Production and Characterization of Monoclonal Antibodies against Fumonisin B1
| العنوان: | Production and Characterization of Monoclonal Antibodies against Fumonisin B1 |
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| المؤلفون: | Feng-Yih Yu, Fun S. Chu |
| المصدر: | Food and Agricultural Immunology. 11:297-306 |
| بيانات النشر: | Informa UK Limited, 1999. |
| سنة النشر: | 1999 |
| مصطلحات موضوعية: | Fumonisin B1, biology, medicine.drug_class, Toxin, medicine.medical_treatment, Immunology, Hemocyanin, Monoclonal antibody, medicine.disease_cause, Horseradish peroxidase, Molecular biology, Isotype, Microbiology, chemistry.chemical_compound, chemistry, Cell culture, biology.protein, medicine, Antibody, Agronomy and Crop Science, Food Science |
| الوصف: | Monoclonal antibodies (mAb) against fumonisin B1 (FmB1) were produced from a stable hybridoma cell line, 9C11E6, generated by the fusion of P3/NS-1/1-AG4-1 myeloma cells with spleen cells isolated from a BALB/c mouse immunized with FmB1-keyhole limpet hemocyanin. The 9C11E6 mAb belong to the immunoglobulin G1 (kappa chain) isotype with the highest specificity for FmB3. A direct competitive enzyme-linked immunosorbent assay (dc-ELISA) and an indirect competitive ELISA (idc-ELISA) were established for antibody characterization and toxin analysis. The concentrations causing 50% inhibition (IC 50 ) of binding of FmB1- horseradish peroxidase with the antibodies by FmB1, FmB2, and FmB3 in the dc-ELISA were found to be 29.1, 17.5, and 4.1 ng ml -1 , respectively. The IC 50 values of the binding of 9C11E6 mAb to the solid-phase FmB1-ovalbumin by free FmB1, FmB2, and FmB3 in the idcELISA were found to be 20.1, 13.2, and 16.8 ng ml -1 , respectively. The average recovery of FmB1 (50-2000 ng g -1 ) added to cornmeal... |
| تدمد: | 1465-3443 0954-0105 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::6f813416b0e52aaa111381fa23b3b325Test https://doi.org/10.1080/09540109999681Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........6f813416b0e52aaa111381fa23b3b325 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14653443 09540105 |
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