التفاصيل البيبلوغرافية
| العنوان: |
Sphingosine 1-phosphate receptor 3 regulates recruitment of anti-inflammatory monocytes to microvessels during implant arteriogenesis. |
| المؤلفون: |
Awojoodu, Anthony O., Ogle, Molly E., Sefcik, Lauren S., Bowers, Daniel T., Martin, Kyle, Brayman, Kenneth L., Lynch, Kevin R., Peirce-Cottler, Shayn M., Botchwey, Edward |
| المصدر: |
Proceedings of the National Academy of Sciences of the United States of America; 8/20/2013, Vol. 110 Issue 34, p13785-13790, 6p |
| مصطلحات موضوعية: |
SPHINGOSINE-1-phosphate, SPHINGOLIPIDS, ENDOTHELIAL cells, BIOMATERIALS, IMMUNOREGULATION, TISSUE engineering |
| مستخلص: |
Endothelial cells play significant roles in conditioning tissues after injury by the production and secretion of angiocrine factors. At least two distinct subsets of monocytes, CD45+CD11b+Gr1+Ly6C+ inflammatory and CD45+CD11b+Gr1-Ly6C- anti-inflammatory monocytes, respond differentially to these angiocrine factors and promote pathogen/debris clearance and arteriogenesis/tissue regeneration, respectively. We demonstrate here that local sphingosine 1-phosphate receptor 3 (S1P3) agonism recruits anti-inflammatory monocytes to remodeling vessels. Poly(lactic-co-glycolic acid) thin films were used to deliver FTY720, an S1P1/3 agonist, to inflamed and ischemic tissues, which resulted in a reduction in proinflammatory cytokine secretion and an increase in regenerative cytokine secretion. The altered balance of cytokine secretion results in preferential recruitment of anti-inflammatory monocytes from circulation. The chemotaxis of these cells, which express more S1P3 than inflammatory monocytes, toward SDF-1α was also enhanced with FTY720 treatment, but not in S1P3 knockout cells. FTY720 delivery enhanced arteriolar diameter expansion and increased length density of the local vasculature. This work establishes a role for S1P receptor signaling in the local conditioning of tissues by angiocrine factors that preferentially recruit regenerative monocytes that can enhance healing outcomes, tissue regeneration, and biomaterial implant functionality. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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