المؤلفون: Xue-Zhen Cao, Jin-Lei Wang, Hany M. Elsheikha, Ting-Ting Li, Li-Xiu Sun, Qin-Li Liang, Zhi-Wei Zhang, Rui-Qing Lin

المصدر: Frontiers in Cellular and Infection Microbiology, Vol 9 (2019)

مصطلحات موضوعية: 0301 basic medicine, Microbiology (medical), 030106 microbiology, Immunology, Mutant, lcsh:QR1-502, Virulence, Toxoplasma gondii, Microbiology, lcsh:Microbiology, 03 medical and health sciences, brain cyst burden, CRISPR/Cas9, Infectivity, Virus quantification, biology, Aa16GL, biology.organism_classification, Molecular biology, In vitro, virulence, 030104 developmental biology, Infectious Diseases, Intracellular, Immunostaining

الوصف: In this study, we characterized the role of amylo-alpha-1,6-glucosidase (Aa16GL) in the biology and infectivity of Toxoplasma gondii, using Aa16GL-deficient parasites of type I RH and type II Prugniaud (Pru) strains. The subcellular localization of Aa16GL protein was characterized by tagging a 3 × HA to the 3' end of the Aa16GL gene endogenous locus. Immunostaining of the expressed Aa16GL protein revealed that it is located in several small cytoplasmic puncta. Functional characterization of ΔAa16GL mutants using plaque assay, egress assay and intracellular replication assay showed that parasites lacking Aa16GL exhibit a slight reduction in the growth rate, but remained virulent to mice. Although PruΔAa16GL tachyzoites retained the ability to differentiate into bradyzoites in vitro, they exhibited slight reduction in their ability to form cysts in mice. These findings reveal new properties of Aa16GL and suggest that while it does not have a substantial role in mediating T. gondii infectivity, this protein can influence the formation of parasite cysts in mice.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c428fe04b107c5ee78216fe6fc4d44bTest
https://www.frontiersin.org/article/10.3389/fcimb.2019.00418/fullTest

المؤلفون: Ting-Ting Li, Jin-Lei Wang, Nian-Zhang Zhang, Wen-Hui Li, Hong-Bin Yan, Li Li, Wan-Zhong Jia, Bao-Quan Fu

المصدر: Frontiers in Cellular and Infection Microbiology
Frontiers in Cellular and Infection Microbiology, Vol 9 (2019)

مصطلحات موضوعية: 0301 basic medicine, Microbiology (medical), Time Factors, Trichinella, 030106 microbiology, Immunology, lcsh:QR1-502, Vertebrate Animals, Recombinase Polymerase Amplification, Microbiology, Sensitivity and Specificity, lcsh:Microbiology, 03 medical and health sciences, chemistry.chemical_compound, Mice, Cellular and Infection Microbiology, rapid test, parasitic diseases, lateral flow strip, Methods, diagnostics, Animals, Humans, recombinase polymerase amplification, Gene, High concentration, biology, fungi, Trichinellosis, Ribosomal RNA, biology.organism_classification, Molecular biology, 030104 developmental biology, Infectious Diseases, Visual detection, chemistry, Molecular Diagnostic Techniques, Nucleic Acid Amplification Techniques, DNA

الوصف: Trichinella spp., are amongst the most widespread parasitic nematodes, primarily live in the muscles of a wide range of vertebrate animals and humans. Human infection occurs by ingestion of raw or undercooked meat containing Trichinella larvae. Accurate diagnosis of Trichinella spp. infection in domestic animals is crucial for the effective prevention and control of human trichinellosis. In the present study, a simple, rapid and accurate diagnostic assay was developed combining recombinase polymerase amplification and a lateral flow strip (LF-RPA) to detect Trichinella spp. infection. The LF-RPA assay targets Trichinella spp. mitochondrial small-subunit ribosomal RNA (rrnS) gene and can detect as low as 100 fg DNA of Trichinella strains, which was approximately 10 times more sensitive than a conventional PCR assay. The LF-RPA assay can be performed within 10–25 min, at a wide range of temperatures (25–45°C) and showed no cross-reactivity with DNA of other parasites and related host species of Trichinella. The performance of the LF-RPA assay in the presence of high concentration of PCR inhibitor was better than that of a conventional PCR assay. Results obtained by LF-RPA assay for the detection of experimentally infected mice were comparable to the results obtained by using a conventional PCR, achieving 100% specificity and high sensitivity. These results present the developed LF-RPA assay as a new simple, specific, sensitive, rapid and convenient method for the detection of Trichinella infection in domestic animals.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11902cf67dedbb4d018518b327a4f9ecTest
http://europepmc.org/articles/PMC6348712Test

المؤلفون: Jin-Lei Wang, Jun-Jun He, Min-Jun Xu, Jun Ma, Xing-Quan Zhu

المصدر: Infection, Genetics and Evolution. 37:137-142

مصطلحات موضوعية: 0301 basic medicine, Microbiology (medical), Microbiology, Mice, 03 medical and health sciences, microRNA, Genetics, Animals, Gene Regulatory Networks, KEGG, Molecular Biology, Gene, Ecology, Evolution, Behavior and Systematics, Regulation of gene expression, biology, Sequence Analysis, RNA, Gene Expression Profiling, Wnt signaling pathway, High-Throughput Nucleotide Sequencing, Toxoplasma gondii, biology.organism_classification, Actin cytoskeleton, Gene expression profiling, MicroRNAs, 030104 developmental biology, Infectious Diseases, Gene Expression Regulation, Immunology, Cancer research, Spleen, Toxoplasmosis

الوصف: Toxoplasma gondii is a worldwide prevalent pathogen that infects most of the warm-blood vertebrates. To investigate the regulation network of splenic miRNAs altered by acute infection with T. gondii, we herein investigated the changes of miRNA profile in mouse spleen via next generation sequencing and bioinformatics analysis. A total of 379 miRNAs was identified, 131 miRNAs of them were differentially expressed (including 97 upregulated and 34 downregulated miRNAs). 48 differentially expressed miRNAs had validated targets in the miRWalk2.0 database. Gene Ontology (GO) enrichment analysis revealed that the validated targets of differently expressed miRNAs were significantly enriched in gene transcription regulation. It suggested that T. gondii can modulate host gene expression through targeting to trans-regulation factors. The genes involved in apoptosis or anti-apoptosis were both targeted by differentially expressed miRNAs. The change of power balance between the miRNAs targeting host apoptosis genes and those regulating host anti-apoptosis genes contributes to the fate of host apoptosis process. Twelve pathways were significantly enriched in KEGG analysis with most of them being cancer related, including pathways in cancer, pancreatic cancer, colorectal cancer, axon guidance, MAPK signaling pathway, focal adhesion, chronic myeloid leukemia, renal cell carcinoma, prostate cancer, glioma, regulation of actin cytoskeleton, and Wnt signaling pathway. Our study showed a changed miRNA regulation network in mouse spleen infected by T. gondii. These findings will be helpful for better understanding of miRNA regulation network in host-T. gondii interaction, revealing the relationship among T. gondii infection, gene regulation, apoptosis and cancer process alterations in infected spleen.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ecddd794b9ff9b0aa0b9a3d0bd1349e7Test
https://doi.org/10.1016/j.meegid.2015.11.005Test

المؤلفون: Jin-Lei Wang, Si-Yang Huang, Xing-Quan Zhu, Kai Chen, Shou-Feng Zhang, Rongliang Hu, Qiu-Xia Yao, Quan Liu

المصدر: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. 52

مصطلحات موضوعية: 0301 basic medicine, Microbiology (medical), China, Protozoan Proteins, Locus (genetics), Microbiology, 03 medical and health sciences, parasitic diseases, Genotype, Genetics, Mustelidae, Prevalence, Animals, Molecular Biology, Gene, Ecology, Evolution, Behavior and Systematics, biology, Toxoplasma gondii, DNA, Protozoan, biology.organism_classification, Virology, genomic DNA, 030104 developmental biology, Infectious Diseases, Melogale moschata, Genetic marker, Restriction fragment length polymorphism, Multiplex Polymerase Chain Reaction, Toxoplasma, Polymorphism, Restriction Fragment Length

الوصف: Toxoplasma gondii is an obligate intracellular apicomplexan parasite which is able to infect almost all warm-blooded animals. There is no information about the prevalence and genetic characterization of T. gondii in badgers (Melogale moschata) in China. Here, a total of 367 badgers were captured from different cities in Jiangxi province, Southern China. Genomic DNA was extracted from brain tissues of each badgers, and 57 (15.45%) of them were positive for T. gondii by semi-nested PCR of the B1 gene. The positive DNA samples were typed at 11 genetic markers, including 10 nuclear loci (SAG1, 5'-SAG2 and 3'-SAG2, alternative SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1) and an apicoplast locus (Apico), with multilocus polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology. Among them, 4 were completely typed at all loci, and 2 was genotyped for 9 loci, showing that they belong to ToxoDB#9. This is the first report of prevalence and genetic characterization of T. gondii isolates from badgers in China, which contributes to broader understanding of population structure of T. gondii in China. It is important for the prevention and control of T. gondii infection in wild animals.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb4381a0efbc36ed397586510b05a47bTest
https://pubmed.ncbi.nlm.nih.gov/28434971Test