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1رسالة جامعية
المؤلفون: Medeiros, Gislane Batista de Oliveira
المساهمون: Bezerra, DanieDanielle Soareslle, 7233941180667766, http://lattes.cnpq.br/7233941180667766Test, 4545867903131219, http://lattes.cnpq.br/4545867903131219Test, Fayh, Ana Paula Trussardi, 0049770583345803, http://lattes.cnpq.br/0049770583345803Test, Virgens, Isabel Pinto Amorim das, 1697690465170133, http://lattes.cnpq.br/1697690465170133Test
مصطلحات موضوعية: Ácidos graxos ômega-3, Estado nutricional, Sars-Cov-2, Covid-19, Inflamação, Omega-3 fatty acids, Nutritional status, Inflammation, CNPQ::CIENCIAS DA SAUDE::NUTRICAO
الوصف: INTRODUCTION: Covid-19 is a contagious infection in humans, whose clinical picture can present itself in various ways, from symptoms such as fever, nasal congestion and cough, to severe manifestations such as multiple organ failure and death, related to the exacerbated inflammatory process, characteristic of the disease. Knowing that some nutrients, such as omega-3, have the ability to act positively on immunity and partially inhibit various aspects of inflammation, this study aimed to prepare an integrative review to assess the potential benefits of adequate blood omega-3 status and omega-3 supplementation in patients with covid-19. METHODOLOGY: An integrative review was performed by the electronic databases: PubMed, Scopus and Web of Science, including studies published between 2020 and 2022, in English and Portuguese languages, using the following descriptors: Sars-CoV-2, Coronavirus, omega-3 fatty acids, eicosapentaenoic acid, inflammation, combined using the Boolean operators AND and OR. RESULTS AND DISCUSSION: Initially, 347 papers were found, and from this total, 11 were selected in this review from the inclusion criteria. The studies showed that there is an inverse relationship between the levels of omega-3 in blood and the patient's clinical status, as well as an improvement in inflammation after supplementation with omega-3 fatty acids. CONCLUSION: Omega-3 fatty acids are present in lower levels in patients with more severe forms of covid-19 and supplementation provided improvement in the inflammatory process, but there is need for further studies to standardize the treatment with omega-3 supplementation. ; INTRODUÇÃO: A covid-19 é uma infecção contagiosa em humanos, cujo quadro clínico pode se apresentar de formas diversas, desde sintomas como febre, congestão nasal e tosse, até manifestações graves como falência de múltiplos órgãos e morte, relacionados ao processo inflamatório exacerbado, característico da doença. O estado nutricional interfere diretamente na susceptibilidade às infecções e também ...
وصف الملف: application/pdf
العلاقة: MEDEIROS, Gislane Batista de Oliveira. Potenciais benefícios dos ácidos graxos ômega-3 frente à covid-19. 2022. 29f. Trabalho de Conclusão de Curso (Graduação em Nutrição), Departamento de Nutrição, Universidade Federal do Rio Grande do Norte, Natal, 2022.; https://repositorio.ufrn.br/handle/123456789/50526Test
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2رسالة جامعية
المؤلفون: Dias, Lêda Karla Monteiro
المساهمون: Maia, Juliana Kelly da Silva, http://lattes.cnpq.br/1134095986257237Test, orcid:0000-0002-7970-534X, http://lattes.cnpq.br/9575492220366110Test, Medeiros, Gidyenne Christine Bandeira Silva de, orcid:0000-0001-5225-385X, http://lattes.cnpq.br/7128174927654982Test, Piuvezam, Grasiela, Batista, Ângela Giovana
مصطلحات موضوعية: Microbiota intestinal, Disbiose, Fitoquímicos, Inflamação, Adiposidade, CNPQ::CIENCIAS DA SAUDE::NUTRICAO
الوصف: Polyphenols can compose the tools for the treatment of obesity through antioxidant, anti-inflammatory and microbiota modulating actions, with effects that widely reach the body, including the intestine. In this way, the systematization of scientific data regarding the effects and mechanisms of action of polyphenols on intestinal health is important to direct obesity coping strategies. The aim of the study was to gather evidence on the impact of polyphenolic compounds on gut health in experimental models of diet-induced obesity. The development of the systematic review was outlined by the Preferred Reporting Items for Systematic Review and Metanalysis (PRISMA). Each step of the review was carried out independently by two researchers. Experimental studies in rats and mice with diet-induced obesity and treated with isolated polyphenols were included, retrieved through the formulation of search strategies applied in the following scientific study databases: PubMed, Science Direct, Scopus, Web of Science and EMBASE. The methodological quality of each selected study was evaluated using the Systematic Review Center for Laboratory Animal Experimentation (SYRCLE) tool by two independent evaluators. There was a high degree of agreement between the evaluators (Kappa coefficient > 0.85). It wasn’t possible to carry out the meta-analysis due to the heterogeneity of the studies in terms of methodology, presentation of results and lack of statistical data. Initially, 1415 publications were retrieved, after applying the eligibility criteria, 20 articles were inserted in the systematic review. Data related to intestinal health (modulation of the microbiota, and other parameters, such as production of short-chain fatty acids, analysis of tight junctions of the intestinal barrier, endotoxemia, morphology and histology and expression of intestinal tissue cytotoxins) were extracted. The SYRCLE assessment showed scores between 4.5 - 7.0 (maximum 10). Data analysis showed clear changes in the intestinal microbiota, such as an ...
وصف الملف: application/pdf
العلاقة: DIAS, Lêda Karla Monteiro. Efeitos dos polifenóis na saúde intestinal de modelos pré-clínicos com obesidade induzida por dieta: revisão sistemática. Orientador: Juliana Kelly da Silva Maia. 2022. 151f. Dissertação (Mestrado em Nutrição) - Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, 2022.; https://repositorio.ufrn.br/handle/123456789/51238Test
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3رسالة جامعية
المؤلفون: Queiroz, Dayanna Joyce Marques
المساهمون: Gonçalves, Maria da Conceição Rodrigues, http://lattes.cnpq.br/0107894093263204Test
مصطلحات موضوعية: Hipovitaminose D, Polimorfismo genético, Fibrose cística, Inflamação, Hypovitaminosis D, Genetic polymorphism, Cystic fibrosis, Inflammation, CNPQ::CIENCIAS DA SAUDE::NUTRICAO
الوصف: Patients with Cystic Fibrosis are at risk for vitamin deficiency due to pancreatic insufficiency. Vitamin deficiency is possibly associated with an inflammatory state and oxidative stress. The presence of genetic polymorphisms in the VDR appears to play a role in supplementation response and clinical effects. The aim of the present study was to evaluate the prevalence of hypovitaminosis D and the influence of the VDR gene’s BsmI polymorphism (rs 1544410) on vitamin D megadose supplementation on inflammatory process and oxidative stress markers in patients with Cystic Fibrosis. A multicenter cross-sectional study was performed, followed by a single-arm nonrandomized pre and post-study with patients who had 25(OH)D insufficiency or deficiency. Patients with Cystic Fibrosis were > 5 years old, both genders. In the first stage of the study, 48 patients with Cystic Fibrosis participated, who were interviewed regarding sociodemographic profile, skin type photo and sun exposure, anthropometric data (weight, height, Body Mass Index), food consumption, biochemical evaluation for analysis of renal function and liver, calcium, parathyroid hormone (PTH), inflammatory process (C-reactive protein (CRP) and alpha-1-acid glycoprotein- A1GPA) and oxidative stress (malondialdehyde- MDA and total antioxidant capacity- CAOT) and genotypic determination of the BsmI polymorphism (rs 1544410). Patients diagnosed with insufficiency and/or vitamin D deficiency, were supplemented with vitamin D, 4000 IU/day for children aged 5 to 10 years and 10,000 IU/day for children over 10 years, adolescents and adults, for 8 weeks. Statistical analysis was performed using the “Statistical Pacage for the Social Sciences” and Prism 6, adopting a significance level of p<0.05. It was observed that the majority of the population had Vitamin D insufficiency and/or deficiency (64.6%). There was no association between vitamin D levels and gender, sun exposure, photo skin type, nutritional status and genotypic variation of the BsmI polymorphism. After ...
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4رسالة جامعية
المؤلفون: Silva, Eliseuda Marinho da
المساهمون: Costa, Maria José de Carvalho, http://lattes.cnpq.br/1587362514258201Test
مصطلحات موضوعية: Nutrição parenteral total, Aminoácidos, Cirurgia, Inflamação, Resultados de cuidados crítico, Evolução clínica, Total parenteral nutrition, Amino acids, Surgery, Inflammation, Critical care results, Clinical evolution, CNPQ::CIENCIAS DA SAUDE::NUTRICAO
الوصف: Low concentrations of glutamine are present in the plasma of patients admitted to the ICU (Intensive Care Unit), with emphasis on major abdominal surgeries, which are associated with increased complications and mortality. Thus, this thesis was intended to evaluate the effect of complementation of the early parenteral alanylglutamine dipeptide on inflammatory markers and prognostic indexes in critically ill patients after major abdominal surgery. It is a randomized controlled double-blind clinical trial consisted of two groups, one named intervention and another named control, made up by individuals who underwent major abdominal surgery and with the use of parenteral nutrition in the Intensive Care Unit of the Hospital University student Lauro Wanderley. Demographic and anthropometric data, prognostic indexes, diagnoses, hematological and biochemical tests, time of intervention, reasons for discontinuing the intervention, length of stay in ICU and hospital, as well as mortality rates, were collected. The sample was composed of 12 patients, 6 patients in each group on a 1: 1 basis in 30 months. Highlighting statistical significance, in the intervention group, it was observed that total serum proteins in the group before the intervention had an average of 4.50 ± 0.87 and after, it was 5.17 ± 0.82 with p = 0.046, serum albumin before the intervention presented an average of 1.97 ± 0.39 and after, it was 2.35 ± 0.63 with p = 0.026, serum CRP (C Reactive Protein) before the intervention presented with an average of 199.36 ± 91.73 and after, it was 132.53 ± 103.81 with p = 0.028 and the CRP/albumin ratio before the intervention presented an avarege of 105.79 ± 49.75 and after, it was 59.76 ± 49.44 with p = 0.028. When considering the intervention group without the two patients who were surgically re-approached, after the intervention, it was observed between the two groups that there was a reduction in blood glucose in the intervention group, with an average of 136.67 ± 58.78 versus 144.00 ± 40.97 in the control group ...
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5رسالة جامعيةEficácia de dietas ocidental e de cafeteria como protocolo para indução de obesidade em ratos Wistar
المؤلفون: Bonfim, Thaís Helena Figueirêdo do
المساهمون: Aquino, Jailane de Souza, http://lattes.cnpq.br/8153908179932184Test
مصطلحات موضوعية: Dietas obesogênicas, Estresse oxidativo, Inflamação, Modelos animais, Síndrome metabólica, Obesogenic diets, Oxidative stress, Inflammation, Animal models, Metabolic syndrome, CNPQ::CIENCIAS DA SAUDE::NUTRICAO
الوصف: Obesity is a increasing public health problem. It is characterized by excess body fat, related to endocrine and metabolic disorders. In recent years, studies have been intensified in order to identify the mechanisms involved in the prevention, development and treatment of obesity, including studies with animal models. Currently there are several methods of inducing obesity in animals, however, diet induction is the one that best mimics the disease. The objective of the present study was to compare the effectiveness of obesity induction by offering new western or cafeteria diets, focusing on the evaluation of metabolic, somatic, oxidative, histological and behavioral parameters of Wistar rats. Initially, 24 male Wistar rats were randomized into three groups: CON: control; WTD: experimental western diet, CAF: experimental cafeteria diet. CON consumed throughout the experiment the AIN93-M diet, the WTD group consumed a diet elaborated with modified AIN93-M ingredients added to highly caloric and palatable foods, and finally the CAF group which consumed, in addition to the AIN93-M diet, industrialized foods arranged on a menu varying in four foods per day. Daily monitoring of food consumption and weekly monitoring of weight was carried out. The duration of the experiment was 107st days. From the 101st to the 103st day of the experiment, behavioral tests were performed. On days 104st and 105st, glucose and insulin tolerance tests were carried out, respectively. At the end of the experiment, the murinometric parameters were checked and the animals euthanized. After the euthanasia, the following analyses were performed: total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein (HDL), triglycerides (TG), aspartate amino transferase (AST), alanine amino transferase (ALT), urea, creatinine, histology of the liver, kidneys and brain, and immunohistochemistry of adipose tissue using interleukin 6 as marker, oxidative parameters in serum, liver, kidneys, brain and body composition. Weight and body mass ...
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6رسالة جامعية
المؤلفون: Martins, Camila Cândida de Lima
المساهمون: Ferreira, Flávia Emília Leite de Lima, http://lattes.cnpq.br/5348717261204017Test
مصطلحات موضوعية: Inflamação, Proteína C-reativa, Adolescente, Consumo de Gorduras, Dieta, Inflammation, C-reactive protein, Adolescents, Fat consumption, Diet, CNPQ::CIENCIAS DA SAUDE::NUTRICAO
الوصف: Introduction: C-reactive protein (CRP) is an inflammatory, acute-phase marker produced in the liver. Doses of ultra-sensitive CRP have been explored as an inflammatory marker, because it has been observed association with arterial alterations in children and adolescents. The association between ultra-sensitive c-reactive protein (hs-CRP) and fat consumption was identified in children, but this relationship is not well established in adolescents. Objective: To evaluate the relationship between the concentrations of hs-CRP and the consumption of total and saturated fats in adolescents, after one year of follow-up. Methods: A longitudinal study conducted in the years 2014 and 2015 evaluated 408 adolescents from municipal and state public schools in João Pessoa, Paraíba, Brazil, between 10 and 14 years of age, participating in the Longitudinal Study on Sedentary Behavior, Physical Activity, Eating Habits and Adolescent Health (LONCAAFS) . Data were obtained on sociodemographic data, anthropometric nutritional status, physical activity and hs-CRP concentration. The consumption of total and saturated fats was evaluated from the 24 hour recall. The associations between concentrations of hs-CRP and total and saturated fat consumption were performed by linear regression considering the panel data, individual fixed effect, balanced bank, stratified by sex and BMI. Results: The mean values of the hs-CRP variable were significantly different between the analyzed years (p = 0.024). The percentage of total and saturated fat consumption is within the recommended level in both years, with no significant difference (p> 0.05). No statistically significant associations were found between hs-CRP and total fat consumption (β = -0.19p = 0.582) and saturated fat (β = 0.20, p = 0.282). Conclusion: The study did not present significant evidence on the relationship between the concentrations of hs-CRP and the consumption of total and saturated fats, as one year of follow-up may not have promoted evident changes in the levels of hs-CRP ...
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7رسالة جامعية
المؤلفون: Assis, Milena Luana Coelho de
المساهمون: Gonçalves, Maria da Conceição Rodrigues, http://lattes.cnpq.br/0107894093263204Test
مصطلحات موضوعية: Fibrose cística, Vitamina D, Polimorfismo, Inflamação, Cystic fibrosis, Vitamin D, Polymorphism, Inflammation, CNPQ::CIENCIAS DA SAUDE::NUTRICAO
الوصف: Cystic fibrosis is an autosomal recessive disease caused by mutations in the CFTR gene. In Brazil, it has an approximate incidence of 1/7000, subject to regional variations. About 90% of patients have pancreatic insufficiency, resulting in malabsorption and risk of deficiency of fat-soluble vitamins, including vitamin D. Vitamin D has recently been linked to several disease states, such as hypertension, diabetes mellitus, cardiovascular disease, Cancer and chronic obstructive pulmonary disease. Vitamin D has immunomodulatory effects on inflammation. Studies have indicated the existence of several polymorphisms in the VDR gene. These polymorphisms have been studied as possible factors involved in predisposition to diseases as well as in the modulation of vitamin D activity. These new insights into the biological functions of vitamin D have led to interest in the clinical consequences of their deficiency. Based on these facts, the study aimed to identify the prevalence of 25- hydroxyvitamin D deficiency / deficiency in individuals with cystic fibrosis and to analyze its association with the inflammatory profile and FokI polymorphism of the VDR gene. This was a cross-sectional study, conducted with patients who were followed at the Pediatric Outpatient Clinic of the University Hospital Lauro Wanderley - UFPB, involving 18 patients, of both sexes. Socio-demographic information, factors associated with sun exposure and clothing were collected through a questionnaire. Weight, height and arm circumference were measured and a food consumption frequency questionnaire was applied. Blood was collected for analysis of biochemical parameters (25-hydroxyvitamin D, parathyroid hormone, serum calcium, alanine transaminase, aspartate transaminase, urea, creatinine, uric acid, fasting glycemia and blood count), inflammatory markers (C-reactive protein, α-1 glycoprotein Acid) and to verify the presence of FokI polymorphism of the vitamin D receptor VDR gene, analyzed by RFLP. Statistical analysis was performed using Student's ...
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8رسالة جامعية
المؤلفون: Toscano, Luciana Tavares
مرشدي الرسالة: Silva, Alexandre Sérgio
المصدر: Biblioteca Digital de Teses e Dissertações da UFPBUniversidade Federal da ParaíbaUFPB.
مصطلحات موضوعية: Chia, Pressão Arterial, Estresse Oxidativo, Inflamação, Modulação Autonômica Cardíaca, Blood Pressure, Oxidative Stress, Inflammation, Cardiac Autonomic Modulation, CNPQ::CIENCIAS DA SAUDE::NUTRICAO
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Hypertension is a multifactorial condition treated by pharmacological and complementary measures such as nutritional approach. In this context, Chia (Salvia hispanica L.) stands out for its nutritional, antioxidant and anti-inflammatory composition. However, it has not yet been elucidated if Chia can reduce blood pressure in hypertensive individuals, and which factors are responsible for this effect. In this context, the present study aimed to verify the effect of supplementation of chia (Salvia hispanica L.) on blood pressure and assess if oxidative stress, inflammation, endothelial function and cardiac autonomic modulation are factors associated with lowering blood pressure in hypertensive individuals treated or not. Thus, a double-blind, randomized controlled trial was conducted with 26 hypertensive individuals. Among these, seventeen treated individuals were randomized to consume chia (CHIA-MD n = 10) and placebo (PLA-MD n = 7). Another group of untreated hypertensive individuals was formed (CHIA-NM, n = 9). They consumed 35 g/day or placebo chia flour 12 weeks. Clinical and ambulatory blood pressure, cardiac autonomic modulation, oxidative stress, inflammation and a marker of nitric oxide production were measured at baseline and after intervention. While PLA-MD group showed no significant change in mean of clinic blood pressure (from 108.0±2.9 to 105.7±2.9 mmHg, p=0.70), it was observed this reduction in GHIA group (from 111.5±1.9 to 102.7±1.5 mmHg, p < 0.001) and CHIA-MD (from 111.3±2.2 to 100.1±1.8 mmHg, p < 0.001), it there was no significant reduction in CHIA-NM (111.7±2.9 para 105.7±2.9 mmHg, p=0.05). In CHIA group, blood pressure reduction was was due to the reduction in both systolic (146.2 ± 2.0 to 136.3 ± 2.6 mmHg, p
A hipertensão arterial sistêmica é uma condição multifatorial que envolve em seu tratamento medidas farmacológicas e complementares, como as nutricionais. Nesse contexto, a Chia (Salvia hispanica L.) se destaca por sua composição nutricional, antioxidante e antiinflamatória. No entanto, ainda não foi elucidado se essa pode reduzir a pressão arterial de indivíduos hipertensos, e quais fatores são responsáveis por este efeito. Nesse contexto, o presente estudo objetivou verificar o efeito da suplementação de chia (Salvia hispanica L.) sobre a pressão arterial e avaliar se estresse oxidativo, inflamação, função endotelial e modulação autonômica cardíaca são fatores associados à redução da pressão arterial em hipertensos medicamentados ou não. Para tanto, um estudo duplo cego, randomizado com placebo controlado foi conduzido com 26 hipertensos. Destes, dezessete tratados medicamentosamente foram randomizados em grupos que consumiriam chia (CHIA-MD; n=10) e placebo (PLA-MD; n=7). Outro grupo de hipertensos não tratados medicamentosamente foi formado (CHIA-NM; n=9). Eles consumiram 35 g/dia de farinha de chia ou placebo durante 12 semanas. Pressão arterial clínica e ambulatorial, modulação autonômica cardíaca, estresse oxidativo, inflamação e marcador de produção de óxido nítrico foram mensurados no período basal e após intervenção. Enquanto o grupo PLA-MD não apresentou alteração significativa da pressão arterial média clínica (108,0±2,9 para 105,7±2,9 mmHg, p=0,70), observou-se esta redução no grupo CHIA (111,5±1,9 para 102,7±1,5 mmHg, p < 0,001) e CHIA-MD (111,3±2,2 para 100,1±1,8 mmHg, p < 0,001), mas sem que houvesse redução significativa em CHIA-NM (111,7±2,9 para 105,7±2,9 mmHg, p=0,05). No grupo CHIA, a redução da pressão média foi decorrente da redução tanto do componente sistólico (146,2±2,0 para 136,3±2,6mmHg, pوصف الملف: application/pdf
العلاقة: 2075167498588264571; 600
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9رسالة جامعية
المؤلفون: Barbosa, Carlos Vinícius da Silva
مرشدي الرسالة: Magnani, Marciane, Silva, Alexandre Sérgio
المصدر: Biblioteca Digital de Teses e Dissertações da UFPBUniversidade Federal da ParaíbaUFPB.
مصطلحات موضوعية: Gergelim, Estresse Oxidativo, Peroxidação lipídica, Inflamação, Dano muscular, Sesame, Oxidative Stress, Lipid peroxidation, Inflammation, Muscle damage, CNPQ::CIENCIAS DA SAUDE::NUTRICAO
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
The strenuous exercise when performed with unbalanced diet and recovery period of the athlete can promote an undesirable state of chronic inflammation and generation of reactive oxygen species (ROS). Those compounds can degrade muscle proteins and contribute to the signs and symptoms of muscle injuries. In recent years, those foods have been tested for their ability to attenuate the deleterious effects in athletes undergoing intense days of training and competition. In this context, sesame (Sesamum indicum L.) stands out for presenting a high nutritional value and recognized antioxidant and anti-inflammatory properties. Considering these aspects, the present study had as its goal to evaluate the effects of the sesame paste consumption (S. indicum L.) on oxidative stress, inflammatory markers and muscle injuries in soccer players. Therefore, a randomized and placebo controlled study was conducted with 19 male soccer athletes, teenagers, with age about 16 years. The sesame paste has been prepared with an addition of 30% of honey from Apis mellifera. The participants were randomly assigned to consume 40 g / d sesame paste or placebo during 28 days of normal training. Before and after intervention were performed anthropometric measurements, functional exams and dosing blood creatine kinase (CK), lactate dehydrogenase (LDH), malondialdehyde (MDA) and high-sensitivity c-reactive protein (hs-CRP) and during a four-week study was performed an evaluation of athletes dietary intake. Biochemical analyzes indicated a physiological unbalanced state of the athletes at the beginning of the experimental procedure. The intake of sesame paste resulted in decreased lipid peroxidation (p=0,002), reduced serum levels of hs-CRP (p=0,010) and LDH (p=0,001), accompanied by an improvement in the aerobic power (p=0.011) and top speed (p=0.005). On the other hand, it was not observed any change of these variables in the placebo group. For anthropometric variables, running speed at the lactate threshold and CK, there were no differences observed in the groups that received sesame paste and placebo after the intervention period. The current study results suggest that the consumption of sesame paste represents an important nutritional strategy by its ability to reduce the inflammatory status, oxidative stress and cause repair of muscle tissue, increasing the physical performance of soccer players, particularly in soccer players previously worn.
O exercício físico intenso quando executado em desequilíbrio com a dieta e período de recuperação do atleta pode promover um indesejável estado crônico de inflamação e geração de espécies reativas de oxigênio (EROs). Estes compostos podem degradar proteínas musculares e contribuir para os sinais e sintomas de lesões musculares. Nos últimos anos alguns alimentos têm sido testados quanto a sua capacidade de atenuar efeitos deletérios em atletas submetidos a jornadas intensas de treinamento e competição. Neste contexto, o gergelim (Sesamum indicum L.) se destaca por apresentar um alto valor nutricional, além de reconhecidas propriedades antioxidantes e antiinflamatórias. Considerando estes aspectos, o presente estudo teve como objetivo avaliar os efeitos do consumo de pasta de gergelim (S. indicum L.) sobre o estresse oxidativo, marcadores inflamatórios e danos musculares em atletas de futebol. Para tanto, um estudo randomizado com placebo controlado foi conduzido com 19 atletas de futebol, adolescentes, do sexo masculino, com idade média de 16 anos. A pasta de gergelim foi elaborada com adição de 30% de mel de Apis mellifera. Os participantes foram aleatoriamente designados a consumir 40 gramas por dia de pasta de gergelim ou placebo durante 28 dias de treinamentos normais. Antes e após a intervenção foram realizadas avaliações antropométricas, testes funcionais e dosagem sanguínea de creatina quinase (CK), lactato desidrogenase (LDH), malondialdeído (MDA) e proteína c-reativa ultra-sensível (PCR-us) e ao longo das quatro semanas do estudo foi realizada avaliação do consumo alimentar dos atletas. As análises bioquímicas indicaram um estado de desequilíbrio fisiológico dos atletas no início do procedimento experimental. A ingestão da pasta de gergelim resultou na diminuição da peroxidação lipídica (p=0,002), redução dos níveis séricos de PCR-us (p=0,010) e LDH (p=0,001), acompanhados por melhora da potência aeróbia (p=0,011) e velocidade pico (p=0,005). Em contrapartida, não foi observada qualquer alteração destas variáveis no grupo placebo. Para as variáveis antropométricas, velocidade de corrida do limiar de lactato e CK não foram observadas diferenças nos grupos que receberam a pasta de gergelim e placebo após o período de intervenção. Os resultados do presente estudo sugerem que o consumo de pasta de gergelim representa uma importante estratégia nutricional devido a sua capacidade de reduzir o estado inflamatório, estresse oxidativo e de induzir reparo do tecido muscular em atletas de futebol, particularmente em atletas de futebol previamente desgastados.وصف الملف: application/pdf
العلاقة: 2075167498588264571; 600
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10رسالة جامعية
المؤلفون: Soares, Geórgia de Sousa Ferreira
مرشدي الرسالة: Gadelha, Tatiane Santi, Siqueira Júnior, José Pinto de
المصدر: Biblioteca Digital de Teses e Dissertações da UFPBUniversidade Federal da ParaíbaUFPB.
مصطلحات موضوعية: Lectina, Abelmoschus esculentus (L.) Moench, Inflamação, Lectin, Inflammation, CNPQ::CIENCIAS DA SAUDE::NUTRICAO
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
The human unconventional or alternative feeding consists of foods or parts not normally used, whose nutritional value has been evaluated over the past years. This made the seeds of various plant species to become alternative sources of proteins and lipids for human consumption. The isolation and physicochemical characterization of new molecules of plant foods, with a view to their physiological action in the human body, are used as potential source for the discovery of new bioactive molecules. A particular group of these molecules, the lectins, are proteins of nonimmune origin containing at least one non-catalytic domain of specific binding, and reversible, the carbohydrates. Several lectins have been studied, both in terms of physicochemical and biological characterization. In this context, okra Abelmoschus esculentus (L.) Moench, (Malvaceae), a vegetable widespread in Northeastern Brazil, besides the easy access and low cost, seems to be a promising source in the search for new lectins. However, research on its purification and isolation are still incipient. The objective of this study was to evaluate the nutritional quality of okra seeds, and to detect, purify, and characterize the physicochemical aspects, and to evaluate the herbal and pharmacological potential ( antinocicepitive,cytotoxic anti-inflammatory and antifungal activity) of the isolated lectin present in Abelmoschus esculentus seeds.showed a predominance of total carbohydrates represented by fibers (30.81%) and soluble carbohydrates (6.69%), proteins (22.14%) and lipids (14.01%). The lectin from seed flour was extracted with 0.1 M Tris pH 7.4 with 0.15 M NaCl. The detection of the presence of lectins AES was determined by testing hemagglutination activity and human erythrocytes shows that B (24.00 UH.mP-1), O (21.00 UH.mP-1) and rabbits treated and untreated (74.41 UH. mP-1) were agglutinated by the lectin.The lectin was purified by precipitation with ammonium sulfate and eluted in ion exchange column. Rabbit erythrocytes were selected for testing the inhibition of the hemagglutinating activity (UA) in carbohydrates. Its hemagglutinating activity was inhibited by lactose, fructose and manose. The protein profile of AES analyzed by PAGE-SDS demonstrates that the lectin is purified, presenting two bands of apparent molecular mass between 25 and 14 kDa. The analysis by mass spectrometry showed the monomeric form with 10.29 kDa and its dimer of 20.58 kDa. The biological activity tests of the lectin from AES flour previously purified presented anti-inflammatory and antinociceptive activity and showed no cytotoxicity when compared to erythrocytes from the ABO system. The intravenous pretreatment of animals with AES (0.01, 0.1 and 1 mg / kg) before the administration of the phlogistic agent (carrageenan) significantly reduced the edema by about 15%, 21.6%, 44%. This inhibition is dose dependent with maximum effect at 1mg/kg. The lectin from Albemoschus esculentus shows antinociceptive activity in writhing test induced by acetic acid and the okra seed extracts are able to recognize and inhibit the growth of the dermatophyte Tricophytum rubrum fungus.
A alimentação humana não convencional ou alternativa é composta por alimentos ou suas partes não habitualmente utilizadas, cujo valor nutritivo vem sendo avaliado ao longo dos últimos anos. Isso fez com que sementes de várias espécies vegetais tornassem recursos alternativos de proteínas e lipídeos para a alimentação humana. O isolamento e a caracterização físico-química de novas moléculas de alimentos vegetais, com vistas à sua ação fisiológica no organismo humano, utilizada como fonte potencial para o descobrimento de novas moléculas bioativas. Um grupo particular dessas moléculas, as lectinas, são proteínas de origem não imune contendo pelo menos um domínio não catalítico de ligação específica, e reversível, a carboidratos. Várias lectinas têm sido estudadas, tanto do ponto de vista de caracterização físico-química quanto biológica. Nesse contexto, o quiabo Abelmoschus esculentus (L.) Moench, (Malvaceae), hortaliça bastante difundida na região Nordeste do Brasil, além de fácil acesso e baixo custo, apresenta-se como uma fonte bastante promissora na busca por novas lectinas. Entretanto, pesquisas relativas à sua purificação e ao seu isolamento ainda são incipientes. Objetiva-se com este trabalho avaliar a qualidade nutricional das sementes do quiabo, detectar, purificar e caracterizar físico-quimicamente, avaliar o potencial fitoterápico e farmacológico (antinocicepitivo, citotóxico, antiinflamatório e antifúngico) da lectina isolada de sementes de Abelmoschus esculentus. As sementes apresentaram uma predominância de carboidratos totais representados pelas fibras (30,81%) e carboidratos solúveis (6,69%), proteinas (22,14%) e lipídeos (14,01%). A lectina da farinha das sementes foi extraída com Tris 0,1M pH 7,4 com NaCl 0,15M. A detecção da existência de lectinas (AES) foi determinada através de ensaio de atividade hemaglutinante e revela que eritrócitos humanos B (24.00 UH.mP-1), O (21.00 UH.mP-1) e coelho tratado e não tratado (74.41 UH.mP-1) foram aglutinados pela lectina. A lectina foi purificada por precipitação com sulfato de amônio e purificada em coluna de troca iônica. Eritrócitos de coelho foram utilizados para os testes de inibição da atividade hemaglutinante (UH) com carboidratos. Sua atividade hemaglutinante foi inibida por lactose, frutose e manose. O perfil protéico da AES analisado por PAGE-SDS demonstra que a lectina encontra-se purificada, apresentando duas bandas de massa molecular aparente entre 25 e 14 kDa. A análise por espectrometria de massa mostrou a forma monomérica com 10,29 kDa e seu dímero de 20,58 kDa. Os testes de atividade biológica da lectina de farinha de sementes previamente purificada apresentaram atividade antiinflamatória, antinociceptiva e não apresentou citotoxicidade frente a hemácias do sistema ABO. O pré-tratamento endovenoso dos animais com a AES (0,01; 0,1 e 1mg/kg), antes da administração do agente flogistico (carragenina), reduziu significativamente o edema em cerca de 15%, 21,6%, 44%. Esta inibição é dose dependente tendo seu máximo efeito na dose de 1mg/kg. A lectina de Abelmoschus esculentus apresenta atividade antinociceptiva no teste de contorções abdominais induzidas por ácido acético e os extratos da semente de quiabo são capazes de reconhecer e inibir o crescimento do fungo dermatófito Tricophytum rubrum.وصف الملف: application/pdf
العلاقة: 2075167498588264571; 600
