Stabilization of HIF-1α in Human Retinal Endothelial Cells Modulates Expression of miRNAs and Proangiogenic Growth Factors
| العنوان: | Stabilization of HIF-1α in Human Retinal Endothelial Cells Modulates Expression of miRNAs and Proangiogenic Growth Factors |
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| المؤلفون: | Francesca Lazzara, Maria Consiglia Trotta, Chiara Bianca Maria Platania, Michele D’Amico, Francesco Petrillo, Marilena Galdiero, Carlo Gesualdo, Settimio Rossi, Filippo Drago, Claudio Bucolo |
| المساهمون: | Lazzara, Francesca, Trotta, Maria Consiglia, Bianca Maria Platania, Chiara, D'Amico, Michele, Petrillo, Francesco, Galdiero, Marilena, Gesualdo, Carlo, Rossi, Settimio, Drago, Filippo, Bucolo, Claudio |
| المصدر: | Frontiers in Pharmacology, Vol 11 (2020) Frontiers in Pharmacology |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, retina, Angiogenesis, hypoxia-inducible-factor-1α, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Downregulation and upregulation, medicine, Pharmacology (medical), Original Research, Pharmacology, transforming growth factor beta, Retina, biology, vascular endothelial growth factor, lcsh:RM1-950, Retinal, Transforming growth factor beta, Vascular endothelial growth factor, Vascular endothelial growth factor A, diabetic retinopathy, 030104 developmental biology, medicine.anatomical_structure, lcsh:Therapeutics. Pharmacology, chemistry, inflammation, 030220 oncology & carcinogenesis, Cancer research, biology.protein, Signal transduction |
| الوصف: | Retinal hypoxia is one of the causative factors of diabetic retinopathy and is also one of the triggers of VEGF release. We hypothesized that specific dysregulated miRNAs in diabetic retinopathy could be linked to hypoxia-induced damage in human retinal endothelial cells (HRECs). We investigated in HRECs the effects of chemical (CoCl2) hypoxia on the expression of HIF-1α, VEGF, PlGF, and of a focused set of miRNAs. We found that miR-20a-5p, miR-20b-5p, miR-27a-3p, miR-27b-3p, miR-206-3p, miR-381-3p correlated also with expression of TGFβ signaling pathway genes in HRECs, challenged with chemical hypoxic stimuli. In conclusion, our data suggest that retinal angiogenesis would be promoted, at least under HIF-1α activation, by upregulation of PlGF and other factors such as miRNAs, VEGFA, and TGFβ1. |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f20f81eaac250d1e8526f748eaa01a0bTest http://hdl.handle.net/20.500.11769/496465Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f20f81eaac250d1e8526f748eaa01a0b |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |