دورية أكاديمية
Respiratory viral co-infections among SARS-CoV-2 cases confirmed by virome capture sequencing
| العنوان: | Respiratory viral co-infections among SARS-CoV-2 cases confirmed by virome capture sequencing |
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| المؤلفون: | Kim, KW, Deveson, IW, Pang, CNI, Yeang, M, Naing, Z, Adikari, T, Hammond, JM, Stevanovski, I, Beukers, AG, Verich, A, Yin, S, McFarlane, D, Wilkins, MR, Stelzer-Braid, S, Bull, RA, Craig, ME, van Hal, SJ, Rawlinson, WD |
| المصدر: | urn:ISSN:2045-2322 ; Scientific Reports, 11, 1, 3934 |
| بيانات النشر: | Springer Nature |
| سنة النشر: | 2021 |
| المجموعة: | UNSW Sydney (The University of New South Wales): UNSWorks |
| مصطلحات موضوعية: | Infectious Diseases, Biodefense, Pneumonia & Influenza, Pneumonia, Emerging Infectious Diseases, Vaccine Related, Lung, Prevention, Infection, 3 Good Health and Well Being, Australia, COVID-19, Coinfection, Computational Biology, Genome, Viral, Humans, Open Reading Frames, Reproducibility of Results, SARS-CoV-2, Sequence Analysis, DNA, Virome, Whole Genome Sequencing |
| الوصف: | Accumulating evidence supports the high prevalence of co-infections among Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) patients, and their potential to worsen the clinical outcome of COVID-19. However, there are few data on Southern Hemisphere populations, and most studies to date have investigated a narrow spectrum of viruses using targeted qRT-PCR. Here we assessed respiratory viral co-infections among SARS-CoV-2 patients in Australia, through respiratory virome characterization. Nasopharyngeal swabs of 92 SARS-CoV-2-positive cases were sequenced using pan-viral hybrid-capture and the Twist Respiratory Virus Panel. In total, 8% of cases were co-infected, with rhinovirus (6%) or influenzavirus (2%). Twist capture also achieved near-complete sequencing (> 90% coverage, > tenfold depth) of the SARS-CoV-2 genome in 95% of specimens with Ct < 30. Our results highlight the importance of assessing all pathogens in symptomatic patients, and the dual-functionality of Twist hybrid-capture, for SARS-CoV-2 whole-genome sequencing without amplicon generation and the simultaneous identification of viral co-infections with ease. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | http://hdl.handle.net/1959.4/unsworks_74309Test; https://unsworks.unsw.edu.au/bitstreams/304ce843-9e8d-43cf-8c17-17e6ee3e7df4/downloadTest; https://doi.org/10.1038/s41598-021-83642-xTest |
| DOI: | 10.1038/s41598-021-83642-x |
| الإتاحة: | https://doi.org/10.1038/s41598-021-83642-xTest http://hdl.handle.net/1959.4/unsworks_74309Test https://unsworks.unsw.edu.au/bitstreams/304ce843-9e8d-43cf-8c17-17e6ee3e7df4/downloadTest |
| حقوق: | open access ; https://purl.org/coar/access_right/c_abf2Test ; CC BY ; https://creativecommons.org/licenses/by/4.0Test/ ; free_to_read |
| رقم الانضمام: | edsbas.805022A6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-021-83642-x |
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