دورية أكاديمية
Immune response in calves vaccinated with type three secretion system antigens and shiga toxin 2B subunit of Escherichia coli O157:H7
| العنوان: | Immune response in calves vaccinated with type three secretion system antigens and shiga toxin 2B subunit of Escherichia coli O157:H7 |
|---|---|
| المؤلفون: | Martorelli, Luisina, Garbaccio, Sergio Gabriel, Vilte, Daniel Alejandro, Albanese, Adriana Andrea, Mejías, María Pilar, Palermo, Marina Sandra, Mercado, Elsa Cristina, Ibarra, Cristina E., Cataldi, Angel Adrian |
| المصدر: | PLoS One 12(1) : 2017 |
| سنة النشر: | 2017 |
| المجموعة: | Inta Digital (ID - Instituto Nacional de Tecnología Agropecuaria) |
| مصطلحات موضوعية: | Ternero, Respuesta Inmunológica, Vacunación, Antígenos Bacterianos, Escherichia Coli, Calves, Immune Response, Vaccination, Bacterial Antigens, Toxina Shiga |
| الوصف: | Ruminants are the primary reservoir of Shiga-toxin producing Escherichia coli (STEC) O157:H7 and the main source of infection for humans. The aim of this study was to assess the immunogenic properties of a candidate vaccine consisting on the recombinant proteins of E. coli O157:H7 IntiminC280, the carboxy-terminal fraction of Intimin γ, EspB and the fusion protein between the B subunit of Stx2 and Brucella Lumazine Synthase (BLS)(BLS-Stx2B), in Holstein Fresian calves.To accomplish this goal we vaccinated calves with two doses of different vaccine formulations: 2 antigens (IntiminC280, EspB), 3 antigens (IntiminC280, EspB, BLS-Stx2B), BLS-Stx2B alone and a control non-vaccinated group. All antigens were expressed as recombinant proteins in E. coli. Specific IgG titres increased in vaccinated calves and the inclusion of BLS-Stx2B in the formulation seems to have a stimulatory effect on the humoral response to IntiminC280 and EspB after the booster. The neutralizing activity of antibodies against these two antigens was assessed in Red Blood Cell lysis assays and adherence to Hep-2 cells as a correlate of T3SS activity. Both sera from animals vaccinated with 2 or 3 antigens inhibited both virulence properties. Serological response to Stx2 was observed in animals vaccinated only with BLS-Stx2B and with 3 antigens and neutralization of Stx2 cytotoxicity was also observed in both groups. In conclusion, immunization of calves with BLS-Stx2B, IntiminC280 and EspB elicited a potent humoral response able to neutralize Shiga toxin 2 cytotoxity and the T3SS virulence properties in vitro. These results suggest that this formulation is a good candidate vaccine to reduce STEC shedding in cattle and needs to be further assessed in vivo ; Inst. de Patobiología ; Fil: Martorelli, Luisina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina ; Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina ; Fil: Vilte, Daniel ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0169422Test; http://hdl.handle.net/20.500.12123/491Test; https://doi.org/10.1371/journal.pone.0169422Test |
| DOI: | 10.1371/journal.pone.0169422 |
| الإتاحة: | https://doi.org/20.500.12123/491Test https://doi.org/10.1371/journal.pone.0169422Test https://hdl.handle.net/20.500.12123/491Test http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0169422Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.93F8E5A9 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pone.0169422 |
|---|