التفاصيل البيبلوغرافية
| العنوان: |
Development of a Prototype Immunohistochemistry Assay to Measure Programmed Death Ligand-1 Expression in Tumor Tissue. |
| المؤلفون: |
Dolled-Filhart, Marisa1 marisa.dolled-filhart@merck.com, Locke, Darren2,3, Murphy, Tiffany4, Lynch, Frank5, Yearley, Jennifer H.6, Frisman, Dennis7, Pierce, Robert1,8, Weiner, Russell1,9, Wu, Dianna1,10, Emancipator, Kenneth1 |
| المصدر: |
Archives of Pathology & Laboratory Medicine. Nov2016, Vol. 140 Issue 11, p1259-1266. 8p. 4 Color Photographs, 1 Chart, 4 Graphs. |
| مصطلحات موضوعية: |
*THERAPEUTIC use of monoclonal antibodies, *CELL lines, *FLOW cytometry, *IMMUNOHISTOCHEMISTRY, *LUNG cancer, *RESEARCH methodology, *MELANOMA, *PATIENT selection, *INDIVIDUALIZED medicine, *DESCRIPTIVE statistics, *IMMUNOLOGIC receptors |
| مستخلص: |
* Context.--With the abundance of therapeutics targeted against programmed death receptor-1 and its ligand (PD-L1) that are currently approved or in clinical development, there is interest in identifying those patients most likely to respond to these drugs. Expression of PD-L1 may be an indicator of an initial and robust inflammatory response to the presence of tumor cells. Therefore, tumors that express PD-L1 may be the most likely to respond to therapies that interrupt the negative feedback mechanism that leads to PD-L1 upregulation. Objective.--To develop a prototype immunohistochemistry assay using the anti-PD-L1 antibody clone 22C3. Design.--The assay was developed and optimized using commercially available reagents and archival tumor-bank tissue. Results.--The optimized immunohistochemistry method had high precision and reproducibility. Using the proto type assay in 142 non-small cell lung cancer and 79 melanoma archival tumor-bank tissue samples, PD-L1 staining was observed at the plasma membrane of nucleated tumor and nontumor cells and, in some cases, as a distinct lichenoid pattern at the tumor-stroma border. Using a preliminary scoring method, 56% (80 of 142) of non-small cell lung cancer and 53% (42 of 79) of melanoma samples were defined as PD-L1+ based on a modified H-score of 1 or more or the presence of a distinctive staining pattern at the tumor-stroma interface. Conclusions.--The immunohistochemistry assay using the anti-PD-L1 antibody 22C3 merits further investigation in clinical trials and prevalence assessments to further understand the prognostic and predictive value of PD-L1 expression in cancer. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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