Bone marrow-derived dendritic cells can process bacteria for MHC-I and MHC-II presentation to T cells
| العنوان: | Bone marrow-derived dendritic cells can process bacteria for MHC-I and MHC-II presentation to T cells |
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| المؤلفون: | Mattias Svensson, Stockinger, B., Wick, Mj |
| المصدر: | Europe PubMed Central Karolinska Institutet |
| مصطلحات موضوعية: | Salmonella typhimurium, Antigens, Bacterial, Cytochalasin D, Immunology, Histocompatibility Antigens Class I, Histocompatibility Antigens Class II, Antigen-Presenting Cells, Bone Marrow Cells, Dendritic Cells, Ammonium Chloride, Mice, Inbred C57BL, Mice, Microscopy, Electron, Escherichia coli, Mice, Inbred CBA, Immunology and Allergy, Animals, Cytoskeleton |
| الوصف: | Dendritic cells can engulf particulate Ags and induce T cell proliferative responses after pulsing with particulate Ag. However, their capacity to process viable Gram-negative bacteria for presentation by MHC-I and MHC-II has not been shown. We therefore characterized the ability of murine bone marrow-derived dendritic cells to process Escherichia coli and Salmonella typhimurium, expressing defined epitopes for presentation by MHC-I and MHC-II molecules. The I-Ak-restricted 46-61 epitope from hen egg white lysozyme (HEL(46-61)) or the Kb-restricted 257-264 epitope from chicken egg OVA (OVA(257-264)) was expressed as fusion proteins in the bacterial cytoplasm as the Crl-HEL and Crl-OVA fusion proteins, respectively. Bacteria expressing Crl-HEL or Crl-OVA, or beads coated with HEL or OVA, were coincubated with murine bone marrow-derived dendritic cells, and Ag processing and presentation were quantitated using T cell hybridomas. The data show that granulocyte-macrophage CSF-stimulated dendritic cells can process live intact Gram-negative bacteria for peptide presentation by MHC-I and MHC-II. Cytochalasin D inhibition studies revealed that processing for both MHC-I and MHC-II presentation required cytoskeletal rearrangements. Processing for MHC-I and MHC-II presentation was inhibited by ammonium chloride, suggesting that acidic compartments were required. Thus, granulocyte-macrophage CSF-stimulated murine bone marrow dendritic cells are capable of processing exogenous particulate Ags, including bacteria with no known mechanism for phagosomal escape, for peptide presentation by both MHC-I and MHC-II. These data suggest that dendritic cells may be important in priming both CD4+ and CD8+ T cells to bacterial Ags. |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b98dcefc3eb4c01bb1642e704e2ba78Test http://europepmc.org/abstract/med/9126984Test |
| رقم الانضمام: | edsair.doi.dedup.....4b98dcefc3eb4c01bb1642e704e2ba78 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |