledge the kind contribution of time and samples by study participants, as well as the assistance of the nursing and medical staff at the Centre for Diabetes, Endocrinology and Metabolism (CDEM) at Galway University Hospital. We also acknowledge assistance provided by Dr Paula O'Shea, Consultant Biochemist and staff at the Department of Biochemistry, Galway University ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1440-1711 |
| العلاقة: | Immunol Cell Biol; Connaughton, Eanna P, Naicker, Serika, Hanley, Shirley A, Slevin, Stephanie M, Eykelenboom, John K, Lowndes, Noel F, O'Brien, Timothy, Ceredig, Rhodri, Griffin, Matthew D, Dennedy, Michael C. (2018). Phenotypic and functional heterogeneity of human intermediate monocytes based on HLA-DR expression. Immunology & Cell Biology, 96(7), 742-758. doi:10.1111/imcb.12032; http://hdl.handle.net/10379/15013Test |
| DOI: | 10.1111/imcb.12032 |
| الإتاحة: | https://doi.org/10.1111/imcb.12032Test http://hdl.handle.net/10379/15013Test |
| حقوق: | Attribution-NonCommercial-NoDerivs 3.0 Ireland ; https://creativecommons.org/licenses/by-nc-nd/3.0/ieTest/ |
| رقم الانضمام: | edsbas.CFBD4079 |
| قاعدة البيانات: | BASE |
| تدمد: | 14401711 |
|---|---|
| DOI: | 10.1111/imcb.12032 |