المؤلفون: Martorelli, Luisina, Garbaccio, Sergio Gabriel, Vilte, Daniel Alejandro, Albanese, Adriana Andrea, Mejías, María Pilar, Palermo, Marina Sandra, Mercado, Elsa Cristina, Ibarra, Cristina E., Cataldi, Angel Adrian

المصدر: PLoS One 12(1) : 2017

مصطلحات موضوعية: Ternero, Respuesta Inmunológica, Vacunación, Antígenos Bacterianos, Escherichia Coli, Calves, Immune Response, Vaccination, Bacterial Antigens, Toxina Shiga

الوصف: Ruminants are the primary reservoir of Shiga-toxin producing Escherichia coli (STEC) O157:H7 and the main source of infection for humans. The aim of this study was to assess the immunogenic properties of a candidate vaccine consisting on the recombinant proteins of E. coli O157:H7 IntiminC280, the carboxy-terminal fraction of Intimin γ, EspB and the fusion protein between the B subunit of Stx2 and Brucella Lumazine Synthase (BLS)(BLS-Stx2B), in Holstein Fresian calves.To accomplish this goal we vaccinated calves with two doses of different vaccine formulations: 2 antigens (IntiminC280, EspB), 3 antigens (IntiminC280, EspB, BLS-Stx2B), BLS-Stx2B alone and a control non-vaccinated group. All antigens were expressed as recombinant proteins in E. coli. Specific IgG titres increased in vaccinated calves and the inclusion of BLS-Stx2B in the formulation seems to have a stimulatory effect on the humoral response to IntiminC280 and EspB after the booster. The neutralizing activity of antibodies against these two antigens was assessed in Red Blood Cell lysis assays and adherence to Hep-2 cells as a correlate of T3SS activity. Both sera from animals vaccinated with 2 or 3 antigens inhibited both virulence properties. Serological response to Stx2 was observed in animals vaccinated only with BLS-Stx2B and with 3 antigens and neutralization of Stx2 cytotoxicity was also observed in both groups. In conclusion, immunization of calves with BLS-Stx2B, IntiminC280 and EspB elicited a potent humoral response able to neutralize Shiga toxin 2 cytotoxity and the T3SS virulence properties in vitro. These results suggest that this formulation is a good candidate vaccine to reduce STEC shedding in cattle and needs to be further assessed in vivo ; Inst. de Patobiología ; Fil: Martorelli, Luisina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina ; Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina ; Fil: Vilte, Daniel ...

وصف الملف: application/pdf

العلاقة: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0169422Test; http://hdl.handle.net/20.500.12123/491Test; https://doi.org/10.1371/journal.pone.0169422Test

الإتاحة: https://doi.org/20.500.12123/491Test
https://doi.org/10.1371/journal.pone.0169422Test
https://hdl.handle.net/20.500.12123/491Test
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0169422Test

المؤلفون: Blanco, Federico Carlos, Bianco, María Veronica, Meikle, Virginia, Garbaccio, Sergio Gabriel, Vagnoni, Lucas Emilio, Cataldi, Angel Adrian, Bigi, Fabiana

المصدر: RIA 37 (2) : 189-192 (agosto 2011)

مصطلحات موضوعية: Ganado Bovino, Enfermedades de los Animales, Mycobacterium Bovis, Respuesta Inmunológica, Ternero, Infección Experimental, Cattle, Animal Diseases, Immune Response, Calves, Experimental Infection, Mycobacterium Bovis Infections, Infección por Mycobacterium Bovis

الوصف: La tuberculosis bovina es una enfermedad zoonótica producida por Mycobacterium bovis que afecta a animales de producción causando pérdidas económicas y representa, además, un problema para la salud pública. El conocimiento de la respuesta inmune inducida en el ganado bovino (el principal hospedador de M. bovis), durante la tuberculosis bovina es esencial para el desarrollo exitoso de medidas de control de la enfermedad. En este trabajo se determinó el perfil de respuesta celular que se desarrolla en terneros experimentalmente infectados con variadas dosis de M. bovis. Se observó que la respuesta celular CD4 es independiente de la dosis de inóculo, mientras que la respuesta celular CD8 es mínima cuando se infectan los animales a bajas dosis. También se observó que el nivel de producción de interferón gama (IFN-ɣ) por células monocíticas de sangre periférica no se asocia a la dosis de inóculo. ; Mycobacterium bovis, the causative agent of bovine tuberculosis, infects animals of agricultural importance and others mammals, including humans. Therefore, this disease causes significant economical losses and is a public health hazard. To better understand the immune response against M. bovis in cattle, in this study we characterized the immunological profile towards M. bovis antigens in cattle experimentally infected at various doses of a virulent M. bovis strain by measurement of IFN-ɣ production in peripheral blood mononuclear cells and by determining which lymphocyte subsets are involved in recall responses of peripheral blood mononuclear cells from infected cattle to M. bovis antigens. We found that the level of CD4 cell activation is similar among infected group irrespectively of the doses used to infect the animals, while activation of CD8 cells is dose-dependent. We also found that the production of IFN-ɣ in peripheral blood mononuclear cells after specific stimuli is not associated with the bacterial counts in the infectious doses. ; Instituto de Biotecnología ; Fil: Blanco, Federico Carlos. Instituto Nacional ...

وصف الملف: application/pdf

العلاقة: http://ria.inta.gob.ar/sites/default/files/numeros/ria37-2-2011_0.pdfTest; http://hdl.handle.net/20.500.12123/6410Test

الإتاحة: https://doi.org/20.500.12123/6410Test
https://hdl.handle.net/20.500.12123/6410Test
http://ria.inta.gob.ar/sites/default/files/numeros/ria37-2-2011_0.pdfTest