المؤلفون: Boudjemaa, Rym, Steenkeste, Karine, Jacqueline, Cédric, Briandet, Romain, Caillon, Jocelyne, Boutoille, David, Le Mabecque, Virginie, Tattevin, Pierre, Fontaine-Aupart, Marie-Pierre, Revest, Matthieu

المساهمون: Institut des Sciences Moléculaires d'Orsay (ISMO), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Thérapeutiques cliniques et expérimentales des infections (EA 3826) (EA 3826), Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, CHU Pontchaillou Rennes, ARN régulateurs bactériens et médecine (BRM), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Collège des enseignants des Maladies Infectieuses et Tropicales, 2014-172, Ministère de l’Education Nationale et de la Recherche, Université Paris-Sud

المصدر: ISSN: 0305-7453.

مصطلحات موضوعية: antibiotics, macrophage, bacteria, confocal microscopy, mice, antibiotic therapy, prostheses, staphylococcus aureus, host (organism), biofilms, infection, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases

الوصف: International audience ; Objectives: To evaluate the significant role played by biofilms during prosthetic vascular material infections (PVMIs).Methods: We developed an in vivo mouse model of Staphylococcus aureus PVMI allowing its direct observation by confocal microscopy to describe: (i) the structure of biofilms developed on Dacron® vascular material; (ii) the localization and effect of antibiotics on these biostructures; and (iii) the interaction between bacteria and host tissues and cells during PVMI.Results: In this model we demonstrated that the biofilm structures are correlated to the activity of antibiotics. Furthermore, live S. aureus bacteria were visualized inside the macrophages present at the biofilm sites, which is significant as antibiotics do not penetrate these immune cells.Conclusions: This intracellular situation may explain the limited effect of antibiotics and also why PVMIs can relapse after antibiotic therapy.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/29901764; hal-01834453; https://univ-rennes.hal.science/hal-01834453Test; https://univ-rennes.hal.science/hal-01834453v2/documentTest; https://univ-rennes.hal.science/hal-01834453v2/file/Boudjemaa_Live%20intramacrophagic%20Staphylococcus-final-version.pdfTest; PRODINRA: 449901; PUBMED: 29901764; WOS: 000443539100020

الإتاحة: https://doi.org/10.1093/jac/dky205Test
https://univ-rennes.hal.science/hal-01834453Test
https://univ-rennes.hal.science/hal-01834453v2/documentTest
https://univ-rennes.hal.science/hal-01834453v2/file/Boudjemaa_Live%20intramacrophagic%20Staphylococcus-final-version.pdfTest

المؤلفون: Bot, Audrey Le, Lecomte, Raphaël, Gazeau, Pierre, Benezit, François, Arvieux, Cédric, Ansart, Séverine, Boutoille, David, Berre, Rozenn Le, Chabanne, Céline, Lesouhaitier, Matthieu, Dejoies, Loren, Flecher, Erwan, Chapplain, Jean-Marc, Tattevin, Pierre, Revest, Matthieu

المصدر: Clinical Infectious Diseases; 5/1/2021, Vol. 72 Issue 9, pe249-e255, 7p

مصطلحات موضوعية: RESEARCH, HEART valve diseases, LENGTH of stay in hospitals, CEREBRAL embolism & thrombosis, SCIENTIFIC observation, CONFIDENCE intervals, MULTIVARIATE analysis, MEDICAL cooperation, RETROSPECTIVE studies, TREATMENT duration, INFECTIVE endocarditis, TREATMENT effectiveness, INFECTION, STAPHYLOCOCCAL diseases, DISEASE relapse, STAPHYLOCOCCUS aureus, DESCRIPTIVE statistics, RIFAMPIN, ODDS ratio, COMPLICATIONS of prosthesis, LONGITUDINAL method

مستخلص: Background International guidelines recommend rifampin-based combinations for staphylococcal prosthetic valve endocarditis (PVE). However, no robust clinical data support this recommendation, and rifampin tolerability is an issue. We aimed to evaluate the impact of rifampin for the treatment of staphylococcal PVE. Methods An observational retrospective cohort study of all adults with staphylococcal PVE (modified Duke criteria) was conducted in 3 referral centers for endocarditis, during years 2000–2018. Primary outcome measurement was 1-year mortality. Results We enrolled 180 patients with PVE due to Staphylococcus aureus (n = 114, 63.3%), or coagulase-negative staphylococci (n = 66, 36.7%), on bioprosthesis (n = 111, 61.7%), mechanical valve (n = 67, 37.2%), or both (n = 2). There were 132 males (73.3%), and mean age was 70.4 ± 12.4 years. Valvular surgery was performed in 51/180 (28.3%) cases. Despite all isolates were susceptible to rifampin, only 101 (56.1%) were treated with rifampin, for a median duration of 33.0 days, whereas 79 (43.9%) received no rifampin. Baseline characteristics were similar in both groups. One-year mortality was, respectively, 37.6% (38/101), and 31.6% (25/79), in patients treated with, or without, rifampin (P =.62). Relapse rates were 5.9% (6/101), and 8.9% (7/79), P =.65. Patients treated with rifampin had longer hospital length-of-stay: 42.3 ± 18.6 vs 31.3 ± 14.0 days (P <.0001). On multivariate analysis, only cerebral emboli (odds ratio [OR] 2.95, 95% confidence interval [CI], 1.30–6.70, P =.009), definite endocarditis (OR 7.15, 95% CI, 1.47–34.77, P =.018), and methicillin-resistant S. aureus (OR 6.04, 95% CI, 1.34–27.26, P =.019), were associated with 1-year mortality. Conclusions A large proportion (43.9%) of staphylococcal PVE received no rifampin. One-year survival and relapse rates were similar in patients treated with or without rifampin. [ABSTRACT FROM AUTHOR]

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المؤلفون: Pol, Stanislas, Bonnet, Delphine, Payssan‐Sicart, Virginie, Pomes, Chloe, Bailly, François, Beaudoin, Marjolaine, Giboz, Dominique, Hartig‐Lavie, Kerstin, Maynard, Marianne, Billaud, Eric, Boutoille, David, Cavellec, Morane, Chevalier, Caroline, Hubert, Isabelle, Goepfert, Pierre, Lannes, Adrien, Lunel, Françoise, Boursier, Jérôme, Boyer, Nathalie, Giuily, Nathalie

المصدر: Alimentary Pharmacology & Therapeutics; Mar2021, Vol. 53 Issue 5, p616-629, 14p, 2 Diagrams, 3 Charts

مصطلحات موضوعية: CHRONIC hepatitis B, CHOLANGITIS, HEPATITIS B virus, LIVER transplantation, HEPATOCELLULAR carcinoma, INFECTION

مستخلص: Summary: Background: Chronic hepatitis B virus (HBV) infection results in a high risk of cirrhosis and its complications, cirrhosis decompensation (DC), hepatocellular carcinoma (HCC), liver transplantation (LT), death or any of these outcomes (composite endpoint [CE]). Nucleos(t)ide analogues (NUCs) such as tenofovir or entecavir are associated with a reduction in these complications. Aim: To compare the impact of tenofovir and entecavir on these outcomes in patients treated for HBV infection and included in the prospective Hepather cohort. Methods: All patients with HBV infection who had received tenofovir or entecavir for more than 6 months at or after entry in the ANRS CO22 cohort were selected. Patients with HDV and HCV co‐infection or prior liver event were excluded. Incidence rates of events were compared using inverse probability of treatment weighting (IPW). Results: The cohort included 1800 patients (986 tenofovir and 814 entecavir). Median follow‐up was 4.2 years. The incidences of HCC, DC, LT, ACD, LRD and CE were not different between tenofovir‐ (1.8 (0.9; 3.2), 0.6 (0.2; 1.6), 0.2 (0.0; 0.8), 1.7 (0.8; 3.0), 0.8 (0.2, 1.8) and 4.1 (3.0; 5.4) per 1000 person‐years) and entecavir‐treated patients (1.6 (0.7; 3.0), 0.7 (0.2; 1.8), 0.2 (0.0; 1.0), 3.0 (1.7, 4.8), 0.5 (0.1; 1.5) and 5.0 (3.3; 7.2)) per 1000 person‐years, respectively. Conclusion: The risk of liver‐related events or death was not different between tenofovir‐ and entecavir‐treated patients in this large prospective cohort of predominantly non‐cirrhotic French patients. Trial registration number: NCT019553458. [ABSTRACT FROM AUTHOR]

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المؤلفون: Lecomte, Raphaël, Laine, Jean-Baptiste, Issa, Nahéma, Revest, Matthieu, Gaborit, Benjamin, Turnier, Paul Le, Deschanvres, Colin, Benezit, François, Asseray, Nathalie, Tourneau, Thierry Le, Pattier, Sabine, Habash, Ousama Al, Raffi, François, Boutoille, David, Camou, Fabrice

المصدر: Clinical Infectious Diseases; Sep2020, Vol. 71 Issue 5, p1316-1319, 4p

مصطلحات موضوعية: PROSTHETIC heart valves, INFECTION, INFECTIVE endocarditis, COMPLICATIONS of prosthesis, ENTEROCOCCAL infections, DISEASE relapse, TREATMENT effectiveness, DESCRIPTIVE statistics

مستخلص: In nonoperated prosthetic valve endocarditis (PVE), long-term outcome is largely unknown. We report the follow-up of 129 nonoperated patients with PVE alive at discharge. At 1 year, the mortality rate was 24%; relapses and reinfection were rare (5% each). Enterococcal PVE was associated with a higher risk of relapse. [ABSTRACT FROM AUTHOR]

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المؤلفون: Leroy, Anne-Gaëlle, Corvec, Stéphane, Danneels, Pierre, Dubée, Vincent, Kempf, Marie, Boucher, Delphine, Issa, Nahéma, Peuchant, Olivia, Camou, Fabrice, Boutoille, David, Lecomte, Raphaël

المصدر: Clinical Infectious Diseases; 01/15/2021, Vol. 72 Issue 2, p361-362, 2p

مصطلحات موضوعية: PROSTHETIC heart valves, INFECTION, INFECTIVE endocarditis, COMPLICATIONS of prosthesis, ENTEROCOCCAL infections, DISEASE relapse, TREATMENT effectiveness

المؤلفون: Lecomte, Raphaël, Issa, Nahéma, Camou, Fabrice, Boutoille, David

المصدر: Clinical Infectious Diseases; Dec2020, Vol. 71 Issue 11, p3011-3012, 2p

مصطلحات موضوعية: PROSTHETIC heart valves, INFECTION, INFECTIVE endocarditis, COMPLICATIONS of prosthesis, DISEASE relapse, TREATMENT effectiveness

المؤلفون: Henry, Benoît, Corvec, Stéphane, Crémet, Lise, Guillouzouic, Aurélie, Marraillac, Julie, Juvin, Marie-Emmanuelle, Touchais, Sophie, Asseray, Nathalie, Boutoille, David, Reynaud, Alain, Bémer, Pascale

المصدر: Scandinavian Journal of Infectious Diseases; Aug2012, Vol. 44 Issue 8, p610-614, 5p, 2 Charts

مصطلحات موضوعية: ACADEMIC medical centers, CHROMOSOME banding, DRUG resistance in microorganisms, GENES, HISTOLOGICAL techniques, INFECTION, MICROBIAL sensitivity tests, COMPLICATIONS of prosthesis, PULSED-field gel electrophoresis, STAPHYLOCOCCUS, TOTAL hip replacement, TOTAL knee replacement, PHENOTYPES, DISEASE relapse, RETROSPECTIVE studies, SUPERINFECTION, DATA analysis software, DESCRIPTIVE statistics

مستخلص: Twenty-one isolates of Staphylococcus epidermidis from 9 patients with persistent prosthetic joint infections were analysed by pulsed-field gel electrophoresis and antibiotic susceptibility assays. In 7 of these cases, the S. epidermidis isolate was different from that of the initial episode. In 1 further case, the superinfection was polyclonal. Recurrence, i.e., renewed isolation of a clone identical to that of an initial episode, occurred in 3 cases, 1 of which was in the absence of superinfection. A high degree of antibiotic resistance was demonstrated, including methicillin in 17 of 21 strains. In conclusion, a frequent occurrence of superinfection and a high degree of resistance make management of these infections complex. [ABSTRACT FROM AUTHOR]

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المؤلفون: Asseray, Nathalie, Papin, Christophe, Touchais, Sophie, Bemer, Pascale, Lambert, Chantal, Boutoille, David, Tequi, Brigitte, Gouin, François, Raffi, François, Passuti, Norbert, Potel, Gilles

المصدر: Scandinavian Journal of Infectious Diseases; 2010, Vol. 42 Issue 6-7, p421-425, 5p, 1 Diagram, 3 Charts

مصطلحات موضوعية: CUTIBACTERIUM acnes, OSTEOMYELITIS diagnosis, RETROSPECTIVE studies, DIFFERENTIAL diagnosis, INFECTION, DIAGNOSIS

مستخلص: The identification of Propionibacterium acnes in cultures of bone and joint samples is always difficult to interpret because of the ubiquity of this microorganism. The aim of this study was to propose a diagnostic strategy to distinguish infections from contaminations. This was a retrospective analysis of all patient charts of those patients with ≥1 deep samples culture-positive for P. acnes. Every criterion was tested for sensitivity, specificity, and positive likelihood ratio, and then the diagnostic probability of combinations of criteria was calculated. Among 65 patients, 52 (80%) were considered truly infected with P. acnes, a diagnosis based on a multidisciplinary process. The most valuable diagnostic criteria were: ≥2 positive deep samples, peri-operative findings (necrosis, hardware loosening, etc.), and ≥2 surgical procedures. However, no single criterion was sufficient to ascertain the diagnosis. The following combinations of criteria had a diagnostic probability of >90%: ≥2 positive cultures + 1 criterion among: peri-operative findings, local signs of infection, ≥2 previous operations, orthopaedic devices; 1 positive culture + 3 criteria among: peri-operative findings, local signs of infection, ≥2 previous surgical operations, orthopaedic devices, inflammatory syndrome. The diagnosis of P. acnes osteomyelitis was greatly improved by combining different criteria, allowing differentiation between infection and contamination. [ABSTRACT FROM AUTHOR]

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المؤلفون: Gillard, Jérôme¹, Boutoille, David², Varin, Stéphane¹, Asseray, Nathalie³, Berthelot, Jean-Marie¹ jeanmarie.berthelot@chu-nantes.fr, Maugars, Yves¹

المصدر: Joint Bone Spine. Mar2005, Vol. 72 Issue 2, p156-162. 7p.

مصطلحات موضوعية: *IATROGENIC diseases, *THERAPEUTIC complications, *MICROBIOLOGY, *ABSCESSES, *ENDOCARDITIS, *DIABETES

مستخلص: Unlabelled: Few data are available on patients with suspected noniatrogenic pyogenic discitis but negative microbiological tests.Objectives: To compare the features, treatment, and outcomes in patients with suspected versus microbiologically documented noniatrogenic pyogenic discitis.Patients: A retrospective chart review identified eight patients with suspected noniatrogenic pyogenic discitis managed at our institution over a 15-year period. Eighteen age- and sex-matched patients with microbiologically documented noniatrogenic pyogenic discitis managed at our institution during the same period served as controls.Results: The eight cases had a longer time to diagnosis, a greater risk of abscess formation or epidural infection, and a smaller number of cutaneous portals of entry, as compared to the controls. None of the cases had evidence of endocarditis or diabetes mellitus. Infection at another site preceded the discitis in half the cases. Empirical treatment with two antimicrobials (usually a fluoroquinolone with a beta-lactam or fosfomycin) ensured a full recovery in all eight cases, with no relapses or long-term recurrences, whereas relapses occurred in three of the 18 controls treated with antibiotics selected by antibiotic susceptibility testing.Conclusion: Noniatrogenic pyogenic discitis with negative microbiological studies is associated with distinctive clinical features consistent with smoldering infection. The outcome is favorable under empirical two-drug antimicrobial therapy, usually including a fluoroquinolone given by the intravenous route. [ABSTRACT FROM AUTHOR]

المؤلفون: Varenne, Yoann, Corvec, Stéphane, Leroy, Anne-Gaëlle, Boutoille, David, Nguyễn, Mỹ-Vân, Touchais, Sophie, Bémer, Pascale, Hamel, Antoine, Waast, Denis, Nich, Christophe, Gouin, François, Crenn, Vincent

المصدر: Antibiotics (2079-6382); Jul2021, Vol. 10 Issue 7, p768, 1p

مصطلحات موضوعية: TUMOR surgery, PELVIC bones, ANTIBIOTIC prophylaxis, SURGICAL site infections, DRUG resistance in bacteria, INFECTION, BACTERIAL ecology

مستخلص: Resections of primary pelvic bone tumors are frequently complicated by surgical site infections (SSIs), thereby impairing the functional prognosis of patients, especially in case of implant removal. Although prophylactic antibiotics play an essential role in preventing SSIs, there are presently no recommendations that support their appropriate use. This study aimed to assess the impact of a 24 h prophylactic protocol on the bacterial ecology, the resistance pattern, and the SSI healing rate. We hypothesized that this protocol not only limits the emergence of resistance but also results in a good cure rate with implant retention in case of SSI. A retrospective study was performed that included all patients with an SSI following a pelvic bone tumoral resection between 2005 and 2017 who received a 24 h antibiotic prophylaxis protocol. Twenty-nine patients with an SSI were included. We observed a 75.9% rate of polymicrobial infection, with a high prevalence of digestive flora microorganisms and a majority of wild-type phenotypes. We confirmed that there was no significant emergence of resistant flora. After first-line debridement, antibiotics (DA) if any implant was used, or debridement, antibiotics, and implant retention (DAIR) whenever possible, we obtained a 79.3% cure rate, with implant removal in 20% of cases. The absence of an implant was significantly associated with SSI healing. Early infection management and low resistance profiles may also have a positive effect, but this needs to be confirmed in a larger cohort. In light of this, the use of a 24 h prophylactic protocol in primary pelvic bone tumor resections is associated with a favorable infection cure rate and implant retention in case of SSI, and minimal selection of resistant microorganisms. [ABSTRACT FROM AUTHOR]

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