التفاصيل البيبلوغرافية
| العنوان: |
A human papillomavirus 8 E7 protein produced in plants is able to trigger the mouse immune system and delay the development of skin lesions. |
| المؤلفون: |
Noris, Emanuela, Poli, Alice, Cojoca, Rodica, Rittà, Massimo, Cavallo, Federica, Vaglio, Stany, Matic, Slavica, Landolfo, Santo |
| المصدر: |
Archives of Virology; Apr2011, Vol. 156 Issue 4, p587-595, 9p |
| مصطلحات موضوعية: |
PAPILLOMAVIRUSES, NICOTIANA benthamiana, TOBACCO mosaic virus, IMMUNE system, SKIN diseases, GENE expression in plants, PLANT extracts, PLANT genetics, LABORATORY mice |
| مستخلص: |
We investigated the potential of Nicotiana benthamiana to express the E7 protein of human papillomavirus 8 (HPV-8), a paradigm genotype among cutaneous HPVs. The protein, modified in its putative pRb-binding domain (E7), was transiently expressed in leaves following infiltration with agrobacteria carrying either a binary vector combined with silencing suppressor constructs or replicating tobacco mosaic virus (TMV)-based vectors with different targeting signals. HPV-8 E7 yields ranged from 250 ng to 4.6 mg per gram of fresh leaf tissue. The highest yields were obtained with TMV-based vectors targeting the antigen to the apoplast. HPV8-CER (H2) mice transformed with the complete early region of HPV-8 showed a delay in the onset of skin papillomatous lesions and produced E7-specific immunoglobulins G when inoculated subcutaneously with leaf extracts expressing E7. Furthermore, we demonstrated that the plant-made HPV-8 E7 induced a specific cytotoxic response in C57BL/6 (H2) mice. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
